Click here to switch to the map view.

The map label for this gene is hemF

Identifier: 126697111

GI number: 126697111

Start: 1497382

End: 1498410

Strand: Direct

Name: hemF

Synonym: P9301_17731

Alternate gene names: 126697111

Gene position: 1497382-1498410 (Clockwise)

Preceding gene: 126697100

Following gene: 126697113

Centisome position: 91.2

GC content: 34.99

Gene sequence:

>1029_bases
ATGTTGAAAGAACCTCCTAAAAACTCGAGAGAAAAAACTAAAAATCTCTTATTAACTCTACAAGACAAAATTTGTTCAGG
CCTTGAAAATGTAGATGGCAAAGGGAAATTTACAGAGGAATCCTGGCTAAGAGACGAAGGTGGCGGTGGAAGATCAAGAG
TATTGAAAAATGGTTCTATTTTTGAGCAAGCAGGTGTTAACTTCTCGGAAGTACAGGGAAAAGAATTACCTCAATCTATA
ATCTCTCAAAGACCCGAAGCAAAAGGTCATGAATGGTTTGCCACAGGAACTTCTATGGTTTTGCATCCTAAGAATCCCTA
CATTCCTACAGTGCATCTGAATTATCGATATTTCGAAGCTGGTCCTGTTTGGTGGTTTGGGGGAGGTGCAGATTTAACCC
CTTTTTATCCTTATCTTTCTGATGTAAGGAATTTTCATAATGAACATAAAAAAGCTTGTGAGAAAGTTGATCAAGATTTG
CATAAAGTTTTCAAACCATGGTGTGATGAATATTTCTTCTTGAAGCATAGAAATGAATCTAGAGGCATAGGAGGTATTTT
TTATGATTATCAAGATGGTTCAGGCAATATTTATAGAGGAAATAATCAAAAAGGAGAAGCATCAAAAGCTTCACAAAATA
TTGGCAGATCTAATTTAAATTGGGATGATTTATTTTCATTAGCGGAAAACTGTGGGCAGGCATTCCTCCCTTCATATTTG
CCTATTATTGAAAAAAGAGCTTCTCAAGCATATTCATCGAAGGAAAGAGAATTCCAGCTATATCGAAGAGGTAGATATGT
TGAATTCAATTTAGTTTGGGATAGAGGGACAATTTTTGGACTGCAAACAAATGGCAGAACTGAATCTATATTAATGTCCT
TACCGCCTTTAGCTAGATGGGAATATGGATATAAAGCTAGAAAGAATTCTCGAGAGGAATTTCTTACATCAATATTTACA
AAACCCCAAGATTGGTTAAACGATAAAGAGTTAGAGAAATTCTGTATAGAGAATAATATTTTTGATTAA

Upstream 100 bases:

>100_bases
AACTATCAAATAATAGGCAATAAGGACCCCCTAAGAGAAATTATAAAGAGAAACTTATAATTAACCAAAAAAAATTTTTT
CTTCAAGATTTACTAAATAC

Downstream 100 bases:

>100_bases
AAATTATCGTATAAACTTTTAAAGTATCTAAATAGGTGTTTTAAATAAAGAGCCGTCACTTTTCAATTGAAGTTTTTCTC
CGAGTTCATTTTCTAAAGAG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLKEPPKNSREKTKNLLLTLQDKICSGLENVDGKGKFTEESWLRDEGGGGRSRVLKNGSIFEQAGVNFSEVQGKELPQSI
ISQRPEAKGHEWFATGTSMVLHPKNPYIPTVHLNYRYFEAGPVWWFGGGADLTPFYPYLSDVRNFHNEHKKACEKVDQDL
HKVFKPWCDEYFFLKHRNESRGIGGIFYDYQDGSGNIYRGNNQKGEASKASQNIGRSNLNWDDLFSLAENCGQAFLPSYL
PIIEKRASQAYSSKEREFQLYRRGRYVEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYKARKNSREEFLTSIFT
KPQDWLNDKELEKFCIENNIFD

Sequences:

>Translated_342_residues
MLKEPPKNSREKTKNLLLTLQDKICSGLENVDGKGKFTEESWLRDEGGGGRSRVLKNGSIFEQAGVNFSEVQGKELPQSI
ISQRPEAKGHEWFATGTSMVLHPKNPYIPTVHLNYRYFEAGPVWWFGGGADLTPFYPYLSDVRNFHNEHKKACEKVDQDL
HKVFKPWCDEYFFLKHRNESRGIGGIFYDYQDGSGNIYRGNNQKGEASKASQNIGRSNLNWDDLFSLAENCGQAFLPSYL
PIIEKRASQAYSSKEREFQLYRRGRYVEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYKARKNSREEFLTSIFT
KPQDWLNDKELEKFCIENNIFD
>Mature_342_residues
MLKEPPKNSREKTKNLLLTLQDKICSGLENVDGKGKFTEESWLRDEGGGGRSRVLKNGSIFEQAGVNFSEVQGKELPQSI
ISQRPEAKGHEWFATGTSMVLHPKNPYIPTVHLNYRYFEAGPVWWFGGGADLTPFYPYLSDVRNFHNEHKKACEKVDQDL
HKVFKPWCDEYFFLKHRNESRGIGGIFYDYQDGSGNIYRGNNQKGEASKASQNIGRSNLNWDDLFSLAENCGQAFLPSYL
PIIEKRASQAYSSKEREFQLYRRGRYVEFNLVWDRGTIFGLQTNGRTESILMSLPPLARWEYGYKARKNSREEFLTSIFT
KPQDWLNDKELEKFCIENNIFD

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=338, Percent_Identity=41.1242603550296, Blast_Score=268, Evalue=5e-72,
Organism=Escherichia coli, GI1788777, Length=325, Percent_Identity=49.8461538461538, Blast_Score=328, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6320249, Length=341, Percent_Identity=43.108504398827, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI17864388, Length=333, Percent_Identity=42.9429429429429, Blast_Score=276, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_PROM0 (A3PF71)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091997.1
- ProteinModelPortal:   A3PF71
- SMR:   A3PF71
- STRING:   A3PF71
- GeneID:   4911897
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_17731
- eggNOG:   COG0408
- HOGENOM:   HBG631180
- OMA:   VFKPWCD
- ProtClustDB:   PRK05330
- BioCyc:   PMAR167546:P9301ORF_1809-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 39564; Mature: 39564

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 112-112 BINDING 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEPPKNSREKTKNLLLTLQDKICSGLENVDGKGKFTEESWLRDEGGGGRSRVLKNGSI
CCCCCCCCHHHHHHHHHEEEHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHEECCCCC
FEQAGVNFSEVQGKELPQSIISQRPEAKGHEWFATGTSMVLHPKNPYIPTVHLNYRYFEA
HHHHCCCHHHHCCHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCEEEEEEEEEEC
GPVWWFGGGADLTPFYPYLSDVRNFHNEHKKACEKVDQDLHKVFKPWCDEYFFLKHRNES
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
RGIGGIFYDYQDGSGNIYRGNNQKGEASKASQNIGRSNLNWDDLFSLAENCGQAFLPSYL
CCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHH
PIIEKRASQAYSSKEREFQLYRRGRYVEFNLVWDRGTIFGLQTNGRTESILMSLPPLARW
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCHHHEECCCCHHHH
EYGYKARKNSREEFLTSIFTKPQDWLNDKELEKFCIENNIFD
CCCHHHHCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKEPPKNSREKTKNLLLTLQDKICSGLENVDGKGKFTEESWLRDEGGGGRSRVLKNGSI
CCCCCCCCHHHHHHHHHEEEHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHEECCCCC
FEQAGVNFSEVQGKELPQSIISQRPEAKGHEWFATGTSMVLHPKNPYIPTVHLNYRYFEA
HHHHCCCHHHHCCHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCEEEEEEEEEEC
GPVWWFGGGADLTPFYPYLSDVRNFHNEHKKACEKVDQDLHKVFKPWCDEYFFLKHRNES
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
RGIGGIFYDYQDGSGNIYRGNNQKGEASKASQNIGRSNLNWDDLFSLAENCGQAFLPSYL
CCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHH
PIIEKRASQAYSSKEREFQLYRRGRYVEFNLVWDRGTIFGLQTNGRTESILMSLPPLARW
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCHHHEECCCCHHHH
EYGYKARKNSREEFLTSIFTKPQDWLNDKELEKFCIENNIFD
CCCHHHHCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA