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The map label for this gene is purH

Identifier: 126695627

GI number: 126695627

Start: 265622

End: 267175

Strand: Reverse

Name: purH

Synonym: P9301_02891

Alternate gene names: 126695627

Gene position: 267175-265622 (Counterclockwise)

Preceding gene: 126695629

Following gene: 126695622

Centisome position: 16.27

GC content: 32.63

Gene sequence:

>1554_bases
ATGTCTCCATTAGCTTTAGTAAGTGTCTCTGATAAAAAAAATATAATCCCATTTTGCAAGGAATTGATAGAGCAATTTAA
TTATAAAATTCTATCAAGTGGAGGAACTGCCAAACATCTTATAGATGCTAAGATTCCAGTTATTAAAGTTGCTGATTTTA
CAAATTCTCCAGAAATTCTTGGAGGAAGAGTTAAAACTTTACATCCAAAAATACACGGGGGAATATTAGCTAAAAGAACT
GATGAGGAACACAAAAAAGATGTAGAAACTAACAACCTTGAGTTAATTGACTTAGTAGTTGTCAATTTATATCCTTTTAA
AAAAACCGTAGATCAAGGAGCACAATGGGAAGATGCTATTGAAAATATCGATATCGGAGGGCCATCTATGATTCGTTCTG
CAGCTAAAAATCATAAAGATGTTTCTGTTTTAGTAGATCCTAGTCAGTATCAAAATTTTCTTGAAGAAAGTAAAAAAGGT
GAATTGAAAGACGCATATAAAGCAAAATTAGCCCTTGAAGCTTTTCAACATACAGCAGACTATGACACTGCAATATCTAA
TTGGATAAGAAAAGAAAGAGATTTACAATCTTCCAAATATATTGAATCTTATCCACTAATCAAAACCTTGAGATATGGGG
AGAATCCACATCAAAAAGCTTTTTGGTACGGTTTAAGTAACATTGGATGGAACTCAGCAGAACAATTACAAGGTAAAGAC
TTAAGTTATAACAATCTATTGGATCTAGAGTCGGCACTTTCAACAGTTTTAGAATTTGGCTACACAGAAAAAGATGAACT
TAAAACGGACATGTTTGCCTCCGTTATTTTAAAACACAATAATCCTTGTGGTGCCTCTATAAGTAATTCAGCTTCTAAAG
CATTTTTGAATGCCTTGGAATGTGACTCTGTTAGTGCATTCGGAGGAATAGTTGCTTTTAATTCAAATGTTGATAGTGAC
ACCGCTGTTCACCTCAAAGATATTTTCTTAGAGTGTGTCGTCGCTCCATCTTTTGATGAAGAAGCCTTAGAAATTTTAAA
AGTTAAAAAGAATTTAAGAATTTTAAAGTTTTCAAAAGATCAACTTCCAAAAAAGAATCAAAATTCTACTAAATCAATAA
TGGGAGGATTACTAGTTCAAGATACTGACGATAGTCAAGAAAAAACTGAGGATTGGATTTCAGTAACTAATAAAAATGCG
AATAATCAAGCTAACTTAGATCTAAATTTTGCATGGAAAATTTGTAAACACGTGAAATCTAATGCCATTGTTATTGCAAA
AGACCAAAAAACTATTGGTATTGGAGCTGGACAAATGAATAGAGTTGGAGCAGCAAAAATTGCATTAAAAGCAGCTGGAA
GTTTATGTTCTGATGCTGTCTTGGCTAGCGATGGGTTTTTCCCATTTGCAGATACTGTAGAACTAGCACACGAATATGGA
ATAAAAGCTATTATTCAACCTGGAGGAAGTCTAAGAGACCAAGAAAGTATTGATATGTGTAATTTGAAAGGAATATCAAT
GATATTTACCCAAAAAAGGCATTTTTTACATTAA

Upstream 100 bases:

>100_bases
AAATAATTCTATGAGTTGCAGTTTGAGAGCTAATCGAGACAAATTCATGATTGATAGCATATTTCATGTTTATATTCTTA
AGTAAGTAAACTTTCCCATA

Downstream 100 bases:

>100_bases
ATCATGTGGATTTTGGATAATATGTAATCTTATTAGTTTTTCTGAATAAAGATAACCGGCCTGGGCCTGCACATAGAAAG
TAGAGAGAAATAGCCCCATA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSPLALVSVSDKKNIIPFCKELIEQFNYKILSSGGTAKHLIDAKIPVIKVADFTNSPEILGGRVKTLHPKIHGGILAKRT
DEEHKKDVETNNLELIDLVVVNLYPFKKTVDQGAQWEDAIENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKG
ELKDAYKAKLALEAFQHTADYDTAISNWIRKERDLQSSKYIESYPLIKTLRYGENPHQKAFWYGLSNIGWNSAEQLQGKD
LSYNNLLDLESALSTVLEFGYTEKDELKTDMFASVILKHNNPCGASISNSASKAFLNALECDSVSAFGGIVAFNSNVDSD
TAVHLKDIFLECVVAPSFDEEALEILKVKKNLRILKFSKDQLPKKNQNSTKSIMGGLLVQDTDDSQEKTEDWISVTNKNA
NNQANLDLNFAWKICKHVKSNAIVIAKDQKTIGIGAGQMNRVGAAKIALKAAGSLCSDAVLASDGFFPFADTVELAHEYG
IKAIIQPGGSLRDQESIDMCNLKGISMIFTQKRHFLH

Sequences:

>Translated_517_residues
MSPLALVSVSDKKNIIPFCKELIEQFNYKILSSGGTAKHLIDAKIPVIKVADFTNSPEILGGRVKTLHPKIHGGILAKRT
DEEHKKDVETNNLELIDLVVVNLYPFKKTVDQGAQWEDAIENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKG
ELKDAYKAKLALEAFQHTADYDTAISNWIRKERDLQSSKYIESYPLIKTLRYGENPHQKAFWYGLSNIGWNSAEQLQGKD
LSYNNLLDLESALSTVLEFGYTEKDELKTDMFASVILKHNNPCGASISNSASKAFLNALECDSVSAFGGIVAFNSNVDSD
TAVHLKDIFLECVVAPSFDEEALEILKVKKNLRILKFSKDQLPKKNQNSTKSIMGGLLVQDTDDSQEKTEDWISVTNKNA
NNQANLDLNFAWKICKHVKSNAIVIAKDQKTIGIGAGQMNRVGAAKIALKAAGSLCSDAVLASDGFFPFADTVELAHEYG
IKAIIQPGGSLRDQESIDMCNLKGISMIFTQKRHFLH
>Mature_516_residues
SPLALVSVSDKKNIIPFCKELIEQFNYKILSSGGTAKHLIDAKIPVIKVADFTNSPEILGGRVKTLHPKIHGGILAKRTD
EEHKKDVETNNLELIDLVVVNLYPFKKTVDQGAQWEDAIENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKGE
LKDAYKAKLALEAFQHTADYDTAISNWIRKERDLQSSKYIESYPLIKTLRYGENPHQKAFWYGLSNIGWNSAEQLQGKDL
SYNNLLDLESALSTVLEFGYTEKDELKTDMFASVILKHNNPCGASISNSASKAFLNALECDSVSAFGGIVAFNSNVDSDT
AVHLKDIFLECVVAPSFDEEALEILKVKKNLRILKFSKDQLPKKNQNSTKSIMGGLLVQDTDDSQEKTEDWISVTNKNAN
NQANLDLNFAWKICKHVKSNAIVIAKDQKTIGIGAGQMNRVGAAKIALKAAGSLCSDAVLASDGFFPFADTVELAHEYGI
KAIIQPGGSLRDQESIDMCNLKGISMIFTQKRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=477, Percent_Identity=36.6876310272537, Blast_Score=239, Evalue=6e-63,
Organism=Escherichia coli, GI1790439, Length=536, Percent_Identity=45.1492537313433, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71985564, Length=474, Percent_Identity=36.0759493670886, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71985574, Length=304, Percent_Identity=27.6315789473684, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323768, Length=469, Percent_Identity=37.1002132196162, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=36.7864693446089, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24649832, Length=475, Percent_Identity=34.9473684210526, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PROM0 (A3PAY7)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090513.1
- STRING:   A3PAY7
- GeneID:   4911155
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_02891
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   PMAR167546:P9301ORF_0293-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 57215; Mature: 57083

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLALVSVSDKKNIIPFCKELIEQFNYKILSSGGTAKHLIDAKIPVIKVADFTNSPEIL
CCCEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCHHHEECCCCCEEEEECCCCCCHHH
GGRVKTLHPKIHGGILAKRTDEEHKKDVETNNLELIDLVVVNLYPFKKTVDQGAQWEDAI
CCCEEEECCHHHCCEEECCCCHHHHHCCCCCCEEEEEEEEEECCCCHHHHHCCCCHHHHH
ENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKGELKDAYKAKLALEAFQHTAD
HCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
YDTAISNWIRKERDLQSSKYIESYPLIKTLRYGENPHQKAFWYGLSNIGWNSAEQLQGKD
HHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCCCC
LSYNNLLDLESALSTVLEFGYTEKDELKTDMFASVILKHNNPCGASISNSASKAFLNALE
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
CDSVSAFGGIVAFNSNVDSDTAVHLKDIFLECVVAPSFDEEALEILKVKKNLRILKFSKD
CCCCHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECHH
QLPKKNQNSTKSIMGGLLVQDTDDSQEKTEDWISVTNKNANNQANLDLNFAWKICKHVKS
HCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCC
NAIVIAKDQKTIGIGAGQMNRVGAAKIALKAAGSLCSDAVLASDGFFPFADTVELAHEYG
CEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
IKAIIQPGGSLRDQESIDMCNLKGISMIFTQKRHFLH
CEEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SPLALVSVSDKKNIIPFCKELIEQFNYKILSSGGTAKHLIDAKIPVIKVADFTNSPEIL
CCEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCHHHEECCCCCEEEEECCCCCCHHH
GGRVKTLHPKIHGGILAKRTDEEHKKDVETNNLELIDLVVVNLYPFKKTVDQGAQWEDAI
CCCEEEECCHHHCCEEECCCCHHHHHCCCCCCEEEEEEEEEECCCCHHHHHCCCCHHHHH
ENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKGELKDAYKAKLALEAFQHTAD
HCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
YDTAISNWIRKERDLQSSKYIESYPLIKTLRYGENPHQKAFWYGLSNIGWNSAEQLQGKD
HHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCCCC
LSYNNLLDLESALSTVLEFGYTEKDELKTDMFASVILKHNNPCGASISNSASKAFLNALE
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
CDSVSAFGGIVAFNSNVDSDTAVHLKDIFLECVVAPSFDEEALEILKVKKNLRILKFSKD
CCCCHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECHH
QLPKKNQNSTKSIMGGLLVQDTDDSQEKTEDWISVTNKNANNQANLDLNFAWKICKHVKS
HCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCC
NAIVIAKDQKTIGIGAGQMNRVGAAKIALKAAGSLCSDAVLASDGFFPFADTVELAHEYG
CEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
IKAIIQPGGSLRDQESIDMCNLKGISMIFTQKRHFLH
CEEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA