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The map label for this gene is thiH [H]

Identifier: 126699772

GI number: 126699772

Start: 2494571

End: 2495947

Strand: Direct

Name: thiH [H]

Synonym: CD2156

Alternate gene names: 126699772

Gene position: 2494571-2495947 (Clockwise)

Preceding gene: 126699771

Following gene: 126699773

Centisome position: 58.15

GC content: 31.3

Gene sequence:

>1377_bases
ATGTTCATAAACCATGAACTTATTAATTCTTTATTAGAAGATGCAAAAAATTCAACTTCTGATGATATTGAAAAGGTACT
TGATAAGGCTGATAGAAGGGAAAAACTTAGTTATAAAGATATAGCTACACTTCTTGAAGTAGAAGATAAAAAACAACTAG
ATAGATTATTTAGTATAGCTGGGCAAATAAAAAATGAAATATATGGTAATAGAGTGGTTTTATTTGCCCCTCTTTATGTA
TCAAACTACTGTGTAAATGAATGTGTTTATTGCGGTTTTAGTAAATGCAACAAATTTAAAAGAAAAAAACTTACCATGGA
AGAGATAAAGGAAGAGGTTAAAATATTAGAAAAAATGGGTCATAAAAGACTTGCTCTAGAAGCTGGTGAAGACCCTAAAA
ATTGTGATATAAATTACATCTTAGATTGCTTAGATGCAATTTATTCAACATATAATGAAAACGGAAATATAAGAAGGGTA
AACGTTAACATAGCAGCTACAAGTGTAGATGAGTATAAATTACTAAAAGAAAAAGGAATAGGAACTTATATATTATTCCA
AGAAACTTATCACAAACCAACATTTATAAAAATGCATGGTCAATCTATAAAAAATGACTATTATTACCATTTAACAGCTT
TTGATAGAGCTATGGAAGCTGGTGTTGATGATGTTGGTGCAGGAGTTTTATTTGGTTTATCTGACCCTAAATTTGAAGTT
CTAGGACTTATGATGCACAACGAACACTTAGAGGAAAAATTTGGTGTAGGTTTCCATACTATATCTTTCCCTAGATTGAA
AAAAGCTGAAGGTATGAGCTTAGAAGATTTTCCTCACTTAGTTTCTGACGATATGTTCAAAAAAATAGTAGCTATAACTA
GATTGGCTGTTCCTTTTACTGGAATCATAATGTCCACTAGAGAAACTGCTGAAATGAGAAATGAGTTACTAAAATATGGT
GTATCACAAATTAGTGCAGGTTCTCTAACAGGTGTTGGAGGATATAAAGCATATGAAGATGGTGACAATACAGAACAATT
CGAAGTTGGAGACCATAGAAGCCCTGTTGAGGTGCTTAAAGAACTGATTACTGATGGATATATACCTAGTTATTGTACAG
CTTGTTATCGTAAAGGTAGAACTGGCGATAGATTCATGAGCTTAGCTAAAAGTCGACAAATCCATAATGTATGTACACCA
AATGCTCTTACTACATTAAATGAATTTTTAATCGATTATGGAGATGAAGAACTTAAAATTATGGGTAAAAAACTTATCGC
AGAAGAAATAGGTAAGATAGAAAGAGAAGATATAAGAAACATAGTATCTAACAATATGACTGCCTTAGAGCGTGGAGAAA
GAGATTTATACCTATAA

Upstream 100 bases:

>100_bases
AGCTTGTGAATATCACCAAAGTTTAGAAGAAAACATCAAGTCATTAGGACTTGAAGTTGACTATTCTCGCGGTGATAATA
TTGAATGGAGGCGTTTGTAA

Downstream 100 bases:

>100_bases
AATTTTATTACTTATATATTATTTATTGTATATTCCATAGGTTAGTAGTAATATTATCTAAAGGGAAAGTTTAAGGGAGG
GAGATTAACATTGAAAGATA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATLLEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYV
SNYCVNECVYCGFSKCNKFKRKKLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAMEAGVDDVGAGVLFGLSDPKFEV
LGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLEDFPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYG
VSQISAGSLTGVGGYKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSRQIHNVCTP
NALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALERGERDLYL

Sequences:

>Translated_458_residues
MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATLLEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYV
SNYCVNECVYCGFSKCNKFKRKKLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAMEAGVDDVGAGVLFGLSDPKFEV
LGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLEDFPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYG
VSQISAGSLTGVGGYKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSRQIHNVCTP
NALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALERGERDLYL
>Mature_458_residues
MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATLLEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYV
SNYCVNECVYCGFSKCNKFKRKKLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAMEAGVDDVGAGVLFGLSDPKFEV
LGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLEDFPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYG
VSQISAGSLTGVGGYKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSRQIHNVCTP
NALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALERGERDLYL

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=369, Percent_Identity=30.0813008130081, Blast_Score=179, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52173; Mature: 52173

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATLLEVEDKKQLDRLFSIA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRKKLTMEEIKEEVKILEKMG
HHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
HKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRVNVNIAATSVDEYKLLKEKGI
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCCC
GTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAMEAGVDDVGAGVLFGLSDPKFEV
CEEEEEECCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEECCCCCCHHH
LGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLEDFPHLVSDDMFKKIVAITRLAVPFT
HHHHHHHHHHHHHHCCCEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIIMSTRETAEMRNELLKYGVSQISAGSLTGVGGYKAYEDGDNTEQFEVGDHRSPVEVLK
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHH
ELITDGYIPSYCTACYRKGRTGDRFMSLAKSRQIHNVCTPNALTTLNEFLIDYGDEELKI
HHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
MGKKLIAEEIGKIEREDIRNIVSNNMTALERGERDLYL
HHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCCCCCC
>Mature Secondary Structure
MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATLLEVEDKKQLDRLFSIA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRKKLTMEEIKEEVKILEKMG
HHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
HKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRVNVNIAATSVDEYKLLKEKGI
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCCC
GTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAMEAGVDDVGAGVLFGLSDPKFEV
CEEEEEECCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEECCCCCCHHH
LGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLEDFPHLVSDDMFKKIVAITRLAVPFT
HHHHHHHHHHHHHHCCCEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIIMSTRETAEMRNELLKYGVSQISAGSLTGVGGYKAYEDGDNTEQFEVGDHRSPVEVLK
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHH
ELITDGYIPSYCTACYRKGRTGDRFMSLAKSRQIHNVCTPNALTTLNEFLIDYGDEELKI
HHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
MGKKLIAEEIGKIEREDIRNIVSNNMTALERGERDLYL
HHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]