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The map label for this gene is thiF [H]

Identifier: 126697098

GI number: 126697098

Start: 1481810

End: 1482955

Strand: Direct

Name: thiF [H]

Synonym: P9301_17601

Alternate gene names: 126697098

Gene position: 1481810-1482955 (Clockwise)

Preceding gene: 126697096

Following gene: 126697100

Centisome position: 90.25

GC content: 30.37

Gene sequence:

>1146_bases
ATGTCCAACGATATCAAATTTAACTTCTTAAGCTCTGATGAAGAAGAAAGATATCAAAAACATTTAACCCTTAAAGAAAT
AGGTTATGAGGGTCAACTAAATCTTAAAAACAGCTCAGTATTATGCATTGGAGCAGGTGGACTTGGGTCTTCGGTTTTGC
TTTATCTTGCCGCAACAGGAATTGGGAGGATTGGAATCGTAGATAACGATCAAGTCGAAAAGTCTAATCTCCAGAGACAG
ATAATTCATGAAACAAAAACTATTGGCAATCTGAAAATTGATTCTGCCAAGGAAAGAATTAAAAAATTGAATCCTAATTG
TGAAATATTAACCTTTCCAGAAAGAATTAATCCTAAAAATGCCCTTGAATTAATAAATAAGTTTGATGTTATTTGTGATT
GCTCGGATAACTTTAGTACAAGATATTTGATAAATGATTCATGTCTGATATTAAATAAACCCCTTGTATTTGGAAGTGTA
CAAGGCTTCGAAGGACAAGTAAGTGTTTTCAATTTATACAAAAATAGTCCTAATTTAAGAGACTTACTTCCAGAATCACC
TTCAAAAAATGCTGCCCCTAGTTGTGCAGAATACGGCGTTGTAGGCGTTTCAACAGGTTTAATAGGAATTCTTCAGGTTA
ATGAAATAATCAAAATCATTTTGAAAAAAGGTGAAACTTTGGATGGGAAAATTTTAATTTTTGATCTATTAAAGATGAAT
ATAAAAAAATTAACTCTTAAAAGTGATCAGTTAAATAAACGAATTAAAAATCTGTCTCAAAATGAGGACTTCTATAATAG
CGATGAATATTGTGAAAAAAATAATGAAATTAATACTATTAATGCTAATGACTTTAATAATTTATATAAAGCAAAACGCA
ACAAAATTCTTTTAATTGATGTTAGAGAAAATGAAGAATTTTCTACTTCTGCAATAGAGGGATCTATATCAATTCCCTTA
AGGCATTTGGACCAAAATTCTGACTTAAAATTTATTCAGAAAGAAAGTTTAGGCAAAGAGGTTTTCACTATATGTAAATC
GGGGAAACGTTCTGAAGAAGCATCAAGAATCTTGTCTAAATTCAAAATTCAGTCAAGATCTATTGAAGGCGGCATTGAAA
AGGTAAGAAAAATATTGTGCAACTAA

Upstream 100 bases:

>100_bases
CATTAATAAATCACATTAATACGAGAAATTTAAAAGACTAAAATTTTTATTTTAATTATTATGTTTTTAAAGTTTAGCTT
GCAAAAATTTAAGTATAAAT

Downstream 100 bases:

>100_bases
TAATTTTAAGAATATTTAGTAATCTACACCTCTTTTCAAATCAACTCCAACATTTGCATAATGCTTATGACAAACCATTT
CAGAGTAGACATCAGCAAGA

Product: molybdopterin biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSNDIKFNFLSSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATGIGRIGIVDNDQVEKSNLQRQ
IIHETKTIGNLKIDSAKERIKKLNPNCEILTFPERINPKNALELINKFDVICDCSDNFSTRYLINDSCLILNKPLVFGSV
QGFEGQVSVFNLYKNSPNLRDLLPESPSKNAAPSCAEYGVVGVSTGLIGILQVNEIIKIILKKGETLDGKILIFDLLKMN
IKKLTLKSDQLNKRIKNLSQNEDFYNSDEYCEKNNEINTINANDFNNLYKAKRNKILLIDVRENEEFSTSAIEGSISIPL
RHLDQNSDLKFIQKESLGKEVFTICKSGKRSEEASRILSKFKIQSRSIEGGIEKVRKILCN

Sequences:

>Translated_381_residues
MSNDIKFNFLSSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATGIGRIGIVDNDQVEKSNLQRQ
IIHETKTIGNLKIDSAKERIKKLNPNCEILTFPERINPKNALELINKFDVICDCSDNFSTRYLINDSCLILNKPLVFGSV
QGFEGQVSVFNLYKNSPNLRDLLPESPSKNAAPSCAEYGVVGVSTGLIGILQVNEIIKIILKKGETLDGKILIFDLLKMN
IKKLTLKSDQLNKRIKNLSQNEDFYNSDEYCEKNNEINTINANDFNNLYKAKRNKILLIDVRENEEFSTSAIEGSISIPL
RHLDQNSDLKFIQKESLGKEVFTICKSGKRSEEASRILSKFKIQSRSIEGGIEKVRKILCN
>Mature_380_residues
SNDIKFNFLSSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATGIGRIGIVDNDQVEKSNLQRQI
IHETKTIGNLKIDSAKERIKKLNPNCEILTFPERINPKNALELINKFDVICDCSDNFSTRYLINDSCLILNKPLVFGSVQ
GFEGQVSVFNLYKNSPNLRDLLPESPSKNAAPSCAEYGVVGVSTGLIGILQVNEIIKIILKKGETLDGKILIFDLLKMNI
KKLTLKSDQLNKRIKNLSQNEDFYNSDEYCEKNNEINTINANDFNNLYKAKRNKILLIDVRENEEFSTSAIEGSISIPLR
HLDQNSDLKFIQKESLGKEVFTICKSGKRSEEASRILSKFKIQSRSIEGGIEKVRKILCN

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=395, Percent_Identity=34.4303797468354, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4885649, Length=162, Percent_Identity=30.8641975308642, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI23510338, Length=164, Percent_Identity=26.219512195122, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI23510340, Length=164, Percent_Identity=26.219512195122, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI38045948, Length=166, Percent_Identity=28.3132530120482, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI38045942, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI38045944, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787048, Length=237, Percent_Identity=35.0210970464135, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87082356, Length=233, Percent_Identity=36.9098712446352, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789177, Length=140, Percent_Identity=30, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=394, Percent_Identity=32.2335025380711, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI193203301, Length=135, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321903, Length=390, Percent_Identity=33.5897435897436, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6325438, Length=166, Percent_Identity=30.1204819277108, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322639, Length=194, Percent_Identity=27.8350515463918, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320598, Length=148, Percent_Identity=31.0810810810811, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=380, Percent_Identity=36.0526315789474, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24660640, Length=136, Percent_Identity=32.3529411764706, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28573937, Length=161, Percent_Identity=27.3291925465839, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 42819; Mature: 42687

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDIKFNFLSSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATG
CCCCEEEEEECCCHHHHHHHHCHHHHCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHCC
IGRIGIVDNDQVEKSNLQRQIIHETKTIGNLKIDSAKERIKKLNPNCEILTFPERINPKN
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCEEEECCCCCCHHH
ALELINKFDVICDCSDNFSTRYLINDSCLILNKPLVFGSVQGFEGQVSVFNLYKNSPNLR
HHHHHHHCCEEEECCCCCCEEEEECCCEEEEECCEEEEECCCCCCCEEEEEEECCCCCHH
DLLPESPSKNAAPSCAEYGVVGVSTGLIGILQVNEIIKIILKKGETLDGKILIFDLLKMN
HHCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHH
IKKLTLKSDQLNKRIKNLSQNEDFYNSDEYCEKNNEINTINANDFNNLYKAKRNKILLID
HHHEEECHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHHHHCCCCEEEEE
VRENEEFSTSAIEGSISIPLRHLDQNSDLKFIQKESLGKEVFTICKSGKRSEEASRILSK
ECCCCCCCHHHCCCEEECCHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
FKIQSRSIEGGIEKVRKILCN
HHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNDIKFNFLSSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATG
CCCEEEEEECCCHHHHHHHHCHHHHCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHCC
IGRIGIVDNDQVEKSNLQRQIIHETKTIGNLKIDSAKERIKKLNPNCEILTFPERINPKN
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCEEEECCCCCCHHH
ALELINKFDVICDCSDNFSTRYLINDSCLILNKPLVFGSVQGFEGQVSVFNLYKNSPNLR
HHHHHHHCCEEEECCCCCCEEEEECCCEEEEECCEEEEECCCCCCCEEEEEEECCCCCHH
DLLPESPSKNAAPSCAEYGVVGVSTGLIGILQVNEIIKIILKKGETLDGKILIFDLLKMN
HHCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHH
IKKLTLKSDQLNKRIKNLSQNEDFYNSDEYCEKNNEINTINANDFNNLYKAKRNKILLID
HHHEEECHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHHHHCCCCEEEEE
VRENEEFSTSAIEGSISIPLRHLDQNSDLKFIQKESLGKEVFTICKSGKRSEEASRILSK
ECCCCCCCHHHCCCEEECCHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
FKIQSRSIEGGIEKVRKILCN
HHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]