Click here to switch to the map view.

The map label for this gene is ispH

Identifier: 126699426

GI number: 126699426

Start: 2100159

End: 2100998

Strand: Direct

Name: ispH

Synonym: CD1818

Alternate gene names: 126699426

Gene position: 2100159-2100998 (Clockwise)

Preceding gene: 126699425

Following gene: 126699427

Centisome position: 48.95

GC content: 28.93

Gene sequence:

>840_bases
ATGAACATAAAAATAGCAAAAAATGCTGGCTTTTGTTTTGGAGTAAAAAGAGCAATGAAAATGGCATGGGATGAAGTTGA
AAAAAATGATTCTGGCATATATGCTTTAGGTCCTCTTATTCATAACAAGCAAGCAGTAGCAAAATATGAAGAAAAAGGTC
TTAAGACTGTGAATGAAATTGATACAATACCAAATCATGAAAATATGATAATAAGGTCTCATGGAGTTCCTGAAAATATA
TATAAGGAAGCCAAAGATAAAAAATTAAAAATTGTGGATACTACATGTCCATTTGTAAAGAAAATACATACAGTAGTAAG
TGAATATCATAATAAAGGCTATGAAATAATAGTTATTGGAGATATGAAGCATCCAGAAGTTATAGGTATAAATGGATGGT
GTGAAAATTCAGCTATAATAATAAAGACCTTAGAACAAATGGAAAATATGGAATTTGATAACTCCAAAAGATATTGTCTT
GTTGCACAGACTACAATAAATCCAGAGTTGTATATAAGTATAGTTAATAAACTATCAGATAAATTAGAAGAAATAGTATT
TAATGATACAATTTGTTCAGCTACAAAAACAAGACAAGAATCAGCTAAAGAGTTAGCTAAAGAAGTTGATTGTATGATAG
TTATAGGAGGTAAACACAGTTCAAATACTCAAAAGTTAGTAAAGGTTTGTGAAGACTTAGTACCAACTTTTGCAATAGAG
ACTAAAGATGAATTGGATGTAAATACGCTTAAAAAATATAAAAATTTGGGTATTACAGCAGGGGCATCTACACCTAATTG
GATAATAGAAGAAGTTGTAACATTCCTTGAAAATTTATAA

Upstream 100 bases:

>100_bases
GGAAAAATTGAGTAATGATGACCATGAAAGGATATCACAAGAGATTTTAGAAGTGATAAAACAAAATTACTTTAAATATT
CAAAATAAGGGGGTAAAGTT

Downstream 100 bases:

>100_bases
TTTGATAGGTAATAAAAATAGGTGGACATTGTTATAATTTATCATAATATCCACCTTTGATTTCATATTACATGTGGTAA
ATAGATAACCTAATATCAAA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQAVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENI
YKEAKDKKLKIVDTTCPFVKKIHTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGKHSSNTQKLVKVCEDLVPTFAIE
TKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFLENL

Sequences:

>Translated_279_residues
MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQAVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENI
YKEAKDKKLKIVDTTCPFVKKIHTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGKHSSNTQKLVKVCEDLVPTFAIE
TKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFLENL
>Mature_279_residues
MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQAVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENI
YKEAKDKKLKIVDTTCPFVKKIHTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGKHSSNTQKLVKVCEDLVPTFAIE
TKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFLENL

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=285, Percent_Identity=30.1754385964912, Blast_Score=157, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CLOD6 (Q187C6)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001088323.1
- STRING:   Q187C6
- GeneID:   4914744
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1818
- NMPDR:   fig|1496.1.peg.2987
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK12360
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 31467; Mature: 31467

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQAVAKYEEKGLKTVNEI
CCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCHHHHHHHHHHCCHHHHHH
DTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKIHTVVSEYHNKGYEIIVIG
HCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEE
DMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCLVAQTTINPELYISIVNKLSD
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
KLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGKHSSNTQKLVKVCEDLVPTFAIE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEC
TKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFLENL
CCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQAVAKYEEKGLKTVNEI
CCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCHHHHHHHHHHCCHHHHHH
DTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKIHTVVSEYHNKGYEIIVIG
HCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEE
DMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCLVAQTTINPELYISIVNKLSD
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
KLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGKHSSNTQKLVKVCEDLVPTFAIE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEC
TKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFLENL
CCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA