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The map label for this gene is rob [H]

Identifier: 126698030

GI number: 126698030

Start: 538777

End: 539613

Strand: Direct

Name: rob [H]

Synonym: CD0454

Alternate gene names: 126698030

Gene position: 538777-539613 (Clockwise)

Preceding gene: 126698029

Following gene: 126698031

Centisome position: 12.56

GC content: 29.99

Gene sequence:

>837_bases
ATGTCTTACAAAGTAATTAATGTCATAACTGCTATTGACTATATAGAGGAGCATTTATCAGAAAAGCTGGATTTAGATAT
AGTTGCTAATGCTGTTCATTACTCAAAATATCACCTGCATAGAACTTTCACAACTTCTGTTGGTCTAACAATGCATGATT
ACATAAAGCGGAGAAAGTTGACAGAAGCAGCAAAATTATTGGTATTTTCAAAGAAACCAATTATTGAAATTGCATTGATA
GCTGGATATGAAAGTCAACAGGCATTCACTTCTATATTTAAAGCTATGTATAAAAAATCTCCAAATAAATATAGGAAAGA
ACAAGAATTTTATCCTTTGCAGCTAAGATTTGTTTTGAAAAAAGATAACCTATTTTTAAAGAATGTACTAGAGTTGGAAA
AAGAAATTAAACTTGCAAGTATGTCTGACATACCATTATGGATGAATTTAGTGAGATTAGTCATAGATGGATTTCCCAAT
TTGCAAGAGGAAGAATATATATATCAATTAGAACAGTATATTTTAGAAGAAAGAGCTTTAATATTAAAACTTAATAATGT
CGCTATTGCAAATATGGTGTTTAATAGAGAAACTAGAAGTATTGATTTTTTTGGAATACATCCACAGTATCGTAATTCAG
ACATTGCACAAGTGTTTTTGAAGAAAGTTATTGAAGATTTCTTGATAGATACAGATATTTCAATTACTACCTTTCGAGAG
GGTGACAAAGCTGATACAGGACATAGAGATATGATAAAGAAGTTGGGCTTTGCAGAAGCAGAATTGCTTGTGGAATTTGG
ATATCCTACTCAAAAATTTATTCTTCCTTTAAGTTAA

Upstream 100 bases:

>100_bases
TGGTCTGCGTGTAGTTCCTTGTAAACTGACCTAAGATTCATTAAATATAACTAAGTTATAATTAATATAATGTTGCATGG
ATAAAAGGAGGGAAGCTGCA

Downstream 100 bases:

>100_bases
ATAGCAAGAATGATTAAAAACTCCAAAACACAATTAAGTATAATGATTCAGTGTAAGTACTTAATTAACAAAGGAGGATT
TAAATGTCTGAAAATAAACA

Product: AraC family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFTTSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALI
AGYESQQAFTSIFKAMYKKSPNKYRKEQEFYPLQLRFVLKKDNLFLKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQVFLKKVIEDFLIDTDISITTFRE
GDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILPLS

Sequences:

>Translated_278_residues
MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFTTSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALI
AGYESQQAFTSIFKAMYKKSPNKYRKEQEFYPLQLRFVLKKDNLFLKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQVFLKKVIEDFLIDTDISITTFRE
GDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILPLS
>Mature_277_residues
SYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFTTSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIA
GYESQQAFTSIFKAMYKKSPNKYRKEQEFYPLQLRFVLKKDNLFLKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPNL
QEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQVFLKKVIEDFLIDTDISITTFREG
DKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILPLS

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=133, Percent_Identity=33.8345864661654, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790497, Length=94, Percent_Identity=40.4255319148936, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87081928, Length=101, Percent_Identity=39.6039603960396, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789993, Length=98, Percent_Identity=38.7755102040816, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32546; Mature: 32415

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFTTSVGLTMHDYIKRRKL
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEHHHCCCHHHHHHHHHHH
TEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPNKYRKEQEFYPLQLRFVLK
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEE
KDNLFLKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPNLQEEEYIYQLEQYILEERAL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCE
ILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQVFLKKVIEDFLIDTDISITTFRE
EEEECCEEEEHHEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
GDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILPLS
CCCCCCCHHHHHHHCCCHHHHHEEECCCCCHHEEEECC
>Mature Secondary Structure 
SYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFTTSVGLTMHDYIKRRKL
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEHHHCCCHHHHHHHHHHH
TEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPNKYRKEQEFYPLQLRFVLK
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEE
KDNLFLKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPNLQEEEYIYQLEQYILEERAL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCE
ILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQVFLKKVIEDFLIDTDISITTFRE
EEEECCEEEEHHEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
GDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILPLS
CCCCCCCHHHHHHHCCCHHHHHEEECCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]