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The map label for this gene is dus1 [H]

Identifier: 126696436

GI number: 126696436

Start: 936647

End: 937654

Strand: Direct

Name: dus1 [H]

Synonym: P9301_10981

Alternate gene names: 126696436

Gene position: 936647-937654 (Clockwise)

Preceding gene: 126696433

Following gene: 126696437

Centisome position: 57.05

GC content: 32.14

Gene sequence:

>1008_bases
ATGTCTTCAAATATAAAGCTAAAAGGAAGGGGGGTTAACAGAATAATTAAGAGTAAGGTCATGCTATCGCCATTAGCAGG
AGTTACAGATATCATTTTTAGGCGACTTGTACGTAAATGGGCTCCAAACTCTTTACTTTTTACAGAAATGATAAATGCCA
CAAGTCTTAAAAAAGGATATGGCACACAAAAAATCAATCAAATAGATTTAGAAGAAGGTCCAATTGGAGTTCAAATATTT
GATAATAGGCCATATGCTGTTTCTGAAGCAGCAAAACAAGCTGAGGACTCTGGAGCTTTCTTAATTGATATAAATATGGG
ATGTCCAGTAAAAAAAATTGCAAAGAAAGGTGGAGGCAGTGCTTTAATTAAAGACCGAAAACTTGCTATAGAATTAGTCA
AAAATGTTGTAAAAGCTGTTAGGGTTCCAGTAACTGTAAAAACACGACTCGGATGGGATAGTAAAGAAGAAAATATAGAG
GATTTCCTATTTAAACTTCAAGATGCGGGAGCAACGATGATCACACTTCATGGAAGAACTAGAAAACAAGGTTTTTCAGG
CAAGTCAGATTGGGAAATGATCGGGAGACTTAAAAAGTTGTTGGAAATTCCAGTAATTGCTAATGGAGATATAAAAAATC
CAGATGACGCTCTTAATTGTTTAAAAAAAACAAATGCTGATGGTGTAATGATTGGACGAGGAATTTTAGGATCCCCATGG
AAAATAGGAGAAATAGATTATGCCCTTAGAGAAAATAAAAATTTTAAAGAACCAAACATAGAAGAAAAACTATATTTAAT
TATTGAGCATCTTGATGAATTAGTAAAAGAAAAAGGAGATCACGGATTGCTAATTGCAAGGAAACATATTTCATGGACGT
GCAAAGACTTTAAAGGAGCATCAAATTTGAGAAATAACTTGGTTAGAGCTGTTGATAAAAATGAAGTTAAAAATTTAATA
AATAAAATGATTAAAACTTTGAATAATAAAAAAAATATATTAGCTTAA

Upstream 100 bases:

>100_bases
TAAAAGCACTTAATTATAAATTAAGAAATAAATAGAAAACATAAAAACAGCTCCATATATGAATTTATAATAAAGGAAAA
TTAAATAAATTTAAAATTAT

Downstream 100 bases:

>100_bases
AACATATTTATTTTTTAAATGAAAATTATTAGCAAAGAGACAAGTAAAAGAGTTTGTGATCACATGAACAATGATCACAT
AGATTCAGTGCACAAATATC

Product: nitrogen regulation protein NifR3 family-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSSNIKLKGRGVNRIIKSKVMLSPLAGVTDIIFRRLVRKWAPNSLLFTEMINATSLKKGYGTQKINQIDLEEGPIGVQIF
DNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIE
DFLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTNADGVMIGRGILGSPW
KIGEIDYALRENKNFKEPNIEEKLYLIIEHLDELVKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLI
NKMIKTLNNKKNILA

Sequences:

>Translated_335_residues
MSSNIKLKGRGVNRIIKSKVMLSPLAGVTDIIFRRLVRKWAPNSLLFTEMINATSLKKGYGTQKINQIDLEEGPIGVQIF
DNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIE
DFLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTNADGVMIGRGILGSPW
KIGEIDYALRENKNFKEPNIEEKLYLIIEHLDELVKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLI
NKMIKTLNNKKNILA
>Mature_334_residues
SSNIKLKGRGVNRIIKSKVMLSPLAGVTDIIFRRLVRKWAPNSLLFTEMINATSLKKGYGTQKINQIDLEEGPIGVQIFD
NRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIED
FLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTNADGVMIGRGILGSPWK
IGEIDYALRENKNFKEPNIEEKLYLIIEHLDELVKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLIN
KMIKTLNNKKNILA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=236, Percent_Identity=31.7796610169492, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=31.7796610169492, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=31.140350877193, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=34.070796460177, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI8923374, Length=142, Percent_Identity=33.0985915492958, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789660, Length=302, Percent_Identity=34.7682119205298, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788462, Length=304, Percent_Identity=32.2368421052632, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI145693211, Length=242, Percent_Identity=29.7520661157025, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=34.5991561181435, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=31.3084112149533, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=28.75, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=282, Percent_Identity=31.5602836879433, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=257, Percent_Identity=28.0155642023346, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=29.646017699115, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585320, Length=238, Percent_Identity=34.0336134453782, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=31.1111111111111, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=31.1111111111111, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19921524, Length=235, Percent_Identity=32.7659574468085, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45549423, Length=236, Percent_Identity=26.271186440678, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37422; Mature: 37291

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNIKLKGRGVNRIIKSKVMLSPLAGVTDIIFRRLVRKWAPNSLLFTEMINATSLKKGY
CCCCEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
GTQKINQIDLEEGPIGVQIFDNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGS
CCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCC
ALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIEDFLFKLQDAGATMITLHGRT
EEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCH
RKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTNADGVMIGRGILGSPW
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCE
KIGEIDYALRENKNFKEPNIEEKLYLIIEHLDELVKEKGDHGLLIARKHISWTCKDFKGA
ECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHCCH
SNLRNNLVRAVDKNEVKNLINKMIKTLNNKKNILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSNIKLKGRGVNRIIKSKVMLSPLAGVTDIIFRRLVRKWAPNSLLFTEMINATSLKKGY
CCCEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
GTQKINQIDLEEGPIGVQIFDNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGS
CCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCC
ALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIEDFLFKLQDAGATMITLHGRT
EEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCH
RKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTNADGVMIGRGILGSPW
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCE
KIGEIDYALRENKNFKEPNIEEKLYLIIEHLDELVKEKGDHGLLIARKHISWTCKDFKGA
ECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHCCH
SNLRNNLVRAVDKNEVKNLINKMIKTLNNKKNILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]