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The map label for this gene is pyrB [H]

Identifier: 126695593

GI number: 126695593

Start: 237161

End: 238177

Strand: Reverse

Name: pyrB [H]

Synonym: P9301_02551

Alternate gene names: 126695593

Gene position: 238177-237161 (Counterclockwise)

Preceding gene: 126695594

Following gene: 126695590

Centisome position: 14.51

GC content: 32.84

Gene sequence:

>1017_bases
ATGCAAATTTGGCCTCATAAACATATCCACTCACTAGCTAATTTTTCAATAAAAGATTATGAGTCAGTATTCGAATTAGC
TAATAGATTTGATGCACTAAAGAATTCAGGAACGAAAAAAATACCAGCCTTACAGGGTACTTTAGTAACGTCTTTATTTT
TTGAACCTAGTACAAGAACAAAAAATAGTTTTGAGCTTGCAGCAAAAAGACTTTCTGCTGATGTCCAGACATTTGCCCCA
TCCTCTAGTTCTTTAACAAAAGGCGAAACAATAATTGATACTGCCATAACTTATTCTGCTATGGGAGCTGATACATTAGT
GATCAGACATTCATCAAGTTACATAACCTTTGAGATCGCTGAAAAACTTGATGCAATAAATTCCAAGACTTCGGTTCTTA
ATGCTGGAGATGGATTACATAGTCACCCTAGCCAGGGATTGCTTGACATATATACATTAATAAAATTCTTTTCCAAAAAT
ACACTGAATCCAGGGGTTTTAAATTCCAAAAAGATTTTAATAATTGGAGACGTTAATCATTCTAGGGTTGCCAGATCTAA
TCTTTGGGCTTTGAGTGCATTCGGCGCAGACATAATTTTATGCGGTCCTGAGACATTAATACCTGATGAATTTACCAATT
TTTTAAAAACTCCCGCGCCAAACCAAATAGAAGATCCTGTTAAATCAAGAGGATGCATAACAATTTCTAGATCTTTGGAA
GAATCAATAAAAATTGCAGATGCGATTATTGTTTTAAGACTTCAGAAAGAAAGAATGATGGAGAATTTACTCAGTAGCAT
CGATTCATATAGTTTGGATTATGGCTTAACCTCAGAGAAATTATCTTTGAATAATAAAGAAATTCCAATTCTTCATCCTG
GTCCAATTAACAGAGATATTGAAATAAGTAGCAAAGTAGTAGATCAATATCCTAATTGTTTAATTAATAATCAAGTTTCA
AATGGTATCCCTATAAGAATGGCTTTGCTTTATCTATTACAAAAAAATAACCAATAA

Upstream 100 bases:

>100_bases
TAAAATGGCGTTGGTATCTCAAAAATAGTAGGAGTGTAAGTAAAAGATTAAAAGGTGACAGAACACCTAAATTTCAATAA
TCATTTATCTTTTAAACGTA

Downstream 100 bases:

>100_bases
ATTTATAGCAACTTAAGACTTTCAGTAAAGAATCCATAAAATAATCCTAATCTTAAAGAATCTAAAAAAAGTATTAAAAA
TTTCTTTTTCCTTTCAGATC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQIWPHKHIHSLANFSIKDYESVFELANRFDALKNSGTKKIPALQGTLVTSLFFEPSTRTKNSFELAAKRLSADVQTFAP
SSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIAEKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSKN
TLNPGVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPETLIPDEFTNFLKTPAPNQIEDPVKSRGCITISRSLE
ESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTSEKLSLNNKEIPILHPGPINRDIEISSKVVDQYPNCLINNQVS
NGIPIRMALLYLLQKNNQ

Sequences:

>Translated_338_residues
MQIWPHKHIHSLANFSIKDYESVFELANRFDALKNSGTKKIPALQGTLVTSLFFEPSTRTKNSFELAAKRLSADVQTFAP
SSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIAEKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSKN
TLNPGVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPETLIPDEFTNFLKTPAPNQIEDPVKSRGCITISRSLE
ESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTSEKLSLNNKEIPILHPGPINRDIEISSKVVDQYPNCLINNQVS
NGIPIRMALLYLLQKNNQ
>Mature_338_residues
MQIWPHKHIHSLANFSIKDYESVFELANRFDALKNSGTKKIPALQGTLVTSLFFEPSTRTKNSFELAAKRLSADVQTFAP
SSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIAEKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSKN
TLNPGVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPETLIPDEFTNFLKTPAPNQIEDPVKSRGCITISRSLE
ESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTSEKLSLNNKEIPILHPGPINRDIEISSKVVDQYPNCLINNQVS
NGIPIRMALLYLLQKNNQ

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=328, Percent_Identity=29.8780487804878, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI2367364, Length=333, Percent_Identity=30.3303303303303, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790703, Length=321, Percent_Identity=27.1028037383178, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786469, Length=302, Percent_Identity=27.8145695364238, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=332, Percent_Identity=30.7228915662651, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322331, Length=333, Percent_Identity=27.6276276276276, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24642586, Length=334, Percent_Identity=30.5389221556886, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 37390; Mature: 37390

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIWPHKHIHSLANFSIKDYESVFELANRFDALKNSGTKKIPALQGTLVTSLFFEPSTRT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEECCCCCC
KNSFELAAKRLSADVQTFAPSSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEHH
EKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSKNTLNPGVLNSKKILIIGDVNH
HHHHHCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
SRVARSNLWALSAFGADIILCGPETLIPDEFTNFLKTPAPNQIEDPVKSRGCITISRSLE
HHHHHHCCEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEHHHHH
ESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTSEKLSLNNKEIPILHPGPINRDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
EISSKVVDQYPNCLINNQVSNGIPIRMALLYLLQKNNQ
CHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQIWPHKHIHSLANFSIKDYESVFELANRFDALKNSGTKKIPALQGTLVTSLFFEPSTRT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEECCCCCC
KNSFELAAKRLSADVQTFAPSSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEHH
EKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSKNTLNPGVLNSKKILIIGDVNH
HHHHHCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
SRVARSNLWALSAFGADIILCGPETLIPDEFTNFLKTPAPNQIEDPVKSRGCITISRSLE
HHHHHHCCEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEHHHHH
ESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTSEKLSLNNKEIPILHPGPINRDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
EISSKVVDQYPNCLINNQVSNGIPIRMALLYLLQKNNQ
CHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA