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The map label for this gene is vacB [H]

Identifier: 126696359

GI number: 126696359

Start: 879468

End: 881729

Strand: Direct

Name: vacB [H]

Synonym: P9301_10211

Alternate gene names: 126696359

Gene position: 879468-881729 (Clockwise)

Preceding gene: 126696358

Following gene: 126696360

Centisome position: 53.56

GC content: 27.06

Gene sequence:

>2262_bases
ATGTATACACCTAAATTAATTTATAATTTCATTCTGATCATGTTCACATCTTCATCAATTATTGATAATCTTAATCAGTC
AGAAGGGTTAGAATATAAAAAATTATGCAGATTGTTAAAAATAACAAAGAAATCTGATAAGGATAAATTAGATATTGCTT
TAACAGCTTTAGAAAAACTTGAAATAATTAATAAAAATGAAAGTGATGAATATACCTGCATAAAAGATAGTGATCATCTT
GTCGCCAAAATAAGATGTAGTAGCAAAGGCTATTGCTTTGCTGTAAGAGGAAAAGACAAAGAAGATATCTACATAAAAGA
AAATCTACTTAACTATGCGTGGAATGGAGATAAAGTATTAGTAAGGATAATAAAAGAGGGTTATAGAAGAAGATCACCTG
AGGGAATAGTTGATTGTATTCTTGAAAGATCAAATCAAATACTTCTTTCTAAGGTTGAAATAATAAACAATGATGTATAT
GCAATCCCAATAGACGATAGAATCCTTTCTAAAATTAAACTTCCAAAAGAGAATAAAAAATACATTTTCAATCCAGACAA
TAAGAATATAGTAAAAGTTGAGATTGATAGATTTCCGATTGGTCAAGAAGAAGGACTAGGTCATGTCATACAAGAACTAA
CACTAAACAATAATGAGGAATATGACTCAGACTTTGTTTTGTCTAAAAGTAATATCGCAAAATCATACAATTTAAATCAT
ATTGAATCAAAAAAAATAGAAAAAAGGGAAAGGATAGACCTTACAGACAAAAACTCTTATTTATTCAAAAGTTGGAGTTC
TAATAATTCTCCAATGCTCCCAATGATTCAAATTGAGCAGGTAAAAAATAATAATACTAAATTGTGGATACATACAAATA
ATCTTGCAGAAAGAGTAGATCTAAATAGTAAAAAATCTCAAGAAATACTATTAAAACGTTTTGAATCATTACCCTTATTA
AATGATTGGGAAAACTACCTTAGTGAAGCCATAAGAAATGATTCTGAATTTAAATTAGGTAAAAAGAGTGAAGCGATAAG
CCTCTGTGTGCATTTAAATAGTGATAATGAAATAATTGATTGGTCGTTTCATCTTACTTTAGTCAAGTGCTCTCTTATTG
TTGGAAGTGATCATACTGACGCTCTTCTATCTAGAAAAAGTAAATCAAGAATAACCTCTCGAGTATTAAAACCTATAAAG
GAATATATTGAAGATTTAGATAAAATACTGGAAATTTCATTTTCATTTAGACAAAAACATCTTTTGGAGGGTAAGGTTGA
AATTCCTGCGCCAGTCAACAAAATAAAAGCAATAGAAGAATTTTTTATTCACAATCCAGCTGAATATTCAAAGGGATATT
TTGAATCATTAAATAAAGAAGATTGCCAAACTTTTCTTTCACCAATACTATATGAGGCTAATTTAATATGGTTCAAACAT
TCAAATAAATATGGCTTAAAAAGTGCAGGATACATCTCAAAGGGAATAGATTACGTTAATGCAAATGAAATCATCAAATA
TTCAGAATTTATTGATAATGATATGGAGCTTAATGAAGATGGAAATTTGACATTTAGTCAAGTAATTAAAATATGTGGCG
ATGATAATAAAAAAAGAATCTTACATAAACTTCTAATTAATGAATTTGAGGACAACGAAATAAGGTTGATTTCAAAAAAT
CCTGATAATGACGAATCAGAAAAATTATTTATTTCTCCATGGACAATTCCTAGTTTTGACTTCACTAACCTTATAAACCA
GTACTGTATTTTTAATATGATAATAAATGGTAAGAAATCAAAGAAAAACAATATTAATGAAATCAATATATCGGAAAGTA
ACTCATTAGACTTAGTAAATTGGGATATATTTAATTCATCAATTTCAAAGAATCTAGAAGCATTATTTAATAAGTTGTTA
ATAGATAAACATAATGAATTCAAGTACAAAGTTAACCAATATAAATCTAATATGATTAGTATAAAAAAAGTAAGAAAAGC
AGAAAAGTTACTAGGTAATACTTATAGTGGATTTATATTATCAGTACAAACATATGGTTTCTTTGTTGAGATATCAGAAC
TAAATGTAGAGGGTTTAGTACACGTCAGCACTCTTAATAATGATTGGTATGAATATAGATCAAGGCAAAATCTATTAATT
GGAAGAAAATCTAAAAAATCATATAAAGTTGGAGATGCAATAGAAGTAAAAATCATAAAAGTCGATATTCTTAAATATCA
AATTGATTTAGAATTAACTTAA

Upstream 100 bases:

>100_bases
ATAAAGCTTTTTATCAACTCTTTCACCATCGAAAAAGAGGAAAAAGAAGAGAATAAAAAATAATTAGTTTTTAGCTTGGA
TAATAAGGTGTAATAATGAA

Downstream 100 bases:

>100_bases
ATCAATTCTCACAAAATATATTATGGAAATATTAACCAAAAAAATTTAAGATAAATTTTTAGAATTATGTTTAAGAAAAA
ATATTTATTATTAACTCTTT

Product: putative acetazolamide conferring resistance protein Zam

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 753; Mature: 753

Protein sequence:

>753_residues
MYTPKLIYNFILIMFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNESDEYTCIKDSDHL
VAKIRCSSKGYCFAVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVY
AIPIDDRILSKIKLPKENKKYIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELTLNNNEEYDSDFVLSKSNIAKSYNLNH
IESKKIEKRERIDLTDKNSYLFKSWSSNNSPMLPMIQIEQVKNNNTKLWIHTNNLAERVDLNSKKSQEILLKRFESLPLL
NDWENYLSEAIRNDSEFKLGKKSEAISLCVHLNSDNEIIDWSFHLTLVKCSLIVGSDHTDALLSRKSKSRITSRVLKPIK
EYIEDLDKILEISFSFRQKHLLEGKVEIPAPVNKIKAIEEFFIHNPAEYSKGYFESLNKEDCQTFLSPILYEANLIWFKH
SNKYGLKSAGYISKGIDYVNANEIIKYSEFIDNDMELNEDGNLTFSQVIKICGDDNKKRILHKLLINEFEDNEIRLISKN
PDNDESEKLFISPWTIPSFDFTNLINQYCIFNMIINGKKSKKNNINEINISESNSLDLVNWDIFNSSISKNLEALFNKLL
IDKHNEFKYKVNQYKSNMISIKKVRKAEKLLGNTYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLI
GRKSKKSYKVGDAIEVKIIKVDILKYQIDLELT

Sequences:

>Translated_753_residues
MYTPKLIYNFILIMFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNESDEYTCIKDSDHL
VAKIRCSSKGYCFAVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVY
AIPIDDRILSKIKLPKENKKYIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELTLNNNEEYDSDFVLSKSNIAKSYNLNH
IESKKIEKRERIDLTDKNSYLFKSWSSNNSPMLPMIQIEQVKNNNTKLWIHTNNLAERVDLNSKKSQEILLKRFESLPLL
NDWENYLSEAIRNDSEFKLGKKSEAISLCVHLNSDNEIIDWSFHLTLVKCSLIVGSDHTDALLSRKSKSRITSRVLKPIK
EYIEDLDKILEISFSFRQKHLLEGKVEIPAPVNKIKAIEEFFIHNPAEYSKGYFESLNKEDCQTFLSPILYEANLIWFKH
SNKYGLKSAGYISKGIDYVNANEIIKYSEFIDNDMELNEDGNLTFSQVIKICGDDNKKRILHKLLINEFEDNEIRLISKN
PDNDESEKLFISPWTIPSFDFTNLINQYCIFNMIINGKKSKKNNINEINISESNSLDLVNWDIFNSSISKNLEALFNKLL
IDKHNEFKYKVNQYKSNMISIKKVRKAEKLLGNTYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLI
GRKSKKSYKVGDAIEVKIIKVDILKYQIDLELT
>Mature_753_residues
MYTPKLIYNFILIMFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNESDEYTCIKDSDHL
VAKIRCSSKGYCFAVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVY
AIPIDDRILSKIKLPKENKKYIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELTLNNNEEYDSDFVLSKSNIAKSYNLNH
IESKKIEKRERIDLTDKNSYLFKSWSSNNSPMLPMIQIEQVKNNNTKLWIHTNNLAERVDLNSKKSQEILLKRFESLPLL
NDWENYLSEAIRNDSEFKLGKKSEAISLCVHLNSDNEIIDWSFHLTLVKCSLIVGSDHTDALLSRKSKSRITSRVLKPIK
EYIEDLDKILEISFSFRQKHLLEGKVEIPAPVNKIKAIEEFFIHNPAEYSKGYFESLNKEDCQTFLSPILYEANLIWFKH
SNKYGLKSAGYISKGIDYVNANEIIKYSEFIDNDMELNEDGNLTFSQVIKICGDDNKKRILHKLLINEFEDNEIRLISKN
PDNDESEKLFISPWTIPSFDFTNLINQYCIFNMIINGKKSKKNNINEINISESNSLDLVNWDIFNSSISKNLEALFNKLL
IDKHNEFKYKVNQYKSNMISIKKVRKAEKLLGNTYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLI
GRKSKKSYKVGDAIEVKIIKVDILKYQIDLELT

Specific function: Not known; control resistance to the carbonic anhydrase inhibitor acetazolamide [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082383, Length=82, Percent_Identity=40.2439024390244, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 87537; Mature: 87537

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50126 S1 ; PS00591 GLYCOSYL_HYDROL_F10

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTPKLIYNFILIMFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKL
CCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EIINKNESDEYTCIKDSDHLVAKIRCSSKGYCFAVRGKDKEDIYIKENLLNYAWNGDKVL
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCEEEECCCCCCCEEEEEHHHHHCCCCCHHH
VRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVYAIPIDDRILSKIKLPKENKK
HHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHCCCEEEEECCHHHHHHCCCCCCCCC
YIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELTLNNNEEYDSDFVLSKSNIAKSYNLNH
EEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
IESKKIEKRERIDLTDKNSYLFKSWSSNNSPMLPMIQIEQVKNNNTKLWIHTNNLAERVD
CCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEEEECCHHHHCC
LNSKKSQEILLKRFESLPLLNDWENYLSEAIRNDSEFKLGKKSEAISLCVHLNSDNEIID
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEE
WSFHLTLVKCSLIVGSDHTDALLSRKSKSRITSRVLKPIKEYIEDLDKILEISFSFRQKH
EEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEGKVEIPAPVNKIKAIEEFFIHNPAEYSKGYFESLNKEDCQTFLSPILYEANLIWFKH
HHCCCEECCCCHHHHHHHHHHHCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHCEEEEEEE
SNKYGLKSAGYISKGIDYVNANEIIKYSEFIDNDMELNEDGNLTFSQVIKICGDDNKKRI
CCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHCCCCHHHH
LHKLLINEFEDNEIRLISKNPDNDESEKLFISPWTIPSFDFTNLINQYCIFNMIINGKKS
HHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCC
KKNNINEINISESNSLDLVNWDIFNSSISKNLEALFNKLLIDKHNEFKYKVNQYKSNMIS
CCCCCCEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
IKKVRKAEKLLGNTYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLI
HHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEEECCCHHHHHHCCCCEEE
GRKSKKSYKVGDAIEVKIIKVDILKYQIDLELT
ECCCCCCCCCCCEEEEEEEEEEEEEEEEEEEEC
>Mature Secondary Structure
MYTPKLIYNFILIMFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKL
CCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EIINKNESDEYTCIKDSDHLVAKIRCSSKGYCFAVRGKDKEDIYIKENLLNYAWNGDKVL
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCEEEECCCCCCCEEEEEHHHHHCCCCCHHH
VRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVYAIPIDDRILSKIKLPKENKK
HHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHCCCEEEEECCHHHHHHCCCCCCCCC
YIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELTLNNNEEYDSDFVLSKSNIAKSYNLNH
EEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
IESKKIEKRERIDLTDKNSYLFKSWSSNNSPMLPMIQIEQVKNNNTKLWIHTNNLAERVD
CCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEEEECCHHHHCC
LNSKKSQEILLKRFESLPLLNDWENYLSEAIRNDSEFKLGKKSEAISLCVHLNSDNEIID
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEE
WSFHLTLVKCSLIVGSDHTDALLSRKSKSRITSRVLKPIKEYIEDLDKILEISFSFRQKH
EEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEGKVEIPAPVNKIKAIEEFFIHNPAEYSKGYFESLNKEDCQTFLSPILYEANLIWFKH
HHCCCEECCCCHHHHHHHHHHHCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHCEEEEEEE
SNKYGLKSAGYISKGIDYVNANEIIKYSEFIDNDMELNEDGNLTFSQVIKICGDDNKKRI
CCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHCCCCHHHH
LHKLLINEFEDNEIRLISKNPDNDESEKLFISPWTIPSFDFTNLINQYCIFNMIINGKKS
HHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCC
KKNNINEINISESNSLDLVNWDIFNSSISKNLEALFNKLLIDKHNEFKYKVNQYKSNMIS
CCCCCCEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
IKKVRKAEKLLGNTYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLI
HHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEEECCCHHHHHHCCCCEEE
GRKSKKSYKVGDAIEVKIIKVDILKYQIDLELT
ECCCCCCCCCCCEEEEEEEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7727754; 8905231 [H]