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The map label for this gene is ansA [H]

Identifier: 126700132

GI number: 126700132

Start: 2905443

End: 2906438

Strand: Direct

Name: ansA [H]

Synonym: CD2516

Alternate gene names: 126700132

Gene position: 2905443-2906438 (Clockwise)

Preceding gene: 126700131

Following gene: 126700136

Centisome position: 67.72

GC content: 32.53

Gene sequence:

>996_bases
ATGAGTATTAAGAAAAAAGTTGCCATAGTTTTTACTGGTGGAACTATATCTATGACAGTAGATGATAAAGTCGGAGCAGC
TATACCAACTTTATCAGGTGAACAAATACTATCAATGGTTACCAACATTGATAAAGTTGCAGATGTTGAAGTTTTTAATT
TTGATGAAATTCCAGGACCTCACATAACACCTTATAGAATGATGGAATTGAAAAACTATGTAAATGATTTATTAGCAAGA
AAAGATATTACAGGTGTTGTAATAACACATGGTACAGATAGTTTAGAGGAAACAGCTTATTTCCTAGACCTTACAATTGA
CAACATCAAACCTGTAATAGTCACAGGTGCTATGAGAAGTAGTTCTGAACTTGGATATGATGGTTCAAGTAATTTATCTG
CATCAGTTTGTACTGCAGTATCCAAAGATGCTATGGGAAAAGGAGTCCTTGTAGTTTTAAACAATGAAGTATTATTAGCT
TCTGAAGCTACTAAAACAAATACTCTTTCTCTAAATACATTTAAATCTCTAACTAGTGGTCCATTGGGAATAATTGACTG
CAACGAACTTGTCCTTACAAGAGATATTGTAAATAGAACAATTATAGATACTGATAAGGTAGAATCTAAAGTTGCCTTAT
TTAAAGCATATGTAGGAGAAAATGCTGACTTTATAAGATTTGCTGTTGATAGTGGTTATAAAGGTATAGTTATTGAGGCA
ATGGGTAGAGGTAATATTCCTCCTCAAATGCTTTCTGGAGTTGAATATGCTAGAGAAAAAGGACTTCCTGTAGTTATAGT
TTCTAGATGTCATTCAGGTAGAGTATTCGATAGTTATGGTTACTTTGGTTCTGGTAGAGATTTAAAAAATCTTGGTTGTA
TATTTGGTGGAGACTTACCAGGTCAAAAAGCTAGAATAAAACTTATCTTAGCACTTGGAAAAACTGATAACCTTGATGAA
ATCAAAGACTTCTTTGAAAAAGGAATCTATTGTTAA

Upstream 100 bases:

>100_bases
TAAATTATATGAATAAAAGAGTTTATGTAGACCTTAACTACTTTTTTTGATATAATCCTATCGGAATTATAAATTTTATT
TATGAAAGGCAGAGAATAGT

Downstream 100 bases:

>100_bases
TTCTAATAACTTCTATTTAATTATTTATTGATATTTCAACATTTTTAAATCAAAATCTAATACTTATGTCAGTTAAATGT
CAGCAAAATATTTTATCCAC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNIDKVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLAR
KDITGVVITHGTDSLEETAYFLDLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYVGENADFIRFAVDSGYKGIVIEA
MGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVFDSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDE
IKDFFEKGIYC

Sequences:

>Translated_331_residues
MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNIDKVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLAR
KDITGVVITHGTDSLEETAYFLDLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYVGENADFIRFAVDSGYKGIVIEA
MGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVFDSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDE
IKDFFEKGIYC
>Mature_330_residues
SIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNIDKVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARK
DITGVVITHGTDSLEETAYFLDLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLAS
EATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYVGENADFIRFAVDSGYKGIVIEAM
GRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVFDSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEI
KDFFEKGIYC

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=252, Percent_Identity=28.1746031746032, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1789327, Length=249, Percent_Identity=38.1526104417671, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI193207372, Length=346, Percent_Identity=25.4335260115607, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558278, Length=346, Percent_Identity=25.4335260115607, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558280, Length=346, Percent_Identity=25.4335260115607, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193207374, Length=346, Percent_Identity=25.4335260115607, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=341, Percent_Identity=30.2052785923754, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323187, Length=341, Percent_Identity=30.2052785923754, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323186, Length=341, Percent_Identity=30.2052785923754, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323184, Length=341, Percent_Identity=30.2052785923754, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320527, Length=337, Percent_Identity=31.7507418397626, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645530, Length=346, Percent_Identity=25.7225433526012, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639609, Length=362, Percent_Identity=23.7569060773481, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35773; Mature: 35641

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNIDKVADVEVFNFDEIPGP
CCCCCEEEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCC
HITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFLDLTIDNIKPVIVTGAMRS
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCEEEEEEEECCCCEEEEEECCCC
SSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLASEATKTNTLSLNTFKSLTSG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCEEEEHHHHHHCCC
PLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYVGENADFIRFAVDSGYKGIVIEA
CEEEEECCHHEEEHHHHHCEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEE
MGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVFDSYGYFGSGRDLKNLGCIFGGDLP
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCEEECCCCC
GQKARIKLILALGKTDNLDEIKDFFEKGIYC
CCCEEEEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNIDKVADVEVFNFDEIPGP
CCCCEEEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCC
HITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFLDLTIDNIKPVIVTGAMRS
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCEEEEEEEECCCCEEEEEECCCC
SSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLASEATKTNTLSLNTFKSLTSG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCEEEEHHHHHHCCC
PLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYVGENADFIRFAVDSGYKGIVIEA
CEEEEECCHHEEEHHHHHCEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEE
MGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVFDSYGYFGSGRDLKNLGCIFGGDLP
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCEEECCCCC
GQKARIKLILALGKTDNLDEIKDFFEKGIYC
CCCEEEEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]