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The map label for this gene is prfC [H]

Identifier: 126696054

GI number: 126696054

Start: 636887

End: 638524

Strand: Direct

Name: prfC [H]

Synonym: P9301_07161

Alternate gene names: 126696054

Gene position: 636887-638524 (Clockwise)

Preceding gene: 126696053

Following gene: 126696055

Centisome position: 38.79

GC content: 35.29

Gene sequence:

>1638_bases
ATGAGCTTAGGTACTAAAATTCTAAATAATAAAGACATACTGGAAGCGGTAAATAAAAGAAGAAATTTTGCTATTATTTC
ACATCCGGATGCTGGGAAAACGACTCTTACTGAGAAGCTTCTTTTGTATGGAGGTGCCATTCAACAAGCAGGAGCCGTAA
AAGCTAGGGGTAATCAGAGAAAAGCAACCTCAGACTGGATGGAACTTGAGAAACAAAGAGGTATATCTATAACATCAACT
GTATTGCAATTTGAATATGAAAGATCAGTAATCAATCTATTAGATACACCAGGACACCAAGATTTCTCCGAAGATACTTA
TAGAACATTAGCTGCTGCTGATAATGCAGTTATGTTGGAAGATGCTGCTAAAGGACTAGAACCTCAAACTAGAAAATTGT
TTGAAGTTTGCAAGATGCGAAAAATACCAATATTTACTTTCATAAATAAAATGGATAGACCAGGAAGAGAACCATTTTCT
TTACTTGATGAAATTGAATCAGAACTTGGATTAAATACCCTACCTATAAACTGGCCAATTGGGAGTGGCGAAGAATTTAG
AGGGGTTATTGATAGATTTTCGAGAGAGGTGATTTTATTTGATAAAGCAGTGAGAGGGAAACAATCGAATGAGAAAAGAT
TAAGTCTTGAAGATAAAGAGGTATCAAAATATGTAGAGAGAGATTTACTTGAAAACTCACTTGAAGAATTGGAGGTTCTT
GATGAGGCAGGATCTAAATTTGAAAAAGAAAAAGTTTTCAATGGCTCTTTAACCCCAGTTTTCTTTGGATCTGCCATGAC
TAATTTTGGCGTAAGGCCATTTTTAGATAGTTTTTTAAAAATGGCACAAAAACCAACGTCAAGAAATAGTAATAAAGGGG
ATATTGAACCTGCAAGCGATGAATTTAGTGGGTTTGTTTTTAAGCTTCAGGCAAATATGGATCCAAAGCACAGAGATAGG
GTTGCTTTTGTAAGAGTTTGTAGTGGTAAATTTGAAAAGGATATGTCAGTTAAACATTCCAGAACTGGGAAAACAATTAG
ATTATCAAGACCACAAAAAATATTTGGGCAAGATAGAGAAGTAGTTGATGATGCCTATCCTGGAGATGTTATTGGTTTAA
ATAATCCAGGGATGTTTTCTATTGGAGATACTCTTTATACTGGTACTCATCTGGAATATGAGGGCATACCATCCTTTAGT
CCTGAAATATTCAGCTGGCTAAGAAATCCAAATCCCTCAGCATTTAAAAATTTTAGAAAGGGTGTTAATGAACTTCGAGA
AGAAGGTGCAGTTCAGATTCTTTATGACTTTGATGAGAGCAAAAGAGACCCCATACTCGCAGCCGTTGGTCAATTGCAGT
TGGAGGTAGTAACTCACAGATTAAAAAGTGAATATGGTGTAGATGCAAATCTTGAAGCAATGCCATATCAATTGGCTAGA
TGGATTTCTGATGGATGGCCAGCCATTGAAAAACTAGGCAGAATATTCAACTGTAAAATAGTTAAAGATTGTTGGAATAG
GCCAGTTATTCTTTTCAAAAATGAGTGGAATCTAAATCAATTTGTTGAAGACAATAATCAATTAAATTTAAACAAAGTTG
CGCCCGTTGTTAGTGGAGTCGAACCAATTGTTTTATAA

Upstream 100 bases:

>100_bases
ATGATCTATCCTCTTTAGATGTTTTTGAAGACGGTGATAATTATTTAATAGTCTATGGTAATGTTTTAAAAACTAATTGA
AAGGCCCTTATAGTAAATAA

Downstream 100 bases:

>100_bases
AGTCTTAATGGATTATAAAATTAGTTAATCTGTTAGTATTGGTAAAAAATTTTGGATTTAAACAGTAATAAAAATAATAA
CGAAAAATCACCTTATGAAA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSLGTKILNNKDILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMELEKQRGISITST
VLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRPGREPFS
LLDEIESELGLNTLPINWPIGSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKEVSKYVERDLLENSLEELEVL
DEAGSKFEKEKVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASDEFSGFVFKLQANMDPKHRDR
VAFVRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTHLEYEGIPSFS
PEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKSEYGVDANLEAMPYQLAR
WISDGWPAIEKLGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNQLNLNKVAPVVSGVEPIVL

Sequences:

>Translated_545_residues
MSLGTKILNNKDILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMELEKQRGISITST
VLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRPGREPFS
LLDEIESELGLNTLPINWPIGSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKEVSKYVERDLLENSLEELEVL
DEAGSKFEKEKVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASDEFSGFVFKLQANMDPKHRDR
VAFVRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTHLEYEGIPSFS
PEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKSEYGVDANLEAMPYQLAR
WISDGWPAIEKLGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNQLNLNKVAPVVSGVEPIVL
>Mature_544_residues
SLGTKILNNKDILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMELEKQRGISITSTV
LQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRPGREPFSL
LDEIESELGLNTLPINWPIGSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKEVSKYVERDLLENSLEELEVLD
EAGSKFEKEKVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASDEFSGFVFKLQANMDPKHRDRV
AFVRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTHLEYEGIPSFSP
EIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKSEYGVDANLEAMPYQLARW
ISDGWPAIEKLGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNQLNLNKVAPVVSGVEPIVL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=493, Percent_Identity=31.237322515213, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI19923640, Length=530, Percent_Identity=26.2264150943396, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=29.0076335877863, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI25306287, Length=502, Percent_Identity=26.4940239043825, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI157426893, Length=182, Percent_Identity=29.6703296703297, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=155, Percent_Identity=32.9032258064516, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=32.8859060402685, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310132016, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310110807, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310123363, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=471, Percent_Identity=50.5307855626327, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789738, Length=499, Percent_Identity=28.8577154308617, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=32.2404371584699, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=36.9127516778523, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=28.3057851239669, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17556745, Length=478, Percent_Identity=27.1966527196653, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=33.5526315789474, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.055900621118, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=484, Percent_Identity=30.1652892561983, Blast_Score=204, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6322359, Length=513, Percent_Identity=26.9005847953216, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=32.0754716981132, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=32.0754716981132, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582462, Length=491, Percent_Identity=30.9572301425662, Blast_Score=205, Evalue=8e-53,
Organism=Drosophila melanogaster, GI221458488, Length=523, Percent_Identity=24.8565965583174, Blast_Score=142, Evalue=9e-34,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=32.183908045977, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=31.6901408450704, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=200, Percent_Identity=30, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17864358, Length=200, Percent_Identity=30, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21357743, Length=147, Percent_Identity=29.9319727891156, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 61734; Mature: 61603

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGTKILNNKDILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQR
CCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCC
KATSDWMELEKQRGISITSTVLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEH
DAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRPGREPFSLLDEIESELGLNTLPINWPI
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCC
GSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKEVSKYVERDLLENSLEELEVL
CCCHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEAGSKFEKEKVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASD
HHHCCHHHHHHHCCCCCCCEEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFSGFVFKLQANMDPKHRDRVAFVRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDRE
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEECCCHHHHCCCHH
VVDDAYPGDVIGLNNPGMFSIGDTLYTGTHLEYEGIPSFSPEIFSWLRNPNPSAFKNFRK
HHHCCCCCCEEECCCCCCEEECCCEECCCCEEECCCCCCCHHHHHHHCCCCHHHHHHHHH
GVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKSEYGVDANLEAMPYQLAR
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
WISDGWPAIEKLGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNQLNLNKVAPVVSGV
HHCCCCHHHHHHHHHHCCEEEHHHHCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCC
EPIVL
CCCCC
>Mature Secondary Structure 
SLGTKILNNKDILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQR
CCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCC
KATSDWMELEKQRGISITSTVLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEH
DAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRPGREPFSLLDEIESELGLNTLPINWPI
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCC
GSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKEVSKYVERDLLENSLEELEVL
CCCHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEAGSKFEKEKVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASD
HHHCCHHHHHHHCCCCCCCEEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFSGFVFKLQANMDPKHRDRVAFVRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDRE
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEECCCHHHHCCCHH
VVDDAYPGDVIGLNNPGMFSIGDTLYTGTHLEYEGIPSFSPEIFSWLRNPNPSAFKNFRK
HHHCCCCCCEEECCCCCCEEECCCEECCCCEEECCCCCCCHHHHHHHCCCCHHHHHHHHH
GVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKSEYGVDANLEAMPYQLAR
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
WISDGWPAIEKLGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNQLNLNKVAPVVSGV
HHCCCCHHHHHHHHHHCCEEEHHHHCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCC
EPIVL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA