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The map label for this gene is hisF2 [H]

Identifier: 126696768

GI number: 126696768

Start: 1198291

End: 1198959

Strand: Direct

Name: hisF2 [H]

Synonym: P9301_14301

Alternate gene names: 126696768

Gene position: 1198291-1198959 (Clockwise)

Preceding gene: 126696767

Following gene: 126696769

Centisome position: 72.98

GC content: 29.45

Gene sequence:

>669_bases
ATGATAAAGACTTTTGGTTTTTCTAATATTGCTAATTGCTCTGATGAGATTCTTATAATAAATCTCTCTGAAAAAAATTG
GGAAGAGGCATACAATAACAAATTTTTCAATGATATATCTCTACTTTTATCAAAAACGTTCTTACCAATTACTATTGGGG
GGAAAATAAGAAGTATAGAGAATGCAAAAGAACTCTTCAATTTTGGCGCCGATAAAATACTAATATCAAGAGCTTTTTTT
CAAGATCAAAATTTATTAAAAGAAATACGAGATTTATATGGAGTTCAGTCAATTGTGGTTCAGTTTGACATAAATTCCCA
AAATTATGATATTTTCATTAATTCTTCTTTAGAAAAAGTTTCTAATTTGAAGAAAATAGTTTATTCATTAAATGAAAAGA
ATCTTATGGGTGAACTAATCATTAATCAAATTGACAGAGACGGTACAGGTTTTGGACATAATACAGAATATTTAAATGCA
ATTCCAAAATTGAATATGCCATTAATTGCTAGTGGAGGATTTGGTTCTTCCATACATATGGCCGAATGTCTCAAATTTGA
TTCAATTAGCGCAGTAGCGACATCTAACCTCTTAAATTTTATTGGAGATGCTTTTTCAGAAGCTCGTCAAGAGCTAAGGA
AAATGAATTTTGAATTAACGCCTTGGTAG

Upstream 100 bases:

>100_bases
ATAAATGATTAAACGCACTTTTTTCACTTTACTTTATAAAGAAGGCTTTTTCTTTCTTAGTAGGAATTTTAGATTACAAA
AAGTAGGAGATATAAATTGG

Downstream 100 bases:

>100_bases
AATTATTTTAGTTGATTTTTAATTATGGTTTTTTGGTGTAAAAGCTGTTTAAATATGTCCACAAGACCTCGAATTGAATT
CAATTCCGATTCAATATGTA

Product: hypothetical protein

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF2; ImGP synthase subunit hisF2; IGPS subunit hisF2 [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MIKTFGFSNIANCSDEILIINLSEKNWEEAYNNKFFNDISLLLSKTFLPITIGGKIRSIENAKELFNFGADKILISRAFF
QDQNLLKEIRDLYGVQSIVVQFDINSQNYDIFINSSLEKVSNLKKIVYSLNEKNLMGELIINQIDRDGTGFGHNTEYLNA
IPKLNMPLIASGGFGSSIHMAECLKFDSISAVATSNLLNFIGDAFSEARQELRKMNFELTPW

Sequences:

>Translated_222_residues
MIKTFGFSNIANCSDEILIINLSEKNWEEAYNNKFFNDISLLLSKTFLPITIGGKIRSIENAKELFNFGADKILISRAFF
QDQNLLKEIRDLYGVQSIVVQFDINSQNYDIFINSSLEKVSNLKKIVYSLNEKNLMGELIINQIDRDGTGFGHNTEYLNA
IPKLNMPLIASGGFGSSIHMAECLKFDSISAVATSNLLNFIGDAFSEARQELRKMNFELTPW
>Mature_222_residues
MIKTFGFSNIANCSDEILIINLSEKNWEEAYNNKFFNDISLLLSKTFLPITIGGKIRSIENAKELFNFGADKILISRAFF
QDQNLLKEIRDLYGVQSIVVQFDINSQNYDIFINSSLEKVSNLKKIVYSLNEKNLMGELIINQIDRDGTGFGHNTEYLNA
IPKLNMPLIASGGFGSSIHMAECLKFDSISAVATSNLLNFIGDAFSEARQELRKMNFELTPW

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=201, Percent_Identity=28.3582089552239, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR020021
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 25114; Mature: 25114

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTFGFSNIANCSDEILIINLSEKNWEEAYNNKFFNDISLLLSKTFLPITIGGKIRSIE
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCCHH
NAKELFNFGADKILISRAFFQDQNLLKEIRDLYGVQSIVVQFDINSQNYDIFINSSLEKV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEECCCHHHH
SNLKKIVYSLNEKNLMGELIINQIDRDGTGFGHNTEYLNAIPKLNMPLIASGGFGSSIHM
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCHHH
AECLKFDSISAVATSNLLNFIGDAFSEARQELRKMNFELTPW
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIKTFGFSNIANCSDEILIINLSEKNWEEAYNNKFFNDISLLLSKTFLPITIGGKIRSIE
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCCHH
NAKELFNFGADKILISRAFFQDQNLLKEIRDLYGVQSIVVQFDINSQNYDIFINSSLEKV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEECCCHHHH
SNLKKIVYSLNEKNLMGELIINQIDRDGTGFGHNTEYLNAIPKLNMPLIASGGFGSSIHM
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCHHH
AECLKFDSISAVATSNLLNFIGDAFSEARQELRKMNFELTPW
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]