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The map label for this gene is ykfA [H]

Identifier: 126699001

GI number: 126699001

Start: 1619218

End: 1620081

Strand: Direct

Name: ykfA [H]

Synonym: CD1399

Alternate gene names: 126699001

Gene position: 1619218-1620081 (Clockwise)

Preceding gene: 126698996

Following gene: 126699002

Centisome position: 37.74

GC content: 27.66

Gene sequence:

>864_bases
ATGTTAGGAATAATTGCGCCATCTGGGCCATTAAGAAATACAAGTTTGGAGGAAATAAAGTTTAATTTAGAAAGTTATGG
TTACGAAGTTAAGTTTTCTGAAAGTTGTAGTTTAAATTATAAAGGTTATTTAGCTGGTAATGATGATATTAGAGCAAGGG
ATATAGAAGATATGTTTTTAGATAAAGATGTAGATATAATTATGTGCTTAAGAGGAGGTTATGGAACAACAAGAATATTG
GATAAAATAAATTATGATATAATAAAGCAAAATCCAAAACCATTTATAGGTTTTTCTGATATAACAGGTTTAAATTTAGC
TTTCTATAAAAATTGTGGTCTTTTACCATATCATGGAATAATGGCAGCAAATGTAGGTAAATGGGATAAATTTACCTATA
AATCATTGGTAAATGCTTTAGAATTTAAAGATGAATTATATTTAGAAAATCCAAAAGAAGAAAAGATTTATACTGTATGT
GAAGGTAAAGCAGAGGGAATAATAATGGGAGGAAATTTATCCTTAATAATTGCCACAATGGGAACAAAATATGAAATAGA
TGCAAAAGATAAAATTTTGTTTATTGAGGAAATTGGAGAACCTCAATATAAACTGGATAGAATGTTAACACAACTATATT
CTTCAGGAAAACTTGAGGAATGTAATGGTATAATTTTTGGAGATTTTAAGGATTGTATTGAAGAAAATGATTTGATGGAA
TTACTAATAGAATTTGCAAATAAGGTCAATAAACCTTCTATATATAATCTTCAGTCAGGACATTGTATCCCAATGATAAC
TATTCCTTTGGGCAGAATGTGCGAATTAGATGCTACAGAAAAAATCGTTAAATTAAAAAAATAG

Upstream 100 bases:

>100_bases
ATCATTATTATGTATCAAGTACAAATAAAATATCTTATAATTATATTAGAGATATTTAGTATGGAGAAGAATATATCATT
TTATAAGAAGAGGTGTTTAA

Downstream 100 bases:

>100_bases
TGTGTATATAAATATAATAGAAATATGGTATAATCTTATACAAGTAAAAATTTAGCTAGGAGGATGAAAAATGTCTGATG
ATAGAATAATAGACTTTAAC

Product: muramoyltetrapeptide carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYLAGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRIL
DKINYDIIKQNPKPFIGFSDITGLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKLEECNGIIFGDFKDCIEENDLME
LLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCELDATEKIVKLKK

Sequences:

>Translated_287_residues
MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYLAGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRIL
DKINYDIIKQNPKPFIGFSDITGLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKLEECNGIIFGDFKDCIEENDLME
LLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCELDATEKIVKLKK
>Mature_287_residues
MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYLAGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRIL
DKINYDIIKQNPKPFIGFSDITGLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKLEECNGIIFGDFKDCIEENDLME
LLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCELDATEKIVKLKK

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=303, Percent_Identity=27.7227722772277, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 32511; Mature: 32511

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYLAGNDDIRARDIEDMFL
CEEEECCCCCCCCCCHHHHEEEHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHC
DKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDITGLNLAFYKNCGLLPYHGI
CCCCCEEEEEECCCCHHHHHHHCCHHEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCE
MAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVCEGKAEGIIMGGNLSLIIATM
EECCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCEEEEEECCCCCEEEECCCEEEEEEEC
GTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKLEECNGIIFGDFKDCIEENDLME
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCHHHH
LLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCELDATEKIVKLKK
HHHHHHHHCCCCCEEEECCCCEEEEEEECCCCEECCCCHHHHHCCCC
>Mature Secondary Structure
MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYLAGNDDIRARDIEDMFL
CEEEECCCCCCCCCCHHHHEEEHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHC
DKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDITGLNLAFYKNCGLLPYHGI
CCCCCEEEEEECCCCHHHHHHHCCHHEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCE
MAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVCEGKAEGIIMGGNLSLIIATM
EECCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCEEEEEECCCCCEEEECCCEEEEEEEC
GTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKLEECNGIIFGDFKDCIEENDLME
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCHHHH
LLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCELDATEKIVKLKK
HHHHHHHHCCCCCEEEECCCCEEEEEEECCCCEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]