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The map label for this gene is rnhA [H]

Identifier: 126695558

GI number: 126695558

Start: 205070

End: 205789

Strand: Reverse

Name: rnhA [H]

Synonym: P9301_02201

Alternate gene names: 126695558

Gene position: 205789-205070 (Counterclockwise)

Preceding gene: 126695559

Following gene: 126695557

Centisome position: 12.53

GC content: 29.31

Gene sequence:

>720_bases
ATGAATATTGATTGTATTGCAATTGAAGCTGCAACAGATGGAGCCTGCAGTGGTAATCCAGGTCCAGGAGGTTGGGGTGG
TTTAATAATTTTTGATGACAAAAGTGAATTAGAAATAGGTGGTTCCGAGCAAAATACTACTAATAATAGAATGGAACTAA
CTGCGGCTATAAAAACACTTGAGAAATTAAAAACCTTCAAATTAAAAGAAAACTTTAAATTAAGAACTGATAGTAAATAT
GTAATAGAAGGGTATACAAAATGGATTATTAATTGGAAGAAGAATGGATGGAAAACAAGCTCAGGAAAATCAGTTCAGAA
TCTTGATTTATGGCAAAAAATTGATCAATTAAGAATTAATGGTCTTGTAATGGAATATGTTAAAGGCCATAGCGGTGACA
AACAAAATGATAGGGTAGATAAAATCGCGACCAACTATAGCAAAGGTATATCTCTAAAAAGTAACTTAAAAGAGTCTGAA
TCTTCAGTAGAATTTTTTGAAAAAAATGCACCTTCAGAAATTCAGGAATTATTTTCAAGAAATGAATTAATTCAAAAATT
TGCTGACAAAAAGTACTTGTTAAATTCACTAGAACTAAGTAATTTATTAGGTGAAGAAAACCAGATAAATATAAAACAAT
ATTTACTTTTTGAATGGCGTAATTGGAGATTGATTCCCAAAGATAAAAAATATTGGATAATAGAAAAAAAAGAATTCTAA

Upstream 100 bases:

>100_bases
AATCTTTAAAGAAAGAGATTGAAGAAGCTGGCGGTAAAGTTACACTTAAGTAAGAGAATATTTTTTCAAGCCGATAGACT
TTATATCGGCTTCAAAAATT

Downstream 100 bases:

>100_bases
TTTTTTATGAATGAACAGCAGGGGGTATTTAAAAATCTTTATAAAAACTTGATGACACCTATACTAAAAAATGATTCTGG
AATAGATGCAGAATATTTAA

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNIDCIAIEAATDGACSGNPGPGGWGGLIIFDDKSELEIGGSEQNTTNNRMELTAAIKTLEKLKTFKLKENFKLRTDSKY
VIEGYTKWIINWKKNGWKTSSGKSVQNLDLWQKIDQLRINGLVMEYVKGHSGDKQNDRVDKIATNYSKGISLKSNLKESE
SSVEFFEKNAPSEIQELFSRNELIQKFADKKYLLNSLELSNLLGEENQINIKQYLLFEWRNWRLIPKDKKYWIIEKKEF

Sequences:

>Translated_239_residues
MNIDCIAIEAATDGACSGNPGPGGWGGLIIFDDKSELEIGGSEQNTTNNRMELTAAIKTLEKLKTFKLKENFKLRTDSKY
VIEGYTKWIINWKKNGWKTSSGKSVQNLDLWQKIDQLRINGLVMEYVKGHSGDKQNDRVDKIATNYSKGISLKSNLKESE
SSVEFFEKNAPSEIQELFSRNELIQKFADKKYLLNSLELSNLLGEENQINIKQYLLFEWRNWRLIPKDKKYWIIEKKEF
>Mature_239_residues
MNIDCIAIEAATDGACSGNPGPGGWGGLIIFDDKSELEIGGSEQNTTNNRMELTAAIKTLEKLKTFKLKENFKLRTDSKY
VIEGYTKWIINWKKNGWKTSSGKSVQNLDLWQKIDQLRINGLVMEYVKGHSGDKQNDRVDKIATNYSKGISLKSNLKESE
SSVEFFEKNAPSEIQELFSRNELIQKFADKKYLLNSLELSNLLGEENQINIKQYLLFEWRNWRLIPKDKKYWIIEKKEF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=141, Percent_Identity=36.1702127659575, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1786408, Length=159, Percent_Identity=43.3962264150943, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532542, Length=136, Percent_Identity=39.7058823529412, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17505482, Length=129, Percent_Identity=37.984496124031, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 27571; Mature: 27571

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDCIAIEAATDGACSGNPGPGGWGGLIIFDDKSELEIGGSEQNTTNNRMELTAAIKTL
CCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHH
EKLKTFKLKENFKLRTDSKYVIEGYTKWIINWKKNGWKTSSGKSVQNLDLWQKIDQLRIN
HHHHHHHHCCCCEEECCCEEEEECEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GLVMEYVKGHSGDKQNDRVDKIATNYSKGISLKSNLKESESSVEFFEKNAPSEIQELFSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NELIQKFADKKYLLNSLELSNLLGEENQINIKQYLLFEWRNWRLIPKDKKYWIIEKKEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCEECCCCCEEEEEEECCC
>Mature Secondary Structure
MNIDCIAIEAATDGACSGNPGPGGWGGLIIFDDKSELEIGGSEQNTTNNRMELTAAIKTL
CCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHH
EKLKTFKLKENFKLRTDSKYVIEGYTKWIINWKKNGWKTSSGKSVQNLDLWQKIDQLRIN
HHHHHHHHCCCCEEECCCEEEEECEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GLVMEYVKGHSGDKQNDRVDKIATNYSKGISLKSNLKESESSVEFFEKNAPSEIQELFSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NELIQKFADKKYLLNSLELSNLLGEENQINIKQYLLFEWRNWRLIPKDKKYWIIEKKEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]