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The map label for this gene is metK

Identifier: 126695674

GI number: 126695674

Start: 307344

End: 308585

Strand: Reverse

Name: metK

Synonym: P9301_03361

Alternate gene names: 126695674

Gene position: 308585-307344 (Counterclockwise)

Preceding gene: 126695675

Following gene: 126695673

Centisome position: 18.79

GC content: 36.15

Gene sequence:

>1242_bases
ATGAGTGATTTCATTTTCACTTCTGAATCCGTAACTGAAGGTCATCCTGACAAAATATGTGATCAAATTAGTGACGCAGT
TTTAGATGCTTTATTGACAGAAGATCCAGAAAGCAGAGTTGCATGCGAAACTGTCGTTAACACTGGTCTTTGTCTACTTA
CTGGAGAAATAACTTCAAAAGCAAAAGTCGATTACATAAAACTTGTTAGAAATGTAATTAAAGAAATTGGATATGAAGGC
TATAGAGCGGGTGGTTTTGATGCAAATAGTTGTGCTGTTTTAGTAGCACTTGACGAGCAATCACCAGATATTTCACAAGG
CGTAAACGACGCAGATGATATTAACGATGATTTGGAAGATAATACCGGAGCTGGTGACCAAGGCATAATGTTTGGCTATG
CGTGCGATGAGACGCCTGAATTAATGCCCTTACCTATTAGCCTAGCTCATAGATTAGCTATTCAACTTGCCAAAGTAAGG
CATGAAGAAGTCCTTAATTATCTACTCCCTGATGGGAAAACTCAAGTAAGCATTGATTATGAAAAAGGGTTACCAGTCTC
TATTAATACGATTTTAATTTCAACTCAACATAATTCTGAGATTGATGGCGTAACAAATGAAGAAGAAATTCGTCAAAGAA
TAAAAGAAGATTTATGGAAGAATGTTGTAATTCCTGCTACTGAAGATTTAGAAATTAAACCAAATATTAGTAAAACAAGA
TTTCTAGTAAACCCCACGGGAAAATTTGTCGTAGGCGGGCCTCAAGGAGATGCTGGGCTGACTGGCAGAAAAATTATTGT
AGATACTTATGGCGGATATGCAAGACACGGAGGAGGGGCATTTTCTGGTAAAGACCCCACAAAAGTAGATAGATCAGCCG
CTTATGCAGCACGTTATGTAGCTAAAAGTATAGTTAAGGCAAAATTAGCAAAAAAAGCAGAAGTACAATTAAGTTATGCA
ATTGGAGTAGCAAAACCAATTTCCATTCTCGTAGAAACTTTTGATACTGGTGTTATTTCACAAAATAATTTGACTGAGCT
AATTAAAGAGCACTTTGATTTAAGACCTGCAGCAATAATAAAAGAATTTAATTTAAGAAATCTACCAAAAAAAATGGGAG
GAAAATTTTTTAGAAAGACTGCATCCTATGGGCATTTTGGCAGAAGAGATCTTGAGCTACCTTGGGAAAATGTAGAAGAA
AAAGCAGCCAAATTAGCAGAGGCTTCCAAAATCTTTTTATAA

Upstream 100 bases:

>100_bases
CTAAAGTCTCATTATTTTATACAATTTTCTAATTTACAATAATTGATTTGTTACGATAGTTTAATTTAAGATGAAATCTA
ATTTTTAAAATTAATTGCAA

Downstream 100 bases:

>100_bases
ATATTTTTTTCTATGCCTGATAATTTTTATGGAGGGTTAGATTTTGGAACGAGTGGTGCAAGAATATCAATAATTAATCG
TCATAAGAAATTAGTATATT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEG
YRAGGFDANSCAVLVALDEQSPDISQGVNDADDINDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVR
HEEVLNYLLPDGKTQVSIDYEKGLPVSINTILISTQHNSEIDGVTNEEEIRQRIKEDLWKNVVIPATEDLEIKPNISKTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVETFDTGVISQNNLTELIKEHFDLRPAAIIKEFNLRNLPKKMGGKFFRKTASYGHFGRRDLELPWENVEE
KAAKLAEASKIFL

Sequences:

>Translated_413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEG
YRAGGFDANSCAVLVALDEQSPDISQGVNDADDINDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVR
HEEVLNYLLPDGKTQVSIDYEKGLPVSINTILISTQHNSEIDGVTNEEEIRQRIKEDLWKNVVIPATEDLEIKPNISKTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVETFDTGVISQNNLTELIKEHFDLRPAAIIKEFNLRNLPKKMGGKFFRKTASYGHFGRRDLELPWENVEE
KAAKLAEASKIFL
>Mature_412_residues
SDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEGY
RAGGFDANSCAVLVALDEQSPDISQGVNDADDINDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVRH
EEVLNYLLPDGKTQVSIDYEKGLPVSINTILISTQHNSEIDGVTNEEEIRQRIKEDLWKNVVIPATEDLEIKPNISKTRF
LVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYAI
GVAKPISILVETFDTGVISQNNLTELIKEHFDLRPAAIIKEFNLRNLPKKMGGKFFRKTASYGHFGRRDLELPWENVEEK
AAKLAEASKIFL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=397, Percent_Identity=54.4080604534005, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI5174529, Length=397, Percent_Identity=54.1561712846348, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1789311, Length=411, Percent_Identity=54.5012165450122, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551082, Length=411, Percent_Identity=54.014598540146, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25145633, Length=403, Percent_Identity=54.3424317617866, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538498, Length=414, Percent_Identity=53.1400966183575, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=400, Percent_Identity=53.75, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565905, Length=400, Percent_Identity=47, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25145635, Length=357, Percent_Identity=51.5406162464986, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=368, Percent_Identity=50.2717391304348, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=340, Percent_Identity=47.9411764705882, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=70.3703703703704, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=76.9230769230769, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=398, Percent_Identity=51.5075376884422, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6320710, Length=398, Percent_Identity=51.5075376884422, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=397, Percent_Identity=52.896725440806, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=397, Percent_Identity=52.896725440806, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=397, Percent_Identity=52.896725440806, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=397, Percent_Identity=52.896725440806, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=397, Percent_Identity=52.896725440806, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=397, Percent_Identity=52.896725440806, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=397, Percent_Identity=52.896725440806, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580525, Length=397, Percent_Identity=52.896725440806, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580541, Length=384, Percent_Identity=51.8229166666667, Blast_Score=337, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=58.8709677419355, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PROM0 (A3PB34)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090560.1
- ProteinModelPortal:   A3PB34
- SMR:   A3PB34
- STRING:   A3PB34
- GeneID:   4912479
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_03361
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   LHYEKAL
- ProtClustDB:   PRK05250
- BioCyc:   PMAR167546:P9301ORF_0341-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 45218; Mature: 45087

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSK
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AKVDYIKLVRNVIKEIGYEGYRAGGFDANSCAVLVALDEQSPDISQGVNDADDINDDLED
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHCCCCCCC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVRHEEVLNYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EKGLPVSINTILISTQHNSEIDGVTNEEEIRQRIKEDLWKNVVIPATEDLEIKPNISKTR
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEECCCCCCCEECCCCCCEE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVETFDTGVISQNNLTELIKEHFDLRPAAII
HHHHHHHHHHHHHHEEEEEHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHH
KEFNLRNLPKKMGGKFFRKTASYGHFGRRDLELPWENVEEKAAKLAEASKIFL
HHCCHHCCCHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSK
CCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AKVDYIKLVRNVIKEIGYEGYRAGGFDANSCAVLVALDEQSPDISQGVNDADDINDDLED
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHCCCCCCC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVRHEEVLNYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EKGLPVSINTILISTQHNSEIDGVTNEEEIRQRIKEDLWKNVVIPATEDLEIKPNISKTR
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEECCCCCCCEECCCCCCEE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVETFDTGVISQNNLTELIKEHFDLRPAAII
HHHHHHHHHHHHHHEEEEEHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHH
KEFNLRNLPKKMGGKFFRKTASYGHFGRRDLELPWENVEEKAAKLAEASKIFL
HHCCHHCCCHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA