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The map label for this gene is pheT [H]

Identifier: 126696327

GI number: 126696327

Start: 860684

End: 863128

Strand: Reverse

Name: pheT [H]

Synonym: P9301_09891

Alternate gene names: 126696327

Gene position: 863128-860684 (Counterclockwise)

Preceding gene: 126696333

Following gene: 126696326

Centisome position: 52.57

GC content: 30.02

Gene sequence:

>2445_bases
ATGAAAGTTTCTCAAAATTGGTTGAAAAATTTAGTAGAAATTACTTCTACTCCTGAAGATCTCTCTGAGAAATTGTCTAT
CGGTGGATTTGAGGTTGAATCATTAGAAGATTGTTCAAAAAATGTAAATGGTGTTGTTTTAGGTAAGGTATTATCAGTTT
TAAAACACGAAGGATCTGACAAACTTTCAATTTGCCAAGTCGATATTGGTAATTCAAAGAATTTACAAATTATCTGTGGT
GCGCGCAATATTAAACCAAATATTTATGTTTATGTTGCTACTGTCGGCACGAAATTAAATGCAGTTGATTTAACTATTAG
AAGAAGTGAAATTAGAGGTGTCATGAGTGAAGGAATGATATGTTCACTGCAGGAACTGGGCTTAAAGGACTCTAGTGAAG
GGATAGAGATCATTGATGAGGATTTGGCCCTAAAACATGAATTGGGTACTCCAGGGTCTGACATTCTTCAATTAAATGAT
TTTATATATGATTTAGCCATTACAGCTAATAGACCTGATGGAATGTCAGTTATAGGTATAGCCCGTGAAATTTCTGCTCT
TTTAGAATCCACCTTAAATTTTCCAGAATTAAACCATAAATACAATATTCATTTACTTAAAGGAATTAAACTTTGTCCAG
AGGCTATAGAATTTAATTGCATTTATACAATAAGCTGCATTGATGGAGTAAATGGTGAGAAATTATCACCAAATTGGCTT
AAAGACCGTATAGAAAAATCAGGTATAAAATCTATTAATCTCCTAGTTGATTTGACAAATTATATTCTTTTAGAACAAGG
TCAGCCTTTGCATGCGTTTGATAAAGATAAACTGTCAAACTTAATTGGTAAGGAAGTTTCTCCAGAAGATTTCTCTGTAA
GAAAAGGCAAGGATAACGAAAGCCTTTTTTGCTTAGATGGTAAAGAATATAAATTAAATGACAATATCACAGTTATTACT
TGTTGTGATAAACCGGTAGCTATTGCAGGGGTAATAGGCGGTTTAGAGACTTCTGTAAGTAATACTACCTCATCTATTTA
CCTTGAAGGCGCTGTTTTTAATCCAGTTACTATAAGAAAATCTTCCAAGGCGGTTGGCATAAGAACAGAATCTAGCAGCA
GGTATGAAAAAGGGATTTCATCAAAAAATACAATAAATGCAGTAACAAGGGCAATTAATCTTTTAGAAGAATATTTTTCT
ATTAATTCACCAATCATCAATACTTCAAATTTAATAAGTAATGAGGATATATTTATTAAACTACGGAGGAATCGAATACA
TAAAATTCTTGGCCCATTACTAATTAACGATCAATTTGAAAAAAGAAATTTATCTGATAATGAAATAGTTGATAAATTAA
CACTCATAGGTTGTACTTTAAAGAATAAAGAATATGGCTGGGATGTAGCAGTAATTCCTAATAGATCACATGATTTAATA
AGAGAAATAGATTTAATTGAAGAAATAGCAAGATTAATAGGGTATGACAGATTCGACTTAAAACTTCCTAATCCAATTAA
GCCTGGTAAATTGTCATCAGAACAGTTGGCATTGAGAAAAGTAAAAAATGGTTTTATAGAAAATGGTTTTAACGAGGTAC
TAAGCTATTCTCTTGTTCCTGAAGATGATGAAAAACTTATAAAGATTTCTAATCCTTTGTTATTAGAAACAAGCTGTCTT
AGAGATAATATCTGGAAAGAACATTTGGAGATAGTGAACCGTAATATAAAAGCTGGTCAAGTAAGTTGTTATATATTTGA
AATTGGAAATGTTTTTCAAAATAAATCTGAGTTTATTCAAAAAGAAGTTCTAAATGGTGCGATTTATGGAAATAAAAAAT
TTGGAAAATGGATAAATTCAGGTAAGGATAATGATCTAAATTATTTCCAGGCCAGAGGAAAGTTAAAGGAGGCTTTATCA
TCTTTGAACATAAAAATTGATGATAAACCTACTGATTCAATTGATTTTCTTCATCCAGGAAGAACAGCGAAATTAGTTAT
TGAAGGGAAAGATGCAGGTTATTTTGGTGAAATACATCCCAAACTAATATTAGAGAAGAAGTCATTAAAAAAAGTTTATT
TATTTAACATAAATGTCTCTAACCTCTTGGGAGCTAGTACAAGAAAAAATAAATGGATTCCAATATATAAGCAATACCCA
ATTGTTCCGAAAATCGAAAGAGATATAAATTTTGTTTTCAGTAAGAAATTTCTAATTAGCGAAATAACATCACAAATAAA
AAAAACAGGAAAAAATCTCTTAGAGGATGTAAATTTACTTGATGTTTTTGAAGATACTAAATTTGGAGATGATCATATAA
GTTATACATTTAGATTATCTTATAGAGATAAAGATAAAACATTACTAGACTCGGACATCACATCTATACATTCAAATATC
ATTTCTAATATTGAAAAAAGTTTTAACACTAAATTGAGGAATTAA

Upstream 100 bases:

>100_bases
TTTAATTAATACTTTATTGTATAATTTTCTATTGTACATCTTCAACAAACTTTTTTGAAGATATGCTCATTTCATTTATT
ATCATTGATTAAAAATATAT

Downstream 100 bases:

>100_bases
TTGTATTGCTAATCTCATATAAGTCTATAAACTAGTCTAATTATGATTCTTGTATAAGATAAATAATAATCCTATAAAAG
TAATTAATGTTGTATGATAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MKVSQNWLKNLVEITSTPEDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHEGSDKLSICQVDIGNSKNLQIICG
ARNIKPNIYVYVATVGTKLNAVDLTIRRSEIRGVMSEGMICSLQELGLKDSSEGIEIIDEDLALKHELGTPGSDILQLND
FIYDLAITANRPDGMSVIGIAREISALLESTLNFPELNHKYNIHLLKGIKLCPEAIEFNCIYTISCIDGVNGEKLSPNWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNESLFCLDGKEYKLNDNITVIT
CCDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTINAVTRAINLLEEYFS
INSPIINTSNLISNEDIFIKLRRNRIHKILGPLLINDQFEKRNLSDNEIVDKLTLIGCTLKNKEYGWDVAVIPNRSHDLI
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIENGFNEVLSYSLVPEDDEKLIKISNPLLLETSCL
RDNIWKEHLEIVNRNIKAGQVSCYIFEIGNVFQNKSEFIQKEVLNGAIYGNKKFGKWINSGKDNDLNYFQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLVIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLGASTRKNKWIPIYKQYP
IVPKIERDINFVFSKKFLISEITSQIKKTGKNLLEDVNLLDVFEDTKFGDDHISYTFRLSYRDKDKTLLDSDITSIHSNI
ISNIEKSFNTKLRN

Sequences:

>Translated_814_residues
MKVSQNWLKNLVEITSTPEDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHEGSDKLSICQVDIGNSKNLQIICG
ARNIKPNIYVYVATVGTKLNAVDLTIRRSEIRGVMSEGMICSLQELGLKDSSEGIEIIDEDLALKHELGTPGSDILQLND
FIYDLAITANRPDGMSVIGIAREISALLESTLNFPELNHKYNIHLLKGIKLCPEAIEFNCIYTISCIDGVNGEKLSPNWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNESLFCLDGKEYKLNDNITVIT
CCDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTINAVTRAINLLEEYFS
INSPIINTSNLISNEDIFIKLRRNRIHKILGPLLINDQFEKRNLSDNEIVDKLTLIGCTLKNKEYGWDVAVIPNRSHDLI
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIENGFNEVLSYSLVPEDDEKLIKISNPLLLETSCL
RDNIWKEHLEIVNRNIKAGQVSCYIFEIGNVFQNKSEFIQKEVLNGAIYGNKKFGKWINSGKDNDLNYFQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLVIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLGASTRKNKWIPIYKQYP
IVPKIERDINFVFSKKFLISEITSQIKKTGKNLLEDVNLLDVFEDTKFGDDHISYTFRLSYRDKDKTLLDSDITSIHSNI
ISNIEKSFNTKLRN
>Mature_814_residues
MKVSQNWLKNLVEITSTPEDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHEGSDKLSICQVDIGNSKNLQIICG
ARNIKPNIYVYVATVGTKLNAVDLTIRRSEIRGVMSEGMICSLQELGLKDSSEGIEIIDEDLALKHELGTPGSDILQLND
FIYDLAITANRPDGMSVIGIAREISALLESTLNFPELNHKYNIHLLKGIKLCPEAIEFNCIYTISCIDGVNGEKLSPNWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNESLFCLDGKEYKLNDNITVIT
CCDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTINAVTRAINLLEEYFS
INSPIINTSNLISNEDIFIKLRRNRIHKILGPLLINDQFEKRNLSDNEIVDKLTLIGCTLKNKEYGWDVAVIPNRSHDLI
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIENGFNEVLSYSLVPEDDEKLIKISNPLLLETSCL
RDNIWKEHLEIVNRNIKAGQVSCYIFEIGNVFQNKSEFIQKEVLNGAIYGNKKFGKWINSGKDNDLNYFQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLVIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLGASTRKNKWIPIYKQYP
IVPKIERDINFVFSKKFLISEITSQIKKTGKNLLEDVNLLDVFEDTKFGDDHISYTFRLSYRDKDKTLLDSDITSIHSNI
ISNIEKSFNTKLRN

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=834, Percent_Identity=29.3764988009592, Blast_Score=352, Evalue=8e-98,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 91487; Mature: 91487

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSQNWLKNLVEITSTPEDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHEGSD
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHCCCCC
KLSICQVDIGNSKNLQIICGARNIKPNIYVYVATVGTKLNAVDLTIRRSEIRGVMSEGMI
CEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEEHHHHHHHHHCCCE
CSLQELGLKDSSEGIEIIDEDLALKHELGTPGSDILQLNDFIYDLAITANRPDGMSVIGI
EEHHHCCCCCCCCCCEEECHHHHHHHHCCCCCHHHHHHHHEEEEEEEECCCCCCCEEHHH
AREISALLESTLNFPELNHKYNIHLLKGIKLCPEAIEFNCIYTISCIDGVNGEKLSPNWL
HHHHHHHHHHHCCCCCCCCEEEEEEECCCHHCHHHHCEEEEEEEEEECCCCCCCCCCHHH
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNE
HHHHHHCCCCEEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCC
SLFCLDGKEYKLNDNITVITCCDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRK
CEEEECCCEEEECCCEEEEEECCCCEEEHHHHCCCHHCCCCCCCEEEEECEEECCEEEEC
SSKAVGIRTESSSRYEKGISSKNTINAVTRAINLLEEYFSINSPIINTSNLISNEDIFIK
CCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
LRRNRIHKILGPLLINDQFEKRNLSDNEIVDKLTLIGCTLKNKEYGWDVAVIPNRSHDLI
EHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHEEEEEECCCCCCCEEEEECCCCHHHH
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIENGFNEVLSYSLVP
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHEECCCC
EDDEKLIKISNPLLLETSCLRDNIWKEHLEIVNRNIKAGQVSCYIFEIGNVFQNKSEFIQ
CCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHCCHHHHHH
KEVLNGAIYGNKKFGKWINSGKDNDLNYFQARGKLKEALSSLNIKIDDKPTDSIDFLHPG
HHHHCCEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCC
RTAKLVIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLGASTRKNKWIPIYKQYP
CEEEEEEECCCCCCCCCCCHHEEEECCCCCEEEEEEEEHHHHHCCCCCCCCCEEEHCCCC
IVPKIERDINFVFSKKFLISEITSQIKKTGKNLLEDVNLLDVFEDTKFGDDHISYTFRLS
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEEE
YRDKDKTLLDSDITSIHSNIISNIEKSFNTKLRN
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVSQNWLKNLVEITSTPEDLSEKLSIGGFEVESLEDCSKNVNGVVLGKVLSVLKHEGSD
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHCCCCC
KLSICQVDIGNSKNLQIICGARNIKPNIYVYVATVGTKLNAVDLTIRRSEIRGVMSEGMI
CEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEEHHHHHHHHHCCCE
CSLQELGLKDSSEGIEIIDEDLALKHELGTPGSDILQLNDFIYDLAITANRPDGMSVIGI
EEHHHCCCCCCCCCCEEECHHHHHHHHCCCCCHHHHHHHHEEEEEEEECCCCCCCEEHHH
AREISALLESTLNFPELNHKYNIHLLKGIKLCPEAIEFNCIYTISCIDGVNGEKLSPNWL
HHHHHHHHHHHCCCCCCCCEEEEEEECCCHHCHHHHCEEEEEEEEEECCCCCCCCCCHHH
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNE
HHHHHHCCCCEEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCC
SLFCLDGKEYKLNDNITVITCCDKPVAIAGVIGGLETSVSNTTSSIYLEGAVFNPVTIRK
CEEEECCCEEEECCCEEEEEECCCCEEEHHHHCCCHHCCCCCCCEEEEECEEECCEEEEC
SSKAVGIRTESSSRYEKGISSKNTINAVTRAINLLEEYFSINSPIINTSNLISNEDIFIK
CCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
LRRNRIHKILGPLLINDQFEKRNLSDNEIVDKLTLIGCTLKNKEYGWDVAVIPNRSHDLI
EHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHEEEEEECCCCCCCEEEEECCCCHHHH
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIENGFNEVLSYSLVP
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHEECCCC
EDDEKLIKISNPLLLETSCLRDNIWKEHLEIVNRNIKAGQVSCYIFEIGNVFQNKSEFIQ
CCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHCCHHHHHH
KEVLNGAIYGNKKFGKWINSGKDNDLNYFQARGKLKEALSSLNIKIDDKPTDSIDFLHPG
HHHHCCEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCC
RTAKLVIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLGASTRKNKWIPIYKQYP
CEEEEEEECCCCCCCCCCCHHEEEECCCCCEEEEEEEEHHHHHCCCCCCCCCEEEHCCCC
IVPKIERDINFVFSKKFLISEITSQIKKTGKNLLEDVNLLDVFEDTKFGDDHISYTFRLS
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEEE
YRDKDKTLLDSDITSIHSNIISNIEKSFNTKLRN
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA