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The map label for this gene is smpB

Identifier: 126697146

GI number: 126697146

Start: 1531026

End: 1531520

Strand: Direct

Name: smpB

Synonym: P9301_18081

Alternate gene names: 126697146

Gene position: 1531026-1531520 (Clockwise)

Preceding gene: 126697141

Following gene: 126697153

Centisome position: 93.25

GC content: 28.08

Gene sequence:

>495_bases
ATGGCAAAAAATTCAAAAAAAGTTAAAAGCAATACTAATAAAGCAAATAATTTTAAACTTCTAGCTGAGAATAGATATGC
AAAATTTCAATATGCAATATCTGAGACAATCGAAGCTGGAATTGAGCTTTTAGGGACTGAAGTAAAGTCCATTAGAAACG
GAAAAGCAAATTTAAGAGACGGATACTGTTCATTCAGGGATGGTGAGATCTTATTATTAAATGTTCACATTTCACCACAT
AAAAATGTGGGATCTTTCTTTAATCATGATCCATTAAGAAATAGAAAGTTGCTATTACATAAAAAAGAAATAATAAAAAT
GAAATCTAATACTGAAAAAAAAGGAATGACTATTGTTCCATTATCTCTTTATTTAAAAGGTTCATGGATAAAAATAACTT
TAGGAGTCGGAAAAGGGAAAAAATTGCATGATAAACGACAAGACGAGAAACAAAAAAGTATTAAAAAAGAAATCAACTCT
GCACTAAAAAGATAA

Upstream 100 bases:

>100_bases
CCGAAGAGGTAAGTCATTATCGCCTATATTAGAGGATATTATTGCCATAATGAAAGGTTAATTGCAATTGTTCTGAGTAG
AAAGACTTTTTACAACTAAA

Downstream 100 bases:

>100_bases
AAATATTATGCACAATTATTGAGACTATCAATCTAAAGTGACTGAATTTGGAGGTGGGGAAGGATCTGGATCTGCAATTA
ACATATGCTGTAATACAGTT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAKNSKKVKSNTNKANNFKLLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRDGYCSFRDGEILLLNVHISPH
KNVGSFFNHDPLRNRKLLLHKKEIIKMKSNTEKKGMTIVPLSLYLKGSWIKITLGVGKGKKLHDKRQDEKQKSIKKEINS
ALKR

Sequences:

>Translated_164_residues
MAKNSKKVKSNTNKANNFKLLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRDGYCSFRDGEILLLNVHISPH
KNVGSFFNHDPLRNRKLLLHKKEIIKMKSNTEKKGMTIVPLSLYLKGSWIKITLGVGKGKKLHDKRQDEKQKSIKKEINS
ALKR
>Mature_163_residues
AKNSKKVKSNTNKANNFKLLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRDGYCSFRDGEILLLNVHISPHK
NVGSFFNHDPLRNRKLLLHKKEIIKMKSNTEKKGMTIVPLSLYLKGSWIKITLGVGKGKKLHDKRQDEKQKSIKKEINSA
LKR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=132, Percent_Identity=45.4545454545455, Blast_Score=124, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_PROM0 (A3PFA6)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092032.1
- ProteinModelPortal:   A3PFA6
- SMR:   A3PFA6
- STRING:   A3PFA6
- GeneID:   4911512
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18081
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   PMAR167546:P9301ORF_1847-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18704; Mature: 18573

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNSKKVKSNTNKANNFKLLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRD
CCCCCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYCSFRDGEILLLNVHISPHKNVGSFFNHDPLRNRKLLLHKKEIIKMKSNTEKKGMTIVP
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCEEEEE
LSLYLKGSWIKITLGVGKGKKLHDKRQDEKQKSIKKEINSALKR
EEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKNSKKVKSNTNKANNFKLLAENRYAKFQYAISETIEAGIELLGTEVKSIRNGKANLRD
CCCCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYCSFRDGEILLLNVHISPHKNVGSFFNHDPLRNRKLLLHKKEIIKMKSNTEKKGMTIVP
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCEEEEE
LSLYLKGSWIKITLGVGKGKKLHDKRQDEKQKSIKKEINSALKR
EEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA