Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 126697145

GI number: 126697145

Start: 1529915

End: 1530973

Strand: Reverse

Name: ruvB

Synonym: P9301_18071

Alternate gene names: 126697145

Gene position: 1530973-1529915 (Counterclockwise)

Preceding gene: 126697147

Following gene: 126697144

Centisome position: 93.25

GC content: 32.67

Gene sequence:

>1059_bases
ATGGCAATAATATCCTCTAATATAGGCGATAATGACTTACCTCTTCGGAAAAAAGAGCTGAGGTTAGTTGATTCAAAAAT
CATTCCAGAAGAAAAGAGAAATAATAATCTTAACTTGGCCCGGCCTCTTACTTTAAAAGAATTTATTGGCCAAGAACAAC
TTAAATCTTCTTTAAGAATAGCTATAGATGCATCAATTTTTAGAAAAGAACCCTTGGAACATATTCTTTTATATGGACAG
CCTGGTCTAGGTAAGACGACTCTTGCTTTTTTGATAGCCCATGAATTGAATACAAAATGTAAGATAGCTACTGCACCAGC
AATTGAAAGACCAAGAGATATTGTAGGTTTACTTCTTGGATTAAAAGAAGGTGAAGTTTTATTTATCGATGAGATACATC
GCTTAAATAGGTTAACTGAAGAGTTGTTGTATTCTGCAATGGAAGATTTTAGACTCGATTTAACTATGGGAGCTAACAAA
GGAACCCGTTGCAGAACAATTAATCTTCCTAGGTTTACTCTGATTGGCGCGACAACTAAATTAGCCTCAATAAGTGCACC
TCTAAGAGATAGATTTGGTATATCTCAGAAAATTGAATTTTATAATTATGATGAATTAAAACAAATTCTTCTTAATTTCT
CCCGATTAATAAATCTCAATTTAGATGATGAAGCATCCTATGATTTAGCAAAGATATCTCGAGGGACTCCAAGAATTGCT
TTAAGGTTATTAAGACGAGTGAGAGATTATGCTCAAGTTGTTATGAAAACCAATACTATCTCCGTGAATTTAATAAAAAA
AGCCTTAAATTCTTACCAAATAGATGATAAAGGATTGGATTCTTTAGATAGACACTATTTATCTTTTATTAACCAAAACA
ATAATATTCCAATTGGCCTTGATTCAATTGCAGCAGGCTTGGGTGATGATTCTTCAATGTTAGAATTTGTAGTTGAGCCA
TATCTTATAAAAATTGGTTTCCTCACTAGAACTCCTCGAGGAAGATTGCTTACTGCTTTAGGAAAAAAGTACATTGATTC
AAAAAATGATGACTTTTAG

Upstream 100 bases:

>100_bases
ATTTGCTTTATTAGTATTGCTTTTAACTTTTTTTGAATTTTTTGCCATTTTAGTTGTAAAAAGTCTTTCTACTCAGAACA
ATTGCAATTAACCTTTCATT

Downstream 100 bases:

>100_bases
AAAAGTAAAGGTTTGTTTTTTATTGATTTTTATTTTTTTGAATATTTTTTATATTGCACCATGCTATTCATTATCTTTGA
GAGGGGATTTGTTTAAAGAT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MAIISSNIGDNDLPLRKKELRLVDSKIIPEEKRNNNLNLARPLTLKEFIGQEQLKSSLRIAIDASIFRKEPLEHILLYGQ
PGLGKTTLAFLIAHELNTKCKIATAPAIERPRDIVGLLLGLKEGEVLFIDEIHRLNRLTEELLYSAMEDFRLDLTMGANK
GTRCRTINLPRFTLIGATTKLASISAPLRDRFGISQKIEFYNYDELKQILLNFSRLINLNLDDEASYDLAKISRGTPRIA
LRLLRRVRDYAQVVMKTNTISVNLIKKALNSYQIDDKGLDSLDRHYLSFINQNNNIPIGLDSIAAGLGDDSSMLEFVVEP
YLIKIGFLTRTPRGRLLTALGKKYIDSKNDDF

Sequences:

>Translated_352_residues
MAIISSNIGDNDLPLRKKELRLVDSKIIPEEKRNNNLNLARPLTLKEFIGQEQLKSSLRIAIDASIFRKEPLEHILLYGQ
PGLGKTTLAFLIAHELNTKCKIATAPAIERPRDIVGLLLGLKEGEVLFIDEIHRLNRLTEELLYSAMEDFRLDLTMGANK
GTRCRTINLPRFTLIGATTKLASISAPLRDRFGISQKIEFYNYDELKQILLNFSRLINLNLDDEASYDLAKISRGTPRIA
LRLLRRVRDYAQVVMKTNTISVNLIKKALNSYQIDDKGLDSLDRHYLSFINQNNNIPIGLDSIAAGLGDDSSMLEFVVEP
YLIKIGFLTRTPRGRLLTALGKKYIDSKNDDF
>Mature_351_residues
AIISSNIGDNDLPLRKKELRLVDSKIIPEEKRNNNLNLARPLTLKEFIGQEQLKSSLRIAIDASIFRKEPLEHILLYGQP
GLGKTTLAFLIAHELNTKCKIATAPAIERPRDIVGLLLGLKEGEVLFIDEIHRLNRLTEELLYSAMEDFRLDLTMGANKG
TRCRTINLPRFTLIGATTKLASISAPLRDRFGISQKIEFYNYDELKQILLNFSRLINLNLDDEASYDLAKISRGTPRIAL
RLLRRVRDYAQVVMKTNTISVNLIKKALNSYQIDDKGLDSLDRHYLSFINQNNNIPIGLDSIAAGLGDDSSMLEFVVEPY
LIKIGFLTRTPRGRLLTALGKKYIDSKNDDF

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=299, Percent_Identity=45.819397993311, Blast_Score=283, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_PROM0 (A3PFA5)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092031.1
- ProteinModelPortal:   A3PFA5
- SMR:   A3PFA5
- STRING:   A3PFA5
- GeneID:   4911511
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18071
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   PMAR167546:P9301ORF_1846-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 39840; Mature: 39709

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIISSNIGDNDLPLRKKELRLVDSKIIPEEKRNNNLNLARPLTLKEFIGQEQLKSSLRI
CCEEECCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCEECCCHHHHHCCHHHHHHHHEE
AIDASIFRKEPLEHILLYGQPGLGKTTLAFLIAHELNTKCKIATAPAIERPRDIVGLLLG
EEEHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHC
LKEGEVLFIDEIHRLNRLTEELLYSAMEDFRLDLTMGANKGTRCRTINLPRFTLIGATTK
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCCEEEEECHHH
LASISAPLRDRFGISQKIEFYNYDELKQILLNFSRLINLNLDDEASYDLAKISRGTPRIA
HHHCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHHHEECCCCCCCCCCHHHHCCCCHHHH
LRLLRRVRDYAQVVMKTNTISVNLIKKALNSYQIDDKGLDSLDRHYLSFINQNNNIPIGL
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEH
DSIAAGLGDDSSMLEFVVEPYLIKIGFLTRTPRGRLLTALGKKYIDSKNDDF
HHHHHCCCCCHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AIISSNIGDNDLPLRKKELRLVDSKIIPEEKRNNNLNLARPLTLKEFIGQEQLKSSLRI
CEEECCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCEECCCHHHHHCCHHHHHHHHEE
AIDASIFRKEPLEHILLYGQPGLGKTTLAFLIAHELNTKCKIATAPAIERPRDIVGLLLG
EEEHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHC
LKEGEVLFIDEIHRLNRLTEELLYSAMEDFRLDLTMGANKGTRCRTINLPRFTLIGATTK
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCCEEEEECHHH
LASISAPLRDRFGISQKIEFYNYDELKQILLNFSRLINLNLDDEASYDLAKISRGTPRIA
HHHCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHHHEECCCCCCCCCCHHHHCCCCHHHH
LRLLRRVRDYAQVVMKTNTISVNLIKKALNSYQIDDKGLDSLDRHYLSFINQNNNIPIGL
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEH
DSIAAGLGDDSSMLEFVVEPYLIKIGFLTRTPRGRLLTALGKKYIDSKNDDF
HHHHHCCCCCHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA