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The map label for this gene is hisH [H]

Identifier: 126696767

GI number: 126696767

Start: 1197577

End: 1198194

Strand: Direct

Name: hisH [H]

Synonym: P9301_14291

Alternate gene names: 126696767

Gene position: 1197577-1198194 (Clockwise)

Preceding gene: 126696766

Following gene: 126696768

Centisome position: 72.94

GC content: 30.26

Gene sequence:

>618_bases
ATGAATCCTAAGGTTGGAATAATTGACTATAACTGTGGCAATTTAGGCTCCATAACAAAAGCTTTTTCATTTATTGGCTG
CGAAACGAAAATCCTAAGAGATAATGATAAAAATAATTGTTCTCATCTTGTTTTGCCAGGAGTCGGATCATTTAAAAAAG
GATCTGACTATATCTTTAAAATATCTAAAAGTATAGAAGAAATAACATTATCTCACTTTGCAATGGAGAGGCCTCTACTG
GGTATATGTTTAGGTTACCAACTTTTTTCAAAAGGAGGAACAGAAGGGGGGCAATCAAAAGGATTCGGTTTTTTAGATGC
GGATTGCGAACATTTAAGCAAAATAATTGATCCAAATGAAATAAAAATACCAAATGTAGGATTTCAAAAATTATCTTATG
ATTCTTCAGAACTAAACTTAAAAGATAATTGCTATTATTTTGTTCATAGTTATGCTTTAAGATTTAAAAACCCACCCCAA
AATGTTCAAAAGATAATTATTGGGGGAGAAGAATTAATTATAGGTTTTCAACATAAAAACCTCTGGGGCTTTCAGTTTCA
TCCAGAAAAAAGTCAACTAAAAGGTTTGGATTTACTTAGAAAATTTATTTCTTTATAA

Upstream 100 bases:

>100_bases
GTAAAACTTAATTATTTTTATTTAATAGATTAAAAATTTCGTTTTAGCATAATCTTTTTAATAAGTTAATGTTAAACTTC
TAACCATACAATTATTTGAG

Downstream 100 bases:

>100_bases
ATGATTAAACGCACTTTTTTCACTTTACTTTATAAAGAAGGCTTTTTCTTTCTTAGTAGGAATTTTAGATTACAAAAAGT
AGGAGATATAAATTGGATGA

Product: glutamine amidotransferase

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MNPKVGIIDYNCGNLGSITKAFSFIGCETKILRDNDKNNCSHLVLPGVGSFKKGSDYIFKISKSIEEITLSHFAMERPLL
GICLGYQLFSKGGTEGGQSKGFGFLDADCEHLSKIIDPNEIKIPNVGFQKLSYDSSELNLKDNCYYFVHSYALRFKNPPQ
NVQKIIIGGEELIIGFQHKNLWGFQFHPEKSQLKGLDLLRKFISL

Sequences:

>Translated_205_residues
MNPKVGIIDYNCGNLGSITKAFSFIGCETKILRDNDKNNCSHLVLPGVGSFKKGSDYIFKISKSIEEITLSHFAMERPLL
GICLGYQLFSKGGTEGGQSKGFGFLDADCEHLSKIIDPNEIKIPNVGFQKLSYDSSELNLKDNCYYFVHSYALRFKNPPQ
NVQKIIIGGEELIIGFQHKNLWGFQFHPEKSQLKGLDLLRKFISL
>Mature_205_residues
MNPKVGIIDYNCGNLGSITKAFSFIGCETKILRDNDKNNCSHLVLPGVGSFKKGSDYIFKISKSIEEITLSHFAMERPLL
GICLGYQLFSKGGTEGGQSKGFGFLDADCEHLSKIIDPNEIKIPNVGFQKLSYDSSELNLKDNCYYFVHSYALRFKNPPQ
NVQKIIIGGEELIIGFQHKNLWGFQFHPEKSQLKGLDLLRKFISL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=206, Percent_Identity=31.5533980582524, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=30.3738317757009, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23018; Mature: 23018

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKVGIIDYNCGNLGSITKAFSFIGCETKILRDNDKNNCSHLVLPGVGSFKKGSDYIFK
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCEEE
ISKSIEEITLSHFAMERPLLGICLGYQLFSKGGTEGGQSKGFGFLDADCEHLSKIIDPNE
EHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
IKIPNVGFQKLSYDSSELNLKDNCYYFVHSYALRFKNPPQNVQKIIIGGEELIIGFQHKN
EECCCCCHHHCCCCCCCCCCCCCEEEEEEEEHHCCCCCHHHHHEEEECCCEEEEEEECCC
LWGFQFHPEKSQLKGLDLLRKFISL
EECEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPKVGIIDYNCGNLGSITKAFSFIGCETKILRDNDKNNCSHLVLPGVGSFKKGSDYIFK
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCEEE
ISKSIEEITLSHFAMERPLLGICLGYQLFSKGGTEGGQSKGFGFLDADCEHLSKIIDPNE
EHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
IKIPNVGFQKLSYDSSELNLKDNCYYFVHSYALRFKNPPQNVQKIIIGGEELIIGFQHKN
EECCCCCHHHCCCCCCCCCCCCCEEEEEEEEHHCCCCCHHHHHEEEECCCEEEEEEECCC
LWGFQFHPEKSQLKGLDLLRKFISL
EECEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA