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The map label for this gene is menE [H]

Identifier: 126695532

GI number: 126695532

Start: 178439

End: 179641

Strand: Reverse

Name: menE [H]

Synonym: P9301_01941

Alternate gene names: 126695532

Gene position: 179641-178439 (Counterclockwise)

Preceding gene: 126695533

Following gene: 126695529

Centisome position: 10.94

GC content: 26.6

Gene sequence:

>1203_bases
ATGAAAAATAAAATTCATACTATTGAAGTTGAAAATAACGAAACTCAATCTGTAGAAAAAATTATTGAAAAAATTAGAGA
TAATAAAATTATTTATGTAAAAAACAAATTTTATGAAGACAAAGATGTATTAGATTCAATTACTGAAAATGGGCCAGCAA
TTATCTTAAACAGTAGTGGGAGTTCTGGAAAACCGAGACAATGTTTTCACCATTTAAATAATCTTAAATTATCTGCAACT
ACATCAGGTCAATGGCTAATAGAGCAAGGATTTGAATTACAAAACTGCTTAATTTTAAATACTTTACCCCTGAACCATAT
AAGTGGATTAATGCCAATTTTTAGAAGTCAAATTTGGGGATGTGATTGTATTAATATTTCTCCGAATTTGATAAAAAAAA
CTCGAGAACTTTTACTTTTCACAATTAAATTTAAAAAAAACAAAAAACACTTGATTACATCATTAGTACCTACCCAATTA
CAAAGACTTTTAGCTCAAAAAGATGGAATTAGTTGGCTAAAAATTTTTGATCTAATTTGGGTAGGTGGAGCTTCAATTTC
AGACGAAACGGCAGAACAATGTATAAAAGAAAAAATAAAATTAGCACCTTGTTACGGCGCAACTGAAACAGCAGCAATGG
TTACGAGTTTAAAACCAAAAGAATTCTTAATGGGTTTTAAAAATGTTGGAGAAATACTACCTGATACAAAAATAAGAATT
AACGCACAAGGATTAATCGAAATAAAATCAGCCAGAATTGGAATCGAAATAATAGATTCTTTAAAAACTGAAAATTTCAA
AAATAAAAATGGGTGGTGGCAAACAAGTGATTTAGGTGAAATTAAGCAAATCAATAATTCTTACTATTTAAAATTTGTTG
GAAGAAGTGATAATGCCTTTAATTCAGGAGGAGAAGTTGTTTTCCCTGAAGTAATTGAATCTAGATTAAATGATTTCATT
ATGAAAGAAAATATCCCAATTAATAAATTCAATATTGCGAAAGTATCTAACAAATTATGGGGAAATAAAATTAAAGTAAT
AGTTGAATTTAGAGAACTTACAAATGATAAAAATATTGAAATTTCTTTAAATTTATTAAAAAATTTTTCTCAAAGTTGGC
CTAAACATGAAAAGCCTGAAAAGTGGATTGTTAAAAACAAAAATAGCATTACAGAAAAAATAAACTATAAATTTAAAAAA
TAA

Upstream 100 bases:

>100_bases
AATTACAAGGACCAACACCAAAAGTTCCTGGTTTAGCAATGAATAAATTCCCTAATTCGTTTCTATTTTTAAATGAAGCA
AAAACGATATGGGATCAACT

Downstream 100 bases:

>100_bases
TATTCAGCATTCTTTTAAATTTGTACTCACATTAGAAGCCTCTATCCATCTTTCTAACTGATCGGGAATTTCTATTTTAT
TTCTTGAATCAACATCTAAA

Product: putative O-succinylbenzoic acid--CoA ligase (OSB-CoA synthetase)

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKNKIHTIEVENNETQSVEKIIEKIRDNKIIYVKNKFYEDKDVLDSITENGPAIILNSSGSSGKPRQCFHHLNNLKLSAT
TSGQWLIEQGFELQNCLILNTLPLNHISGLMPIFRSQIWGCDCINISPNLIKKTRELLLFTIKFKKNKKHLITSLVPTQL
QRLLAQKDGISWLKIFDLIWVGGASISDETAEQCIKEKIKLAPCYGATETAAMVTSLKPKEFLMGFKNVGEILPDTKIRI
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTSDLGEIKQINNSYYLKFVGRSDNAFNSGGEVVFPEVIESRLNDFI
MKENIPINKFNIAKVSNKLWGNKIKVIVEFRELTNDKNIEISLNLLKNFSQSWPKHEKPEKWIVKNKNSITEKINYKFKK

Sequences:

>Translated_400_residues
MKNKIHTIEVENNETQSVEKIIEKIRDNKIIYVKNKFYEDKDVLDSITENGPAIILNSSGSSGKPRQCFHHLNNLKLSAT
TSGQWLIEQGFELQNCLILNTLPLNHISGLMPIFRSQIWGCDCINISPNLIKKTRELLLFTIKFKKNKKHLITSLVPTQL
QRLLAQKDGISWLKIFDLIWVGGASISDETAEQCIKEKIKLAPCYGATETAAMVTSLKPKEFLMGFKNVGEILPDTKIRI
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTSDLGEIKQINNSYYLKFVGRSDNAFNSGGEVVFPEVIESRLNDFI
MKENIPINKFNIAKVSNKLWGNKIKVIVEFRELTNDKNIEISLNLLKNFSQSWPKHEKPEKWIVKNKNSITEKINYKFKK
>Mature_400_residues
MKNKIHTIEVENNETQSVEKIIEKIRDNKIIYVKNKFYEDKDVLDSITENGPAIILNSSGSSGKPRQCFHHLNNLKLSAT
TSGQWLIEQGFELQNCLILNTLPLNHISGLMPIFRSQIWGCDCINISPNLIKKTRELLLFTIKFKKNKKHLITSLVPTQL
QRLLAQKDGISWLKIFDLIWVGGASISDETAEQCIKEKIKLAPCYGATETAAMVTSLKPKEFLMGFKNVGEILPDTKIRI
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTSDLGEIKQINNSYYLKFVGRSDNAFNSGGEVVFPEVIESRLNDFI
MKENIPINKFNIAKVSNKLWGNKIKVIVEFRELTNDKNIEISLNLLKNFSQSWPKHEKPEKWIVKNKNSITEKINYKFKK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=313, Percent_Identity=26.8370607028754, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=361, Percent_Identity=24.6537396121884, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 45825; Mature: 45825

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKIHTIEVENNETQSVEKIIEKIRDNKIIYVKNKFYEDKDVLDSITENGPAIILNSSG
CCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCC
SSGKPRQCFHHLNNLKLSATTSGQWLIEQGFELQNCLILNTLPLNHISGLMPIFRSQIWG
CCCCHHHHHHHHCCEEEEEECCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
CDCINISPNLIKKTRELLLFTIKFKKNKKHLITSLVPTQLQRLLAQKDGISWLKIFDLIW
CEEECCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGGASISDETAEQCIKEKIKLAPCYGATETAAMVTSLKPKEFLMGFKNVGEILPDTKIRI
CCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTSDLGEIKQINNSYYLKFVGRSDNAF
ECCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEECCCCHHHHHCCCCEEEEEEECCCCCC
NSGGEVVFPEVIESRLNDFIMKENIPINKFNIAKVSNKLWGNKIKVIVEFRELTNDKNIE
CCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCEEEEEEEEHHHCCCCCEE
ISLNLLKNFSQSWPKHEKPEKWIVKNKNSITEKINYKFKK
EEHHHHHHHHHHCCCCCCCCHHEEECCCHHHHHCCCEECC
>Mature Secondary Structure
MKNKIHTIEVENNETQSVEKIIEKIRDNKIIYVKNKFYEDKDVLDSITENGPAIILNSSG
CCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCC
SSGKPRQCFHHLNNLKLSATTSGQWLIEQGFELQNCLILNTLPLNHISGLMPIFRSQIWG
CCCCHHHHHHHHCCEEEEEECCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
CDCINISPNLIKKTRELLLFTIKFKKNKKHLITSLVPTQLQRLLAQKDGISWLKIFDLIW
CEEECCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGGASISDETAEQCIKEKIKLAPCYGATETAAMVTSLKPKEFLMGFKNVGEILPDTKIRI
CCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTSDLGEIKQINNSYYLKFVGRSDNAF
ECCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEECCCCHHHHHCCCCEEEEEEECCCCCC
NSGGEVVFPEVIESRLNDFIMKENIPINKFNIAKVSNKLWGNKIKVIVEFRELTNDKNIE
CCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCEEEEEEEEHHHCCCCCEE
ISLNLLKNFSQSWPKHEKPEKWIVKNKNSITEKINYKFKK
EEHHHHHHHHHHCCCCCCCCHHEEECCCHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA