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The map label for this gene is sir1 [H]

Identifier: 126700047

GI number: 126700047

Start: 2806465

End: 2808012

Strand: Direct

Name: sir1 [H]

Synonym: CD2431

Alternate gene names: 126700047

Gene position: 2806465-2808012 (Clockwise)

Preceding gene: 126700023

Following gene: 126700073

Centisome position: 65.41

GC content: 27.97

Gene sequence:

>1548_bases
ATGGATAATTTTAAAAAGATATTGTTAAATGAACTACCTAATTTTAAAGAATATTCTTCAAAATTTTTAAATGGAGAAAT
AAATAAAATGCAATATAAGGGATTTTCTGGTGGATATGGTGTTTATGCCCAAAGAGACAAAAAATCTTTTATGATAAGAC
TTAGAGTATCTGCTGGAGTTTTATCTCAGTATCAACTTAATAAAATCTATGAAATAGCTATTAAACACAACTTAGATAAG
ATACACTTAACAACTAGACAAGCTATACAATTACACGATTTATCTATAAATTCAATTGTTGATATCATGGAAGAAGGAAT
AAAAAATGATATATTCACTAGAGGTGGTGGTGGTAATTATCCTAGAAATGTCGGATTATCTCCATTATCTGGAGTTGACC
CTAGTGAAGTCTTTGATGTTACTCCATATGCAGTTGCTACTGATAAATATTTTATCAAAAATGCTACTACATACCATTTA
CCTAGAAAATTAAAAGTTTCTTATTCTAATTGTTACACAGATACTGCTCATTGTAGTATACAAGACTTAGGATTTGTTGC
TACTTTGAAAAATGATGAACCATATTTCAGAGTTTTCTTAGGTGGAGGTCTAGGTAAACAACCCAAAGTTGCTTTAGAAC
TTGATGAACTTATAAAGCCGAAAGATGCCTTATATTGTGTTGAAGGTATGATTAAATTCTTTATGGATTATGGAAATTAT
GAAAATAAAAACAGAGCTAGGGTTAGATATATGGTCGAATCACTTGGTGAAGAACTATTCTTAGAAAAATTTAAAGAATA
CTATAAATTAGAAAAAGAAAATGGTAGTCTTGAACTCAATATTGAAGAAATAGATTACTCTAAACCTGGAGTTAAAATAG
ATATACAAGATTCTAGACTAATACCTCAAAAACAGGATGGTTTATACACTGTATATATACATCCAGTTGGAGGTATTTTG
TTAACAAAGGATTTAAGTACTTTACTTAAAGAATTAGATAATGTTGAAAATCCTATGATTAGACTAGGAATGACAGAGGG
ATTATATATTTTAAATTTAAATGGAAATGAAGCAAAAAGAATATTAGAAATAAGTAAAACTATTAGTTGTAATTCTCAAT
TAGAACAAAGTATATCTTGCATAGGTGTTCCAATTTGTCAAATGGGCATACAAAATAGTCAGAAAATGTTACATGAAATC
ATAGATTATTTTAGATTACAAAATAATGAAGAAATTTTAAATAAAGCACCTAAGTTATATATATCAGGATGTTTAAATTC
ATGTGGTGTTCATCAAGTTGGTAGCATAGGTTTATGTGGTAAAAAGAAGAATGTTGATGGTATATCTACAGATGCATTTG
AATTATTTGTTGGAGGCAGTTTTGAAATAGGTAAGACTAGATTGGGAAAATCACTTGGAGATTTTAAAGCTAGTGATATT
CCTGAAATGCTTTATAAAATATCGGACGCATCATCAGGCAATTTTTATGAATGGGTAAATTCAAATGATAATATTTTAAA
CAAAATAACTGATAAGTATAAAATATAA

Upstream 100 bases:

>100_bases
AGAATTATTCTGTTATATTTTTATCCTGACTAGATAATAATATATATATCTATTATACAATAGATATATTTTTATACAAG
ACTAACAATGAGGTGAAATA

Downstream 100 bases:

>100_bases
TTTATTATATTTACTTACAAAAAACATGGGTGTCTCAAAATGAACTTTTTAGTTTATGAGACACTCTTTTTCTATGAAAT
CAAATATATTTTCATCATCT

Product: nitrite/sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQRDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDK
IHLTTRQAIQLHDLSINSIVDIMEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELIKPKDALYCVEGMIKFFMDYGNY
ENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGSLELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGIL
LTKDLSTLLKELDNVENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQNSQKMLHEI
IDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGISTDAFELFVGGSFEIGKTRLGKSLGDFKASDI
PEMLYKISDASSGNFYEWVNSNDNILNKITDKYKI

Sequences:

>Translated_515_residues
MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQRDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDK
IHLTTRQAIQLHDLSINSIVDIMEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELIKPKDALYCVEGMIKFFMDYGNY
ENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGSLELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGIL
LTKDLSTLLKELDNVENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQNSQKMLHEI
IDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGISTDAFELFVGGSFEIGKTRLGKSLGDFKASDI
PEMLYKISDASSGNFYEWVNSNDNILNKITDKYKI
>Mature_515_residues
MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQRDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDK
IHLTTRQAIQLHDLSINSIVDIMEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELIKPKDALYCVEGMIKFFMDYGNY
ENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGSLELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGIL
LTKDLSTLLKELDNVENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQNSQKMLHEI
IDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGISTDAFELFVGGSFEIGKTRLGKSLGDFKASDI
PEMLYKISDASSGNFYEWVNSNDNILNKITDKYKI

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=538, Percent_Identity=21.7472118959108, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 58209; Mature: 58209

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQRDKKSFMIRLRVSAGV
CCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCEEEEEEEECCCC
LSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDIMEEGIKNDIFTRGGGGNY
HHHHHHHHHEEEHHHCCCCEEEEEHHHEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCC
PRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHLPRKLKVSYSNCYTDTAHCSI
CCCCCCCCCCCCCHHHHEECCCCEEECCCEEEECCEEEECCCEEEEEEHHCCCCCCCCCC
QDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELIKPKDALYCVEGMIKFFMDYGNY
CCCCEEEEECCCCCEEEEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCC
ENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGSLELNIEEIDYSKPGVKIDIQDSRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCCEEEEECCCCC
IPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDNVENPMIRLGMTEGLYILNLNGNEAKR
CCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHH
ILEISKTISCNSQLEQSISCIGVPICQMGIQNSQKMLHEIIDYFRLQNNEEILNKAPKLY
HHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCEE
ISGCLNSCGVHQVGSIGLCGKKKNVDGISTDAFELFVGGSFEIGKTRLGKSLGDFKASDI
EEHHHCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCH
PEMLYKISDASSGNFYEWVNSNDNILNKITDKYKI
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQRDKKSFMIRLRVSAGV
CCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCEEEEEEEECCCC
LSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDIMEEGIKNDIFTRGGGGNY
HHHHHHHHHEEEHHHCCCCEEEEEHHHEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCC
PRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHLPRKLKVSYSNCYTDTAHCSI
CCCCCCCCCCCCCHHHHEECCCCEEECCCEEEECCEEEECCCEEEEEEHHCCCCCCCCCC
QDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELIKPKDALYCVEGMIKFFMDYGNY
CCCCEEEEECCCCCEEEEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCC
ENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGSLELNIEEIDYSKPGVKIDIQDSRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCCEEEEECCCCC
IPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDNVENPMIRLGMTEGLYILNLNGNEAKR
CCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHH
ILEISKTISCNSQLEQSISCIGVPICQMGIQNSQKMLHEIIDYFRLQNNEEILNKAPKLY
HHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCEE
ISGCLNSCGVHQVGSIGLCGKKKNVDGISTDAFELFVGGSFEIGKTRLGKSLGDFKASDI
EEHHHCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCH
PEMLYKISDASSGNFYEWVNSNDNILNKITDKYKI
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA