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The map label for this gene is murF [H]

Identifier: 126695974

GI number: 126695974

Start: 564394

End: 565767

Strand: Direct

Name: murF [H]

Synonym: P9301_06361

Alternate gene names: 126695974

Gene position: 564394-565767 (Clockwise)

Preceding gene: 126695973

Following gene: 126695978

Centisome position: 34.37

GC content: 28.09

Gene sequence:

>1374_bases
ATGGATTTCTCTTTTTTTGAAATAAAAGATGTTTTGGGTGATATTAAAAATCTGGGCGAGGGGGGAATAGATTCTTTAAA
TTTCAAGAATATATGTATTGATAGTAGAACTTGTTTGAAAAATGATCTTTTTATAGCTATCAAGGGTAAAAATTTTGACG
GACATAATTTTCTTCCAGACGTTTTAAATAAAGGGGTTAAATCAGTAGTTATAAAAGAAGGTATGCAGAAATTACTTCCT
GATAATTTTCCTTGTTGGGTTGTTAGTGACACTTTAAATGCATTTCAAAAATTAACCTTGCTCAAAAGAAAAAAATTAAG
TATCCCTGTGGTTGCCATAACTGGTTCAGTCGGTAAAACAACTACAAAAGAAATGGTTGGTGAGGTTTTAAATAAACTTG
GAAAAATTAAATTATCCCATGCAAATTTTAATAATGAGATTGGGGTTGGTCTCACTATTCTTGCTACAGATAAAGAAGAT
AAAGTTTTGGTCCTTGAGATGGGAATGAGAGGTCTTGGACAGATCGAAAATTTATCTAAATATAGTAAACCCGATATTGC
AGTAATTACTAACATTGGGACAGCACATATTGGATTGTTAGGCTCTAAAAAAAATATTACTTATGCTAAGTGTGAAATTA
GTAAATTTTTAAATCCCAAAGGAGTTGTAATAATACCAGCTAATGATTTACTTCTTGAAGAAACTTTAAGAGAATACTGG
AAAGGTAGGGTAACAAAAGTAGAACTTCTTAATATAGAAAATCAAAATGACAGTTTTAAGAAAGATAATAATTTGCGAGG
ATTTTATAATCCCTCTAATAAAACAATCTTAATCGAAGAAAATATCTTTGAAATTTCATTTGAGGGATTTCACAATGCTT
CAAATTTCCTATTGGCTTATGCAGTTGCCAAAGAGCTAGGCATTGATTTTGAAAGTTTTAATAAATTTGATTTTGTAAGT
TTAGGTGGAAGAAATAAAATTCTTAAATCAGTAAAAACAACAATATATGATGAATCATATAATGCTTCGCCAGAGTCAGT
AAAAGCATGTATTAAAACCCTCCTTGAAAAACCGAGAAATAAATTTTTCATATTTGGAAGTATGCAAGAATTGGGAGAAG
AATCTGAAAAATTTCATAAAGAAATATTCAACTTAATAAATAATTCAGACATAGAAAAGTGTTTATTTATTTGTGATAAA
AAAAATAAAAAAATTTACACCAATTATCTCAAAGATAAGAAAAAATTTTTAGTTTTAAATAATATTAAAGATGTACCTAA
AGAGATAAACAAATCCACAAAAAAAGGTGATTCTATTCTTATTAAAGGGAGCAGAAGTTGGCAACTTGAAAAAATTATTG
AATTAATCAATTAG

Upstream 100 bases:

>100_bases
CGGAATAAAGGAACCATCACCAGATGTTGCTGAAGTTGAGAAATTTTCTTATGGACAATCAGCTGATCCATCTTTAAAAA
AAGTATGACTTAAATTCTTA

Downstream 100 bases:

>100_bases
ATCAGGATTTCTTTCTTTCCCAATTTTCTATATTTACTTGCTGAGTTCTTGAGATTGCTAATGAATTATCTTTTGAGTCT
TTTGTGATCACTGAACCAGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDFSFFEIKDVLGDIKNLGEGGIDSLNFKNICIDSRTCLKNDLFIAIKGKNFDGHNFLPDVLNKGVKSVVIKEGMQKLLP
DNFPCWVVSDTLNAFQKLTLLKRKKLSIPVVAITGSVGKTTTKEMVGEVLNKLGKIKLSHANFNNEIGVGLTILATDKED
KVLVLEMGMRGLGQIENLSKYSKPDIAVITNIGTAHIGLLGSKKNITYAKCEISKFLNPKGVVIIPANDLLLEETLREYW
KGRVTKVELLNIENQNDSFKKDNNLRGFYNPSNKTILIEENIFEISFEGFHNASNFLLAYAVAKELGIDFESFNKFDFVS
LGGRNKILKSVKTTIYDESYNASPESVKACIKTLLEKPRNKFFIFGSMQELGEESEKFHKEIFNLINNSDIEKCLFICDK
KNKKIYTNYLKDKKKFLVLNNIKDVPKEINKSTKKGDSILIKGSRSWQLEKIIELIN

Sequences:

>Translated_457_residues
MDFSFFEIKDVLGDIKNLGEGGIDSLNFKNICIDSRTCLKNDLFIAIKGKNFDGHNFLPDVLNKGVKSVVIKEGMQKLLP
DNFPCWVVSDTLNAFQKLTLLKRKKLSIPVVAITGSVGKTTTKEMVGEVLNKLGKIKLSHANFNNEIGVGLTILATDKED
KVLVLEMGMRGLGQIENLSKYSKPDIAVITNIGTAHIGLLGSKKNITYAKCEISKFLNPKGVVIIPANDLLLEETLREYW
KGRVTKVELLNIENQNDSFKKDNNLRGFYNPSNKTILIEENIFEISFEGFHNASNFLLAYAVAKELGIDFESFNKFDFVS
LGGRNKILKSVKTTIYDESYNASPESVKACIKTLLEKPRNKFFIFGSMQELGEESEKFHKEIFNLINNSDIEKCLFICDK
KNKKIYTNYLKDKKKFLVLNNIKDVPKEINKSTKKGDSILIKGSRSWQLEKIIELIN
>Mature_457_residues
MDFSFFEIKDVLGDIKNLGEGGIDSLNFKNICIDSRTCLKNDLFIAIKGKNFDGHNFLPDVLNKGVKSVVIKEGMQKLLP
DNFPCWVVSDTLNAFQKLTLLKRKKLSIPVVAITGSVGKTTTKEMVGEVLNKLGKIKLSHANFNNEIGVGLTILATDKED
KVLVLEMGMRGLGQIENLSKYSKPDIAVITNIGTAHIGLLGSKKNITYAKCEISKFLNPKGVVIIPANDLLLEETLREYW
KGRVTKVELLNIENQNDSFKKDNNLRGFYNPSNKTILIEENIFEISFEGFHNASNFLLAYAVAKELGIDFESFNKFDFVS
LGGRNKILKSVKTTIYDESYNASPESVKACIKTLLEKPRNKFFIFGSMQELGEESEKFHKEIFNLINNSDIEKCLFICDK
KNKKIYTNYLKDKKKFLVLNNIKDVPKEINKSTKKGDSILIKGSRSWQLEKIIELIN

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=461, Percent_Identity=26.8980477223427, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51593; Mature: 51593

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSFFEIKDVLGDIKNLGEGGIDSLNFKNICIDSRTCLKNDLFIAIKGKNFDGHNFLPD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHCCCCEEEEEECCCCCCCCCCHH
VLNKGVKSVVIKEGMQKLLPDNFPCWVVSDTLNAFQKLTLLKRKKLSIPVVAITGSVGKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
TTKEMVGEVLNKLGKIKLSHANFNNEIGVGLTILATDKEDKVLVLEMGMRGLGQIENLSK
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHH
YSKPDIAVITNIGTAHIGLLGSKKNITYAKCEISKFLNPKGVVIIPANDLLLEETLREYW
CCCCCEEEEEECCCEEEEEEECCCCCEEEEEEHHHCCCCCCEEEECCCHHHHHHHHHHHH
KGRVTKVELLNIENQNDSFKKDNNLRGFYNPSNKTILIEENIFEISFEGFHNASNFLLAY
CCCCEEEEEEEECCCCCCCCCCCCCCCEECCCCCEEEEECCEEEEEECCCCCCHHHHHHH
AVAKELGIDFESFNKFDFVSLGGRNKILKSVKTTIYDESYNASPESVKACIKTLLEKPRN
HHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
KFFIFGSMQELGEESEKFHKEIFNLINNSDIEKCLFICDKKNKKIYTNYLKDKKKFLVLN
CEEEEECHHHHCHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCEEHHHHHHCCCEEEEEC
NIKDVPKEINKSTKKGDSILIKGSRSWQLEKIIELIN
CHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHC
>Mature Secondary Structure
MDFSFFEIKDVLGDIKNLGEGGIDSLNFKNICIDSRTCLKNDLFIAIKGKNFDGHNFLPD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHCCCCEEEEEECCCCCCCCCCHH
VLNKGVKSVVIKEGMQKLLPDNFPCWVVSDTLNAFQKLTLLKRKKLSIPVVAITGSVGKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
TTKEMVGEVLNKLGKIKLSHANFNNEIGVGLTILATDKEDKVLVLEMGMRGLGQIENLSK
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHH
YSKPDIAVITNIGTAHIGLLGSKKNITYAKCEISKFLNPKGVVIIPANDLLLEETLREYW
CCCCCEEEEEECCCEEEEEEECCCCCEEEEEEHHHCCCCCCEEEECCCHHHHHHHHHHHH
KGRVTKVELLNIENQNDSFKKDNNLRGFYNPSNKTILIEENIFEISFEGFHNASNFLLAY
CCCCEEEEEEEECCCCCCCCCCCCCCCEECCCCCEEEEECCEEEEEECCCCCCHHHHHHH
AVAKELGIDFESFNKFDFVSLGGRNKILKSVKTTIYDESYNASPESVKACIKTLLEKPRN
HHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
KFFIFGSMQELGEESEKFHKEIFNLINNSDIEKCLFICDKKNKKIYTNYLKDKKKFLVLN
CEEEEECHHHHCHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCEEHHHHHHCCCEEEEEC
NIKDVPKEINKSTKKGDSILIKGSRSWQLEKIIELIN
CHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]