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The map label for this gene is proA

Identifier: 126695954

GI number: 126695954

Start: 542625

End: 543935

Strand: Direct

Name: proA

Synonym: P9301_06161

Alternate gene names: 126695954

Gene position: 542625-543935 (Clockwise)

Preceding gene: 126695953

Following gene: 126695955

Centisome position: 33.05

GC content: 32.49

Gene sequence:

>1311_bases
ATGGCTAATATCTTTGAAGTGCCTAATCCAGGTAATGATCTTCTAGAAAAAGCTGATAAAGTTCGTTTGGCATCAATAAA
AATAAGTCAGACTGAAAATATAGATCGAATTAAAGCCTTAAATTTTATGGCTGATTGTCTAGAAAAAAATTCTAAAGAAA
TATTAGAGGCCAATAGTGCCGATTATTCAAGTGCAGAAAAAAAAGGTATTTCTAGGGCTTTACTTTCTAGATTAAAGCTT
TCAAAAGCAAAATTAAATTCAGGAATTGAAGGAGTAAGAAAAGTTGGAGAATTGGCTGATCCTGTAAATCAAATTCAAAT
AAAAAGAGAGCTTTCAAATGGATTGATCTTAGAAAGAAAAACTGTGCCAATTGGAGTCTTAGGGGTTATTTTTGAATCAA
GGCCAGATGCCGTGATGCAGATTAGTTCTTTAGCAATAAGATCAGGTAACGGAGTAATTCTAAAGGGCGGAAGTGAAGCC
AATTTAACAAATACTTCAATAGTGAAAGCATTGCAAGAAGGTTTAGATGAATCGGGTCTAGATAAAAACGCAATATGTTT
ACTAACAAGCAGAAAAGATAGTATGGCGATGTTAAATCTTGAGAAATATATTAATTTAATAATTCCAAGAGGAAGCAATG
AATTGGTTAAATTTATTCAGGAGAATACAAGAATTCCTGTACTAGGCCATGCTGATGGAATTTGTCACTTGTTTATAGAT
ATTGAGGCCAATCTAGAGATGGCTTTATCAGTCGCTTTGGACAGCAAAATTCAATATCCTGCAGCATGTAATGCTATCGA
AACTTTATTAGTCCATAAAGATATCGCACCAGCTTTTTTAGAAAAAGCCATCCCTTTGTTTAATTCTAATGATGTTAAAT
TAATTGGAGATAGGAAATCAGTTGAATTAGGATTAGAACATGAGGCTAGTGCAGAAGATTGGCAAACTGAATATTTAGAT
TTAATTCTATCTATTAAAATTGTTGATGATCTTGATGAAGCAATCATACATATCCAAAAATATAGTTCAAAACATACAGA
TGGAATAATTACTGAAAATTTAAAGACTGCCAATAGATTTATGAATGTAGTTGATAGTGCGGGTGTTTTTCATAATTGTT
CTACAAGGTTTGCAGATGGATTTAGATATGGATTCGGTGCAGAAGTTGGGATATCAACTCAAACTCTTCCACCAAGGGGA
CCTGTAGGTCTAGAAGGTTTGGTAACTTATAAATATTTTCTAAAAGGTGATGGGAATATAGTTGATGATTTTTCATTAGG
AAAAGCTATCTATACACATAAAGATCTTTAA

Upstream 100 bases:

>100_bases
TGGAAAAGTCAAGTTCAATTATTTTTTAGATATTAACTCAGAAAAATTTACTTTAAAGAATCGTTAATTTGAATTATTAT
TAGATTAGCTAAAAAATATC

Downstream 100 bases:

>100_bases
AACTTTTAAAGATGGAAACTTTTTTAAAAATTGAGAATATTAAACTTTGGGCTAGGGTTGGCGTTCTTGATGAAGAAAGG
GAATTAGGGCAACTATTTAT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MANIFEVPNPGNDLLEKADKVRLASIKISQTENIDRIKALNFMADCLEKNSKEILEANSADYSSAEKKGISRALLSRLKL
SKAKLNSGIEGVRKVGELADPVNQIQIKRELSNGLILERKTVPIGVLGVIFESRPDAVMQISSLAIRSGNGVILKGGSEA
NLTNTSIVKALQEGLDESGLDKNAICLLTSRKDSMAMLNLEKYINLIIPRGSNELVKFIQENTRIPVLGHADGICHLFID
IEANLEMALSVALDSKIQYPAACNAIETLLVHKDIAPAFLEKAIPLFNSNDVKLIGDRKSVELGLEHEASAEDWQTEYLD
LILSIKIVDDLDEAIIHIQKYSSKHTDGIITENLKTANRFMNVVDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRG
PVGLEGLVTYKYFLKGDGNIVDDFSLGKAIYTHKDL

Sequences:

>Translated_436_residues
MANIFEVPNPGNDLLEKADKVRLASIKISQTENIDRIKALNFMADCLEKNSKEILEANSADYSSAEKKGISRALLSRLKL
SKAKLNSGIEGVRKVGELADPVNQIQIKRELSNGLILERKTVPIGVLGVIFESRPDAVMQISSLAIRSGNGVILKGGSEA
NLTNTSIVKALQEGLDESGLDKNAICLLTSRKDSMAMLNLEKYINLIIPRGSNELVKFIQENTRIPVLGHADGICHLFID
IEANLEMALSVALDSKIQYPAACNAIETLLVHKDIAPAFLEKAIPLFNSNDVKLIGDRKSVELGLEHEASAEDWQTEYLD
LILSIKIVDDLDEAIIHIQKYSSKHTDGIITENLKTANRFMNVVDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRG
PVGLEGLVTYKYFLKGDGNIVDDFSLGKAIYTHKDL
>Mature_435_residues
ANIFEVPNPGNDLLEKADKVRLASIKISQTENIDRIKALNFMADCLEKNSKEILEANSADYSSAEKKGISRALLSRLKLS
KAKLNSGIEGVRKVGELADPVNQIQIKRELSNGLILERKTVPIGVLGVIFESRPDAVMQISSLAIRSGNGVILKGGSEAN
LTNTSIVKALQEGLDESGLDKNAICLLTSRKDSMAMLNLEKYINLIIPRGSNELVKFIQENTRIPVLGHADGICHLFIDI
EANLEMALSVALDSKIQYPAACNAIETLLVHKDIAPAFLEKAIPLFNSNDVKLIGDRKSVELGLEHEASAEDWQTEYLDL
ILSIKIVDDLDEAIIHIQKYSSKHTDGIITENLKTANRFMNVVDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGP
VGLEGLVTYKYFLKGDGNIVDDFSLGKAIYTHKDL

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=436, Percent_Identity=43.348623853211, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI21361368, Length=436, Percent_Identity=43.348623853211, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1786438, Length=410, Percent_Identity=40.7317073170732, Blast_Score=311, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17569969, Length=430, Percent_Identity=43.7209302325581, Blast_Score=349, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17569967, Length=430, Percent_Identity=43.7209302325581, Blast_Score=349, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324899, Length=442, Percent_Identity=42.3076923076923, Blast_Score=342, Evalue=7e-95,
Organism=Drosophila melanogaster, GI21357643, Length=424, Percent_Identity=44.1037735849057, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_PROM0 (A3PBW4)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090840.1
- ProteinModelPortal:   A3PBW4
- SMR:   A3PBW4
- STRING:   A3PBW4
- GeneID:   4911322
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_06161
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   PMAR167546:P9301ORF_0631-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 47749; Mature: 47618

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIFEVPNPGNDLLEKADKVRLASIKISQTENIDRIKALNFMADCLEKNSKEILEANSA
CCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCC
DYSSAEKKGISRALLSRLKLSKAKLNSGIEGVRKVGELADPVNQIQIKRELSNGLILERK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEC
TVPIGVLGVIFESRPDAVMQISSLAIRSGNGVILKGGSEANLTNTSIVKALQEGLDESGL
CCCEEEEEEEECCCCHHHHHHHHHEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCC
DKNAICLLTSRKDSMAMLNLEKYINLIIPRGSNELVKFIQENTRIPVLGHADGICHLFID
CCCEEEEEECCCCCHHHEEHHHHEEEEECCCCHHHHHHHHHCCCCEEEECCCCEEEEEEE
IEANLEMALSVALDSKIQYPAACNAIETLLVHKDIAPAFLEKAIPLFNSNDVKLIGDRKS
EECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCC
VELGLEHEASAEDWQTEYLDLILSIKIVDDLDEAIIHIQKYSSKHTDGIITENLKTANRF
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MNVVDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGPVGLEGLVTYKYFLKGDGNI
HHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCE
VDDFSLGKAIYTHKDL
ECCCCCCCCHHCCCCC
>Mature Secondary Structure 
ANIFEVPNPGNDLLEKADKVRLASIKISQTENIDRIKALNFMADCLEKNSKEILEANSA
CCCCCCCCCCHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCC
DYSSAEKKGISRALLSRLKLSKAKLNSGIEGVRKVGELADPVNQIQIKRELSNGLILERK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEC
TVPIGVLGVIFESRPDAVMQISSLAIRSGNGVILKGGSEANLTNTSIVKALQEGLDESGL
CCCEEEEEEEECCCCHHHHHHHHHEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCC
DKNAICLLTSRKDSMAMLNLEKYINLIIPRGSNELVKFIQENTRIPVLGHADGICHLFID
CCCEEEEEECCCCCHHHEEHHHHEEEEECCCCHHHHHHHHHCCCCEEEECCCCEEEEEEE
IEANLEMALSVALDSKIQYPAACNAIETLLVHKDIAPAFLEKAIPLFNSNDVKLIGDRKS
EECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCC
VELGLEHEASAEDWQTEYLDLILSIKIVDDLDEAIIHIQKYSSKHTDGIITENLKTANRF
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MNVVDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGPVGLEGLVTYKYFLKGDGNI
HHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCE
VDDFSLGKAIYTHKDL
ECCCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA