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The map label for this gene is adhE [H]

Identifier: 126699527

GI number: 126699527

Start: 2218012

End: 2219481

Strand: Direct

Name: adhE [H]

Synonym: CD1917

Alternate gene names: 126699527

Gene position: 2218012-2219481 (Clockwise)

Preceding gene: 126699526

Following gene: 126699528

Centisome position: 51.7

GC content: 31.7

Gene sequence:

>1470_bases
ATGACACTTTTAGATAAAGATTTAGTATCTATACAAGAAGTAAGAACTCTTGTAAGAAGATCTAAAGTGGCTCAAGAGAA
ACTTTCTAAAATGAGTCAAGAGCAAATAGATAAAATAGTAAAAGCTGTTGTAGATGCAGCATTTAATAATGCTACTAAAT
TAGCTAAAATGGCAAATGAAGAAACTGGATTTGGAAAATGGGAAGATAAAGTAATTAAAAATACTTTTGCATCTATGGGA
GTTTATGAAACTTTAAAGAACACAAAAACTATTGGAATAATTGGTGAAGATAAGGAAGCCAATACGATAGATATAGCTAC
ACCAGTTGGAGTTGTGGCAGGTCTTATACCTTCAACAAACCCAACATCAACAGTAATATACAAAACTATAATATCTCTAA
AAGCAGGAAATACTATAGTTTTCTCTCCACATCCAAATGCAAAAAACTGCATACTTGAAACAGTTAATATAATAAGTGAT
GCTGCTATAAAGGCTGGAGCTCCAGAGGATTCAATATTATGTATAACTAATCCAAGTATAGTTGGAACCAATGAATTGAT
GAAACATAAAGATACAAACTTAATACTAGCAACTGGAGGAGAAGCAATGGTTAGAGCTGCTTACTCTTCAGGAACACCAG
CTATTGGAGTTGGACCAGGAAATGGACCAGCATTTATAGATAAATCAGCAAATGTTAAATTAGCAATCAAAAGAATAATA
GATTCTAAAACTTTTGATAATGGTGTTATATGTGCATCAGAACAATCTATAGTAATAGAAAAATCTATGGAAGAAACTGT
TTTAAAAGAGCTTAAAAATCAAGGTGCATATGTTTTAAATGAAGAGGAAAGTGAAAGATTAGCAAAATTCATATTAAGAG
CTAATGGTACTATGAATCCACAAATAGTTGGTAAAAGTGTTGAGGTAATAGCAAATTTAGCAGACTTAACAAATGTACCT
AAAAATACAAAAGTTCTAGTTGCAAGAGAAAATAAAGTTGGTCACAATGTAGCCTATTCAAGAGAAAAACTTACACCAAT
ACTAGCGTTCTATGTTGAAGAAAATGTAGAAGCTATTATGAACAGATGCAGAGAAATATTGTTAAATGAAGGTGCAGGTC
ATACATTCTCAATGCATGTAAATGATGAAGAATTGGTAAAAAGATTTGCCCTTAATATGCCAGTATCAAGAATACTTGTG
AATACTCCTTCAGCACTTGGAGGAATAGGTGCATCAACAAACCTTGTACCAGCACTAACACTTGGTTGTGGTGCAATAGG
TGGAAGTTCAACTTCAAACAATATAGGACCACTTGATTTAATAAATGTTAAAAGAGTAGCTTATGGAATCAAAGAAATAG
AAGAACTTGGAAGAGATAAATCTCAAGTTGAAAAATCAATGTTTGACTCAATGGATAAAGAAGAATTAATAAATCTTCTT
GTTCAAAAACTATTCAAAGAACTAGCATAA

Upstream 100 bases:

>100_bases
AGAAATTAGATTCTTCAATAACTTATAAAGATTTAAATGTTCTTAAAAAAGAAGAATTAATAAACTTAGTTAAAAAGCTT
AGTAGAAGGGATAGATAGTT

Downstream 100 bases:

>100_bases
CTAAAATAAACAATGAAACTTATGGCTATCGCTATAAATTTCTAAAAATATAAACTATAAAATTAAATTTTTGGAGGATA
AGATTATGGCAAGTGCAAAC

Product: ethanolamine/propanediol utilisation aldehyde-alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVVDAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMG
VYETLKNTKTIGIIGEDKEANTIDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVGPGNGPAFIDKSANVKLAIKRII
DSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGAYVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVP
KNTKVLVARENKVGHNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALNMPVSRILV
NTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGIKEIEELGRDKSQVEKSMFDSMDKEELINLL
VQKLFKELA

Sequences:

>Translated_489_residues
MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVVDAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMG
VYETLKNTKTIGIIGEDKEANTIDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVGPGNGPAFIDKSANVKLAIKRII
DSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGAYVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVP
KNTKVLVARENKVGHNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALNMPVSRILV
NTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGIKEIEELGRDKSQVEKSMFDSMDKEELINLL
VQKLFKELA
>Mature_488_residues
TLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVVDAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGV
YETLKNTKTIGIIGEDKEANTIDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISDA
AIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVGPGNGPAFIDKSANVKLAIKRIID
SKTFDNGVICASEQSIVIEKSMEETVLKELKNQGAYVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPK
NTKVLVARENKVGHNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALNMPVSRILVN
TPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGIKEIEELGRDKSQVEKSMFDSMDKEELINLLV
QKLFKELA

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=445, Percent_Identity=46.5168539325843, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788797, Length=381, Percent_Identity=32.2834645669291, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 52780; Mature: 52648

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVVDAAFNNATKLAKMANE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC
ETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANTIDIATPVGVVAGLIPSTN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCHHHHHHHCCCCCC
PTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISDAAIKAGAPEDSILCITNPSI
CCHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
VGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVGPGNGPAFIDKSANVKLAIKRII
CCCHHHHHCCCCCEEEEECCHHHHEEHHCCCCCEEEECCCCCCEEEECCCCHHHHHHHHH
DSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGAYVLNEEESERLAKFILRANGTMNP
CCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCH
QIVGKSVEVIANLADLTNVPKNTKVLVARENKVGHNVAYSREKLTPILAFYVEENVEAIM
HHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRCREILLNEGAGHTFSMHVNDEELVKRFALNMPVSRILVNTPSALGGIGASTNLVPALT
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHEEECCHHHCCCCCCCCCHHHHH
LGCGAIGGSSTSNNIGPLDLINVKRVAYGIKEIEELGRDKSQVEKSMFDSMDKEELINLL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
VQKLFKELA
HHHHHHHCC
>Mature Secondary Structure 
TLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVVDAAFNNATKLAKMANE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC
ETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANTIDIATPVGVVAGLIPSTN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCHHHHHHHCCCCCC
PTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISDAAIKAGAPEDSILCITNPSI
CCHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
VGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVGPGNGPAFIDKSANVKLAIKRII
CCCHHHHHCCCCCEEEEECCHHHHEEHHCCCCCEEEECCCCCCEEEECCCCHHHHHHHHH
DSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGAYVLNEEESERLAKFILRANGTMNP
CCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCH
QIVGKSVEVIANLADLTNVPKNTKVLVARENKVGHNVAYSREKLTPILAFYVEENVEAIM
HHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRCREILLNEGAGHTFSMHVNDEELVKRFALNMPVSRILVNTPSALGGIGASTNLVPALT
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHEEECCHHHCCCCCCCCCHHHHH
LGCGAIGGSSTSNNIGPLDLINVKRVAYGIKEIEELGRDKSQVEKSMFDSMDKEELINLL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
VQKLFKELA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]