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The map label for this gene is mesJ [H]

Identifier: 126697179

GI number: 126697179

Start: 1564872

End: 1565882

Strand: Direct

Name: mesJ [H]

Synonym: P9301_18411

Alternate gene names: 126697179

Gene position: 1564872-1565882 (Clockwise)

Preceding gene: 126697176

Following gene: 126697183

Centisome position: 95.31

GC content: 28.68

Gene sequence:

>1011_bases
ATGACTGATAAAAAATTAAGTCAGAAAAATTGGTCACCATGGCATCATCAGCTTCATAAGGAGATTCTTACCAAAAAAAT
ATTAATTCCCAAAGGATCCAATATTTTAATAAGTGTTTCCGGGGGTCAAGACTCAATGACCTTATTAACCTTAATTAATG
ACCTAAAAAAACTACATAACTGGTCTATTAGTGTTTGGCATGGTGATCATCAGTGGCACGAAAAATCATCACTATATGCT
CTTGAATTAAAAGATTATTGCGAAGATAAAAATATTTCATTCTCTTATGATCAAGCAAATAAAGAAAGTATTTCTTCAGA
AGAAAAAGCACGAGAATGGAGATATAAAAAATTATGTGAAAGAGCCAAAACTTTATTAAATACAAACCAGCAAAAACATA
ATATTTATTTGTTAACTGGTCACACGAGTAGTGATAATGCAGAAACATTTATCCTAAATTTATCTAGAGGAAGCAATTTT
GCTGGCCTGAGTAATATTGAAAGTAAAAGATTAATTGAAAATCAAATTTTTTTAATAAGACCAATATTAATTTTCAGTCG
GGAAGATACAAAACAATTTTGCAATGATATGAAGATCCCAGTCTGGGAAGATCCTACAAATTCAGATCTTAAATTAAAAA
GAAATTTAGTAAGAAAAAAAATTATTCCTACCTTAGAAGTCATCTATCCTGGTTGTTCTGAAAGGATAAATAATTTTTCC
CAAAAAATGAGCAATTACAATAATGAACGTAATGATCTTAGTGAACTAGCATACCTGTATTGTAGAGATGTAAAAGGTAT
CGATAGGAATCTCCTAAATGGTATGTGTATTCAAGCGAGGTGCACAATCTTAAATAGATTTTTAAAAGAAATATCTGCAA
AGCAGTTTAGTTCTAAAAATCTCACAAAATTGGCAACTTCAATTTATGAAAAAAATAAAGGGCAAATTAATCTTCAAGAG
TTTTTAAAAATTGTTTGGGATAAAAACTATATAAATTTTGAAAAAAGTTAA

Upstream 100 bases:

>100_bases
ATTAATCAGACTCATTTACTTAATGAATTTATTACCCGATTGTGACATTGTTTTAGTAAAACAGTGCATTTTTTAAAAAA
TATTATCTGAGCAACACAAA

Downstream 100 bases:

>100_bases
GATGTTACAGCAGATCTTCTTCTTCTAGTTCTACCCTCAAATGAATCTTCTGTAACAGTCTCAGCTGATGGATTCTGTGA
ACCTTTTGGACTTTTTTGGG

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTDKKLSQKNWSPWHHQLHKEILTKKILIPKGSNILISVSGGQDSMTLLTLINDLKKLHNWSISVWHGDHQWHEKSSLYA
LELKDYCEDKNISFSYDQANKESISSEEKAREWRYKKLCERAKTLLNTNQQKHNIYLLTGHTSSDNAETFILNLSRGSNF
AGLSNIESKRLIENQIFLIRPILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
QKMSNYNNERNDLSELAYLYCRDVKGIDRNLLNGMCIQARCTILNRFLKEISAKQFSSKNLTKLATSIYEKNKGQINLQE
FLKIVWDKNYINFEKS

Sequences:

>Translated_336_residues
MTDKKLSQKNWSPWHHQLHKEILTKKILIPKGSNILISVSGGQDSMTLLTLINDLKKLHNWSISVWHGDHQWHEKSSLYA
LELKDYCEDKNISFSYDQANKESISSEEKAREWRYKKLCERAKTLLNTNQQKHNIYLLTGHTSSDNAETFILNLSRGSNF
AGLSNIESKRLIENQIFLIRPILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
QKMSNYNNERNDLSELAYLYCRDVKGIDRNLLNGMCIQARCTILNRFLKEISAKQFSSKNLTKLATSIYEKNKGQINLQE
FLKIVWDKNYINFEKS
>Mature_335_residues
TDKKLSQKNWSPWHHQLHKEILTKKILIPKGSNILISVSGGQDSMTLLTLINDLKKLHNWSISVWHGDHQWHEKSSLYAL
ELKDYCEDKNISFSYDQANKESISSEEKAREWRYKKLCERAKTLLNTNQQKHNIYLLTGHTSSDNAETFILNLSRGSNFA
GLSNIESKRLIENQIFLIRPILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFSQ
KMSNYNNERNDLSELAYLYCRDVKGIDRNLLNGMCIQARCTILNRFLKEISAKQFSSKNLTKLATSIYEKNKGQINLQEF
LKIVWDKNYINFEKS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=291, Percent_Identity=24.3986254295533, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 39328; Mature: 39196

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKLSQKNWSPWHHQLHKEILTKKILIPKGSNILISVSGGQDSMTLLTLINDLKKLHN
CCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
WSISVWHGDHQWHEKSSLYALELKDYCEDKNISFSYDQANKESISSEEKAREWRYKKLCE
CEEEEEECCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHH
RAKTLLNTNQQKHNIYLLTGHTSSDNAETFILNLSRGSNFAGLSNIESKRLIENQIFLIR
HHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCHHEEE
PILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
HHHEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
QKMSNYNNERNDLSELAYLYCRDVKGIDRNLLNGMCIQARCTILNRFLKEISAKQFSSKN
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
LTKLATSIYEKNKGQINLQEFLKIVWDKNYINFEKS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
TDKKLSQKNWSPWHHQLHKEILTKKILIPKGSNILISVSGGQDSMTLLTLINDLKKLHN
CCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
WSISVWHGDHQWHEKSSLYALELKDYCEDKNISFSYDQANKESISSEEKAREWRYKKLCE
CEEEEEECCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHH
RAKTLLNTNQQKHNIYLLTGHTSSDNAETFILNLSRGSNFAGLSNIESKRLIENQIFLIR
HHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCHHEEE
PILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
HHHEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
QKMSNYNNERNDLSELAYLYCRDVKGIDRNLLNGMCIQARCTILNRFLKEISAKQFSSKN
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
LTKLATSIYEKNKGQINLQEFLKIVWDKNYINFEKS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]