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The map label for this gene is rlmN [H]

Identifier: 126697010

GI number: 126697010

Start: 1398613

End: 1399656

Strand: Reverse

Name: rlmN [H]

Synonym: P9301_16721

Alternate gene names: 126697010

Gene position: 1399656-1398613 (Counterclockwise)

Preceding gene: 126697011

Following gene: 126697009

Centisome position: 85.25

GC content: 32.38

Gene sequence:

>1044_bases
TTGAAAAATCTTCTTGGGAGTACTATTAAAGATTTAGAAAATGTGGCTTTAGATTATGGTCAGGCTGGTTTTAGAGGTCG
CCAAATCTATAATTGGATTTACAACTATAGAAATAAGAAGAAAAATATTGATCAAATAGAAGTCTTACCTTTAGATTTTA
GGAAGAGGTTAAAAGATGATGGTTTTAAAGTAAGTGATCTAAGCATTCATGAAAGAAACTTAGCAAACGATGGTACTCTA
AAGTTATTACTTTCTACAGAAGATAATGAAAGTATTGAGTGCGTTGGTATACCAACTGAAAAAAGATTAACTGCTTGTCT
CTCAAGTCAAGTTGGTTGCCCAATGGACTGCAAATTTTGTGCAACTGGTAAAGAGGGTTTGAAGAGATCTTTAAAAGTAA
GTGAAATTTTAGATCAAATTTTATTTATCGAAAATGAAATGAATAGAAAAGTCACCAATATTGTTTTCATGGGTATGGGT
GAGCCCTTATTAAATATTGATGACTTGCTCTTATCAATTAGATCTATAAATGAGGATTTAAAAATTAGCCAGAGAAAGAT
AACTGTCAGCACTGTCGCTGTCCCAAAAATGATAAATAAATTATCAGCAAAGTCTTTTAAAATATTAGGCAATTGTCAAT
TTACATTAGCAGTAAGCCTACATGCTCCAAACCAAAAAATAAGAGAAACAATAATACCAAGTGCAAAAAACTATGAGATC
GAGAATATAATTGAAGACTGTAAACAATACGTAAGAGATACTGGCAGGAGGGTAAGTTTTGAATATTTAATGCTAAGAGG
GGTTAACGACAAAATAGAACATGCTAATGAATTAAGTCATTTGCTGAAAGGGTTTCAATGCCACGTAAATTTAATACAGT
ACAACCAAATTGATGAGGTTGAATTTCAAAGAGCATGCCTAAAAGATCTTCAATCATTTCAATCTAGACTCTCTCATAAT
GGCATCGCTGTAAGCTTGCGAAAAAGCAGAGGTCTCGATAAAAATGCTGCATGCGGTCAGTTAAGACAAAATGCAAGAAA
TTAA

Upstream 100 bases:

>100_bases
TGGCCATGATTGGTTTTATTGCGCTTATTCTTACAGAATTCTTTTTAAAACATGGTTTGCTGTTATGGTGAAAATAGTTT
TGAAATAAAGACTACTTAAT

Downstream 100 bases:

>100_bases
TAATATTATCTGGCAGAATATTACCTAAAAAATTTTTTAAAAGTCTCTTTAACAGGTTATCATTGTGTTAATTAATCTTG
AATCTTTGGGTTTTATTAAG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKNLLGSTIKDLENVALDYGQAGFRGRQIYNWIYNYRNKKKNIDQIEVLPLDFRKRLKDDGFKVSDLSIHERNLANDGTL
KLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKVSEILDQILFIENEMNRKVTNIVFMGMG
EPLLNIDDLLLSIRSINEDLKISQRKITVSTVAVPKMINKLSAKSFKILGNCQFTLAVSLHAPNQKIRETIIPSAKNYEI
ENIIEDCKQYVRDTGRRVSFEYLMLRGVNDKIEHANELSHLLKGFQCHVNLIQYNQIDEVEFQRACLKDLQSFQSRLSHN
GIAVSLRKSRGLDKNAACGQLRQNARN

Sequences:

>Translated_347_residues
MKNLLGSTIKDLENVALDYGQAGFRGRQIYNWIYNYRNKKKNIDQIEVLPLDFRKRLKDDGFKVSDLSIHERNLANDGTL
KLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKVSEILDQILFIENEMNRKVTNIVFMGMG
EPLLNIDDLLLSIRSINEDLKISQRKITVSTVAVPKMINKLSAKSFKILGNCQFTLAVSLHAPNQKIRETIIPSAKNYEI
ENIIEDCKQYVRDTGRRVSFEYLMLRGVNDKIEHANELSHLLKGFQCHVNLIQYNQIDEVEFQRACLKDLQSFQSRLSHN
GIAVSLRKSRGLDKNAACGQLRQNARN
>Mature_347_residues
MKNLLGSTIKDLENVALDYGQAGFRGRQIYNWIYNYRNKKKNIDQIEVLPLDFRKRLKDDGFKVSDLSIHERNLANDGTL
KLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKVSEILDQILFIENEMNRKVTNIVFMGMG
EPLLNIDDLLLSIRSINEDLKISQRKITVSTVAVPKMINKLSAKSFKILGNCQFTLAVSLHAPNQKIRETIIPSAKNYEI
ENIIEDCKQYVRDTGRRVSFEYLMLRGVNDKIEHANELSHLLKGFQCHVNLIQYNQIDEVEFQRACLKDLQSFQSRLSHN
GIAVSLRKSRGLDKNAACGQLRQNARN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=351, Percent_Identity=35.042735042735, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39513; Mature: 39513

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLGSTIKDLENVALDYGQAGFRGRQIYNWIYNYRNKKKNIDQIEVLPLDFRKRLKDD
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHCCC
GFKVSDLSIHERNLANDGTLKLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFC
CCEEEECCCHHCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKVSEILDQILFIENEMNRKVTNIVFMGMGEPLLNIDDLLLSIRSINEDL
CCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCC
KISQRKITVSTVAVPKMINKLSAKSFKILGNCQFTLAVSLHAPNQKIRETIIPSAKNYEI
EEHHHEEEEHHHHHHHHHHHHCCCCEEEEECEEEEEEEEEECCCHHHHHHHCCCCCCCCH
ENIIEDCKQYVRDTGRRVSFEYLMLRGVNDKIEHANELSHLLKGFQCHVNLIQYNQIDEV
HHHHHHHHHHHHHCCCEEEEEEHEEECCCHHHHHHHHHHHHHCCHHEEEEEEECCCCHHH
EFQRACLKDLQSFQSRLSHNGIAVSLRKSRGLDKNAACGQLRQNARN
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKNLLGSTIKDLENVALDYGQAGFRGRQIYNWIYNYRNKKKNIDQIEVLPLDFRKRLKDD
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHCCC
GFKVSDLSIHERNLANDGTLKLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFC
CCEEEECCCHHCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKVSEILDQILFIENEMNRKVTNIVFMGMGEPLLNIDDLLLSIRSINEDL
CCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCC
KISQRKITVSTVAVPKMINKLSAKSFKILGNCQFTLAVSLHAPNQKIRETIIPSAKNYEI
EEHHHEEEEHHHHHHHHHHHHCCCCEEEEECEEEEEEEEEECCCHHHHHHHCCCCCCCCH
ENIIEDCKQYVRDTGRRVSFEYLMLRGVNDKIEHANELSHLLKGFQCHVNLIQYNQIDEV
HHHHHHHHHHHHHCCCEEEEEEHEEECCCHHHHHHHHHHHHHCCHHEEEEEEECCCCHHH
EFQRACLKDLQSFQSRLSHNGIAVSLRKSRGLDKNAACGQLRQNARN
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA