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The map label for this gene is cls [H]

Identifier: 126701027

GI number: 126701027

Start: 3982188

End: 3983669

Strand: Direct

Name: cls [H]

Synonym: CD3404

Alternate gene names: 126701027

Gene position: 3982188-3983669 (Clockwise)

Preceding gene: 126700990

Following gene: 126701028

Centisome position: 92.82

GC content: 28.14

Gene sequence:

>1482_bases
ATGTTTGATATATCATTTTTCGAAATGTCAATATATGAAATAGCAGTTACTACAATATATATAATTAATTTCATGGTTAT
ACTAAACCTTATCTTTAGAGAAAAAAGAAACATAAATACAACTTTAACATGGCTTCTTATATTAGTATTGATTCCTGCTC
TTGGATTCATACTTTATATAGCTTTTGGCAGAAATATTTCAAAAAACAATATGTTTAGATTGAAAGAAAAGGATGATAAG
ATAATCAAAAGTAATATACTAGATACTCAGGTTAAACTCCAATCTACATCTGAAATTGATTCAGATATACATCAACATAA
GGATATGATTTATGCATTGGCTAATTCAAATAATGCTCATTATACCAATAATAACGATGTGTGGATTTATGCTGAATCTA
GTCAATTTTTTAACAGTCTTCTTGAAGAACTTAAAAAAGCTAAAAAATATATAAACATACAATTTTACATTTTCAAAGAT
GACAAAATCGGAACTGAAATAATAGACATTCTTATAGACAAAGCTAAAGAAGGTGTTGAAGTAAGACTTTTATTTGATGC
AGTTGGTGGTAGAACACTAAAAAATAGTACACTTTCCAGACTTAAAGAAAGTGGTGTTAAAGTTGGAAGCTTCTTCCCAT
CATTCTTGAAAATTGTAAACTTTAACTTAAACTATAGAAATCACCGAAAAATTGTTGTCATTGATGGTAAGGTAGGATTT
GTAGGTGGATATAATGTTGGTGATGAATATTTAGGTAGAAATCCAAAATTTGGTCTATGGAGAGATACACATACTAAGCT
TACTGGTGATTGCGTAATTGACCTAAATATGAGATTCATCCTTGATTGGAGATATACAACTAAAGAAGACTTAGATTTAG
AAAAGTACTTTATAGAAGAAGATACAAATTCTAATCATCCTTCTGAAAATATTGGGATTCAGATAGTATCAAGTGGTCCA
GATATATCAGAACTTGATGAGATTAAATATGGTTACCTTAAAATGATTCAGAAAGCTAGAAAGTATATATACATACAGAG
TCCTTATTTAATACTTGACTCAACTTTTATAGATACTTTAAAAATAGCTTGTTTATCTGGTGTTGATGTGAGAGTTATGA
TTCCTTCTAAACCTGACCATCCCTTTGTATACTGGGCATCATATTCATATGCTGGAGAACTTTTAAAGTTTGGAGCTAAA
ATCTATACTTATGGTCAAAATGCATTTTTACATGCTAAGACAATAGTTATAGATGACTCTATATGCTCTATAGGAACAGC
TAATATGGATATCAGAAGTTTTGAGCTTAATTTCGAAGTTAATGCATTTATGTACTCTTCAAAAAAAGCATTGGAACAAA
GAATTATATTTGAAAATGATATTTTAAATTCTAAGGAAATAACATTAGATATATATAATTCTAGGTCTACTTATATAAAA
ATCAAGGAATCAATTTCAAGACTATTATCAGCAGTTTTATAA

Upstream 100 bases:

>100_bases
TTTTACTTTTTAATTATTGAGGTATTATATATAATAGATATATGTTTGTGTTATTATATATAATTACTTATTTTAATAAA
TATTTGAAGGAGGGAATTTT

Downstream 100 bases:

>100_bases
ATCTTTTATTTTATAAAAATTTTACATTTTGGTTTATTTTTTAAAGTTTTTTATATGTATTATTTTTTTTTTATTGAATT
TAGCTCATTTTTTTTATTTT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDK
IIKSNILDTQVKLQSTSEIDSDIHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYRNHRKIVVIDGKVGF
VGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGP
DISELDEIKYGYLKMIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAK
IYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKALEQRIIFENDILNSKEITLDIYNSRSTYIK
IKESISRLLSAVL

Sequences:

>Translated_493_residues
MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDK
IIKSNILDTQVKLQSTSEIDSDIHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYRNHRKIVVIDGKVGF
VGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGP
DISELDEIKYGYLKMIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAK
IYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKALEQRIIFENDILNSKEITLDIYNSRSTYIK
IKESISRLLSAVL
>Mature_493_residues
MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDK
IIKSNILDTQVKLQSTSEIDSDIHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYRNHRKIVVIDGKVGF
VGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGP
DISELDEIKYGYLKMIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAK
IYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKALEQRIIFENDILNSKEITLDIYNSRSTYIK
IKESISRLLSAVL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=510, Percent_Identity=23.5294117647059, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787007, Length=341, Percent_Identity=26.099706744868, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56889; Mature: 56889

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIPALGFILYI
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSDIHQHKDMIYALANSNNAH
HHCCCCCCCCEEEEECCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHCEEEEEECCCCCE
YTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKDDKIGTEIIDILIDKAKEGVE
EECCCCEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHCCCCCCE
VRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYRNHRKIVVIDGKVGF
EEEEEECCCCCEEHHHHHHHHHHCCCEEHHHHHHHHHHEEECCCCCCCCEEEEEECCEEE
VGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWRYTTKEDLDLEKYFIEE
EECCCCCHHHCCCCCCCCCCCCCCCEEECCEEEEECEEEEEEEECCCCCCCCHHHEEEEC
DTNSNHPSENIGIQIVSSGPDISELDEIKYGYLKMIQKARKYIYIQSPYLILDSTFIDTL
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECHHHHHH
KIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAKIYTYGQNAFLHAKTIVIDDS
HHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHCCCEEEEECCCEEEEEEEEEEECC
ICSIGTANMDIRSFELNFEVNAFMYSSKKALEQRIIFENDILNSKEITLDIYNSRSTYIK
HHCCCCCCCEEEEEEEEEEEEEEEECCHHHHHHHHHECCCCCCCCEEEEEEECCCCEEEE
IKESISRLLSAVL
EHHHHHHHHHHHC
>Mature Secondary Structure
MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTWLLILVLIPALGFILYI
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSDIHQHKDMIYALANSNNAH
HHCCCCCCCCEEEEECCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHCEEEEEECCCCCE
YTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKDDKIGTEIIDILIDKAKEGVE
EECCCCEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHCCCCCCE
VRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKIVNFNLNYRNHRKIVVIDGKVGF
EEEEEECCCCCEEHHHHHHHHHHCCCEEHHHHHHHHHHEEECCCCCCCCEEEEEECCEEE
VGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLNMRFILDWRYTTKEDLDLEKYFIEE
EECCCCCHHHCCCCCCCCCCCCCCCEEECCEEEEECEEEEEEEECCCCCCCCHHHEEEEC
DTNSNHPSENIGIQIVSSGPDISELDEIKYGYLKMIQKARKYIYIQSPYLILDSTFIDTL
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECHHHHHH
KIACLSGVDVRVMIPSKPDHPFVYWASYSYAGELLKFGAKIYTYGQNAFLHAKTIVIDDS
HHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHCCCEEEEECCCEEEEEEEEEEECC
ICSIGTANMDIRSFELNFEVNAFMYSSKKALEQRIIFENDILNSKEITLDIYNSRSTYIK
HHCCCCCCCEEEEEEEEEEEEEEEECCHHHHHHHHHECCCCCCCCEEEEEEECCCCEEEE
IKESISRLLSAVL
EHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]