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The map label for this gene is gcvT

Identifier: 126697216

GI number: 126697216

Start: 1604061

End: 1605173

Strand: Direct

Name: gcvT

Synonym: P9301_18781

Alternate gene names: 126697216

Gene position: 1604061-1605173 (Clockwise)

Preceding gene: 126697215

Following gene: 126697217

Centisome position: 97.7

GC content: 31.27

Gene sequence:

>1113_bases
ATGGATTTGCGAAAAAGTCCTCTTTATTCAAAATATATTGAATCCAATGCAAAATTAGTCGATTTTGCAGGTTGGGAAAT
GCCTATATCATTTTCAGGATTAATTAAAGAGCATGAATCAGTTAGATCTTCAGCAGGATTATTTGATATTTCTCACATGG
GTGTGATATCTGTTAAGGGAATCAATCCAAAGGATTATATTCAAAAATTTTTTCCAACTAATTTATACTCCTTTTCTGAA
GGTCAGGGGCTCTATACAGTTATGCTTAATGATAAAGGAGGAATAATAGATGACTTAATAATTTATGACCTTGGTATTCA
AGAAAATGACATAACAGAATTATTGTTAATAGTTAATGCAAGTAGATATGAAGAAGATTTTCAGTGGATAAAAAATAATT
TAAATAAAGATGAAATTTCGATAACAAACTTTAAAAAAGCCAAAGTACTTTTAGCACTACAGGGAAAAAAATCATTCGAT
TTATTTGAAGAATGGATTGATTCTTCGATCTCACATATCCCTAACTTTGGATGCGAATATAAAATTTTTGAACATATTTC
ACCTAAAGAAAAAATTTTCTTTTCAAAGACAGGATATACGGGGGAAAATGGTCTAGAAATACTTTTATCTAAAAAAGCAG
CAATTAATTTATGGGACTTCTCAATTTCCAAAAATGTTGCACCTTGTGGTTTAGGAGCTAGAGATACTCTTAGACTGGAA
GCAGGCATGCATCTTTATGGTCAAGACATAAATGAAGAAACTTCTCCATATGAAGCAGGGTTAGGCTGGCTAGTACATCT
AGAAAATAATCACGAATTCTTTGGAAGAAGATTTCTTGAAGAACAGTCAAGATTAGGTATTCAAAAAAAGTTAGTTGGTC
TTTTTATAGAAGGTAAAGCAATAGGAAGAAAGGGTTGCACAGTTCTTAAAGGCGAAGAAAATATTGGGACTATCACAAGC
GGAAGTTGGTCGCCAACTAAACAACAAGCTATAGCTTTTGCATACATCAACACTTCGCATGCACTAATAAATAATGAAGT
TCAAGTATTAATTAGAGGCAAAAAATTCAAAGGGGTTATAACAAAAAGAGCGTTTTATAAAAAAAATTATTAA

Upstream 100 bases:

>100_bases
CAGCCAAAGTACTTAGAAGCTTAATTTTGTCATTAGAAAATATGCAATAAAAAATTTCTAAACTACAGTGGAAAAATACT
AAATACAAACTAAATATTTC

Downstream 100 bases:

>100_bases
CTAAATTTACCCTTGAAGAATTATTATAAGTTATTGATAAATAAATCATACTTAGATGAGAAACAAAATTTGCAAAGAAC
TCAATAATACAGATATTGGT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MDLRKSPLYSKYIESNAKLVDFAGWEMPISFSGLIKEHESVRSSAGLFDISHMGVISVKGINPKDYIQKFFPTNLYSFSE
GQGLYTVMLNDKGGIIDDLIIYDLGIQENDITELLLIVNASRYEEDFQWIKNNLNKDEISITNFKKAKVLLALQGKKSFD
LFEEWIDSSISHIPNFGCEYKIFEHISPKEKIFFSKTGYTGENGLEILLSKKAAINLWDFSISKNVAPCGLGARDTLRLE
AGMHLYGQDINEETSPYEAGLGWLVHLENNHEFFGRRFLEEQSRLGIQKKLVGLFIEGKAIGRKGCTVLKGEENIGTITS
GSWSPTKQQAIAFAYINTSHALINNEVQVLIRGKKFKGVITKRAFYKKNY

Sequences:

>Translated_370_residues
MDLRKSPLYSKYIESNAKLVDFAGWEMPISFSGLIKEHESVRSSAGLFDISHMGVISVKGINPKDYIQKFFPTNLYSFSE
GQGLYTVMLNDKGGIIDDLIIYDLGIQENDITELLLIVNASRYEEDFQWIKNNLNKDEISITNFKKAKVLLALQGKKSFD
LFEEWIDSSISHIPNFGCEYKIFEHISPKEKIFFSKTGYTGENGLEILLSKKAAINLWDFSISKNVAPCGLGARDTLRLE
AGMHLYGQDINEETSPYEAGLGWLVHLENNHEFFGRRFLEEQSRLGIQKKLVGLFIEGKAIGRKGCTVLKGEENIGTITS
GSWSPTKQQAIAFAYINTSHALINNEVQVLIRGKKFKGVITKRAFYKKNY
>Mature_370_residues
MDLRKSPLYSKYIESNAKLVDFAGWEMPISFSGLIKEHESVRSSAGLFDISHMGVISVKGINPKDYIQKFFPTNLYSFSE
GQGLYTVMLNDKGGIIDDLIIYDLGIQENDITELLLIVNASRYEEDFQWIKNNLNKDEISITNFKKAKVLLALQGKKSFD
LFEEWIDSSISHIPNFGCEYKIFEHISPKEKIFFSKTGYTGENGLEILLSKKAAINLWDFSISKNVAPCGLGARDTLRLE
AGMHLYGQDINEETSPYEAGLGWLVHLENNHEFFGRRFLEEQSRLGIQKKLVGLFIEGKAIGRKGCTVLKGEENIGTITS
GSWSPTKQQAIAFAYINTSHALINNEVQVLIRGKKFKGVITKRAFYKKNY

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=379, Percent_Identity=29.287598944591, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI257796258, Length=350, Percent_Identity=29.4285714285714, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI257796254, Length=374, Percent_Identity=28.3422459893048, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI257796256, Length=307, Percent_Identity=29.3159609120521, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI24797151, Length=394, Percent_Identity=22.8426395939086, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI197927446, Length=333, Percent_Identity=21.6216216216216, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI21361378, Length=333, Percent_Identity=21.6216216216216, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI194306651, Length=372, Percent_Identity=22.5806451612903, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789272, Length=377, Percent_Identity=35.5437665782493, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17560118, Length=383, Percent_Identity=30.8093994778068, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71994045, Length=378, Percent_Identity=24.0740740740741, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71994052, Length=378, Percent_Identity=24.0740740740741, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32563613, Length=399, Percent_Identity=23.3082706766917, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320222, Length=391, Percent_Identity=28.9002557544757, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI20129441, Length=397, Percent_Identity=29.2191435768262, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_PROM0 (A3PFH6)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092102.1
- ProteinModelPortal:   A3PFH6
- SMR:   A3PFH6
- STRING:   A3PFH6
- GeneID:   4912738
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18781
- eggNOG:   COG0404
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- BioCyc:   PMAR167546:P9301ORF_1920-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 41870; Mature: 41870

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRKSPLYSKYIESNAKLVDFAGWEMPISFSGLIKEHESVRSSAGLFDISHMGVISVKG
CCCCCCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEEEC
INPKDYIQKFFPTNLYSFSEGQGLYTVMLNDKGGIIDDLIIYDLGIQENDITELLLIVNA
CCHHHHHHHHCCCCCEECCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCEEEEEEEEC
SRYEEDFQWIKNNLNKDEISITNFKKAKVLLALQGKKSFDLFEEWIDSSISHIPNFGCEY
HHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
KIFEHISPKEKIFFSKTGYTGENGLEILLSKKAAINLWDFSISKNVAPCGLGARDTLRLE
EHHHCCCCCHHEEEEECCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEE
AGMHLYGQDINEETSPYEAGLGWLVHLENNHEFFGRRFLEEQSRLGIQKKLVGLFIEGKA
CCCEEECCCCCCCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHEEEEEEECCC
IGRKGCTVLKGEENIGTITSGSWSPTKQQAIAFAYINTSHALINNEVQVLIRGKKFKGVI
CCCCCCEEEECCCCCCEEECCCCCCCHHHEEEEEEEECCCEEECCCEEEEEECCCCCCHH
TKRAFYKKNY
HHHHHHCCCC
>Mature Secondary Structure
MDLRKSPLYSKYIESNAKLVDFAGWEMPISFSGLIKEHESVRSSAGLFDISHMGVISVKG
CCCCCCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEEEC
INPKDYIQKFFPTNLYSFSEGQGLYTVMLNDKGGIIDDLIIYDLGIQENDITELLLIVNA
CCHHHHHHHHCCCCCEECCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCEEEEEEEEC
SRYEEDFQWIKNNLNKDEISITNFKKAKVLLALQGKKSFDLFEEWIDSSISHIPNFGCEY
HHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
KIFEHISPKEKIFFSKTGYTGENGLEILLSKKAAINLWDFSISKNVAPCGLGARDTLRLE
EHHHCCCCCHHEEEEECCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEE
AGMHLYGQDINEETSPYEAGLGWLVHLENNHEFFGRRFLEEQSRLGIQKKLVGLFIEGKA
CCCEEECCCCCCCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHEEEEEEECCC
IGRKGCTVLKGEENIGTITSGSWSPTKQQAIAFAYINTSHALINNEVQVLIRGKKFKGVI
CCCCCCEEEECCCCCCEEECCCCCCCHHHEEEEEEEECCCEEECCCEEEEEECCCCCCHH
TKRAFYKKNY
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA