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The map label for this gene is phoB [H]

Identifier: 126696558

GI number: 126696558

Start: 1035776

End: 1036513

Strand: Direct

Name: phoB [H]

Synonym: P9301_12201

Alternate gene names: 126696558

Gene position: 1035776-1036513 (Clockwise)

Preceding gene: 126696556

Following gene: 126696563

Centisome position: 63.08

GC content: 44.72

Gene sequence:

>738_bases
ATGCAATCAACTGAGCAAATCTTAGCTTCAACTCCTGGCAGTTCACAATTGCCTCAGAGCTCTCAAACCCCCTCAAGAGT
TTTAGTTGTTGAACCTCACCCCACACTTAGAACGGTCCTTGTTCAAAGGCTTCGCCAAGATGGCCATTTAGCTGCGGCAG
TAGGCACAGCAGCTGAAGCTATTGACTTATGCAGAGAACAATCACCTGACCTATTAGTTAGCGCAGAAATACTCGATCAG
AATACTGCAATGAGACTCGCCCAACAATTAGGGTCCTCTGTCATAGTTTTAACGGCAAGATCAGGTGTGGAAGCATTAGT
TAATTTGTTAGATGAAGGTGCAGATGATGTTCTTAGAAAACCATTTGGTCTAGAAGAGCTTGCAGCTAGATGTAGAACCC
TTTTGAAAAGAGGGAGAATTGGATTACAAGAAAAAGTTGAGGTTGGCCCACTAGAGGTTCATCTTCTTTTAAGACAGGTA
ACTCTTAGCGAGAAGCCCGTAGAATTAAGCCCTAGAGAGTTTGCACTCCTTTGCGCCCTTTTAATGCCACCTGGCATGGT
TAGGAGCCGACAAGAGCTTCTGAGAATGGCCTGGCCACCCTTCAGCGGGGGGCCGAGATCTGTAGATACTCAGGTATTAA
CTTTGCGGAGAAAATTAGAACAGGCAGGCTTGGGAGAAGGTGGTGGTATAACCACTGTTAGACAACAAGGTTATAGATTC
AGTATTGATAATATTTAG

Upstream 100 bases:

>100_bases
TCAAATGATGTCATAATTCGATAGATTCTGATTTTTATTTAGCATTTGTTATACCTTATTAAGGATGACCAAATATTGCC
CGGCCAAACAGTAATTAAGA

Downstream 100 bases:

>100_bases
CTTAGAAAAGCAAAAATCTCCCCCGCAATCATCATTATCGAAAGTAAAGTCAATAACTTATAAGTCCATAGTGGTGATAT
GTAAGATATTTCATTAATAT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MQSTEQILASTPGSSQLPQSSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGTAAEAIDLCREQSPDLLVSAEILDQ
NTAMRLAQQLGSSVIVLTARSGVEALVNLLDEGADDVLRKPFGLEELAARCRTLLKRGRIGLQEKVEVGPLEVHLLLRQV
TLSEKPVELSPREFALLCALLMPPGMVRSRQELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
SIDNI

Sequences:

>Translated_245_residues
MQSTEQILASTPGSSQLPQSSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGTAAEAIDLCREQSPDLLVSAEILDQ
NTAMRLAQQLGSSVIVLTARSGVEALVNLLDEGADDVLRKPFGLEELAARCRTLLKRGRIGLQEKVEVGPLEVHLLLRQV
TLSEKPVELSPREFALLCALLMPPGMVRSRQELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
SIDNI
>Mature_245_residues
MQSTEQILASTPGSSQLPQSSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGTAAEAIDLCREQSPDLLVSAEILDQ
NTAMRLAQQLGSSVIVLTARSGVEALVNLLDEGADDVLRKPFGLEELAARCRTLLKRGRIGLQEKVEVGPLEVHLLLRQV
TLSEKPVELSPREFALLCALLMPPGMVRSRQELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
SIDNI

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=31.5555555555556, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788394, Length=236, Percent_Identity=29.6610169491525, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=29.5964125560538, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=33.9366515837104, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=29.9107142857143, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=32.4561403508772, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790552, Length=147, Percent_Identity=36.0544217687075, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=30.2127659574468, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787229, Length=209, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI2367329, Length=173, Percent_Identity=34.1040462427746, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26693; Mature: 26693

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTEQILASTPGSSQLPQSSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGTAAEA
CCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHH
IDLCREQSPDLLVSAEILDQNTAMRLAQQLGSSVIVLTARSGVEALVNLLDEGADDVLRK
HHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCHHHHHHC
PFGLEELAARCRTLLKRGRIGLQEKVEVGPLEVHLLLRQVTLSEKPVELSPREFALLCAL
CCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LMPPGMVRSRQELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
HCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEE
SIDNI
ECCCC
>Mature Secondary Structure
MQSTEQILASTPGSSQLPQSSQTPSRVLVVEPHPTLRTVLVQRLRQDGHLAAAVGTAAEA
CCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHH
IDLCREQSPDLLVSAEILDQNTAMRLAQQLGSSVIVLTARSGVEALVNLLDEGADDVLRK
HHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCHHHHHHC
PFGLEELAARCRTLLKRGRIGLQEKVEVGPLEVHLLLRQVTLSEKPVELSPREFALLCAL
CCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LMPPGMVRSRQELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGEGGGITTVRQQGYRF
HCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEE
SIDNI
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]