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The map label for this gene is rocR [H]

Identifier: 126698015

GI number: 126698015

Start: 524357

End: 525757

Strand: Direct

Name: rocR [H]

Synonym: CD0441

Alternate gene names: 126698015

Gene position: 524357-525757 (Clockwise)

Preceding gene: 126698014

Following gene: 126698016

Centisome position: 12.22

GC content: 28.41

Gene sequence:

>1401_bases
TTGAACCAAGAGATATTTTTTAAAATAATGAATGAAATATTGGAAAATATTGATGAAGGGGTACATTTTGTTAATAAAGA
CGGGAAAACTATAATCTACAATAATTCTGTACAAAAAATGGAAAAAATGAACAGACACGATATTTTAACCAATTCATTTT
TTGATATAGTCAACAAAATGAAAATAAACAGGAGTACACTATTAGAAGTTTTGGAAAAAAGGGAAGTTATCAAAGACAAT
ATTCAAAAATATCTGGATAAAAATGGAAAAGAGATAACAGCAATTAATACTACAATACCAATTGATATTAAAGGCGAGTT
TGTAGGGGCACTTGAAATATCAAAGAATATGACAACGATAGAAAATTTACTAAACAGAAATTATAAAAAAAATTTAGTCA
ATAAAGTTAGTAGTAGACAAAAAAAGGGTTATGAATTTGATGATATTTTAGGGGAAAGTAATTTGATTAAAGAAGCAATA
GCTAAATCTAAGAAAGCTTGCAAGTCTGATGCTTCAGTATTTATATACGGTGAAACTGGAACGGGTAAAGAGTTATTTAG
CCAGTCCATTCACTATGGAAGTAATAGGAGAAACTATCCATTTATAGCACAAAATTGTGCAGCTTTGCCGGAATCACTAT
TCGAAGGTATCCTTTTTGGAACTAGCAAGGGAGGATTTACAGGAGCAATAGATAGGCCAGGTTTATTTGAACAAGCTAAC
AAAGGGACATTGTTATTGGATGAGATAAATTCCATGCCTATTATGTTACAGGCTAAACTATTAAGGGTTTTACAAGAAGG
TTATGTAAGAAGGGTTGGAGGAACTAAGGATATACCTATAGATGTAAGAATTATTGCAACGACAAATGAAAGTCCAAAGG
TAATTTTAAACGAGCATAAAATGCGAAAAGATTTATATTATAGGCTTAACATAATTCATATAAAAATTCCTCCTCTTAGA
GATAGAAAAGAGGACATACCTCTATTGTGCAAAAAATTTATTTCAAAGTATAATAAAAAGTTAAATAAAAAAGTGGTAGG
AATTTCAGATGAAGCTATTAAAATTTTAAAAAATTACGATTGGCAAGGTAACATAAGAGAGCTAGAAAACATAATTTATT
GTACTATGAGTATGATGGAAGATGAGGTACAAATAGAAACAAGTATGATTAATCTAAACGACTATTACAATCTTAAAGAG
AATAAAGAGAGTAACAATTATTCTTTAAACGATTTGGAGGACAAAACACTTAGTAAGATTATCAGTGAGATAGAAGAAAA
GTATATATATGAATCATTAGAAAAGGCATCTTACAATATAACTAAAGCTGCATCAATTTTAGGTATGAATAGGCAAAATT
TACAGTATAAAATAAAAAAATATAATATCAAAATATTATAA

Upstream 100 bases:

>100_bases
GTACATAAAAAATATCATAAATATATGATAAGATAAATACAATAGCAACATTTTATTAATTTTTTGGGTTTTTATATATT
TAGGTACTGGGGTGATATAG

Downstream 100 bases:

>100_bases
ATAAGCAAGATAAAAAAACAAAATCAAAGTAAAATAAAAAAGCAAAATAGAGGAGCAAAATTATTATAAAAGAGCAAAAT
ATTTTGCTCTTTTTTTTATT

Product: arginine utilization sigma-54 dependent regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSFFDIVNKMKINRSTLLEVLEKREVIKDN
IQKYLDKNGKEITAINTTIPIDIKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAI
AKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFTGAIDRPGLFEQAN
KGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLR
DRKEDIPLLCKKFISKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKE
NKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIKKYNIKIL

Sequences:

>Translated_466_residues
MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSFFDIVNKMKINRSTLLEVLEKREVIKDN
IQKYLDKNGKEITAINTTIPIDIKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAI
AKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFTGAIDRPGLFEQAN
KGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLR
DRKEDIPLLCKKFISKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKE
NKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIKKYNIKIL
>Mature_466_residues
MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSFFDIVNKMKINRSTLLEVLEKREVIKDN
IQKYLDKNGKEITAINTTIPIDIKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAI
AKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFTGAIDRPGLFEQAN
KGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLR
DRKEDIPLLCKKFISKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKE
NKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIKKYNIKIL

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=329, Percent_Identity=42.2492401215805, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI87082117, Length=374, Percent_Identity=36.8983957219251, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1790437, Length=394, Percent_Identity=35.2791878172589, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=37.5384615384615, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789087, Length=229, Percent_Identity=47.5982532751092, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788905, Length=311, Percent_Identity=39.871382636656, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=36.6366366366366, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=35.5828220858896, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786524, Length=243, Percent_Identity=41.1522633744856, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082152, Length=305, Percent_Identity=36.0655737704918, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1787583, Length=319, Percent_Identity=32.6018808777429, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081858, Length=463, Percent_Identity=26.3498920086393, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=31.9502074688797, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53759; Mature: 53759

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSFFDIVNKM
CCHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGALEISKNMTTI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCEEEEEEECCCHHHH
ENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKSDASVFIYGETG
HHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFTGAIDRPGLFEQAN
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHCCC
KGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRIIATTNESPKVILNEHK
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEECHHH
MRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNKKVVGISDEAIKILKNYD
HHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHCCC
WQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKENKESNNYSLNDLEDKTLSKI
CCCCHHHHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIKKYNIKIL
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSFFDIVNKM
CCHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGALEISKNMTTI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCEEEEEEECCCHHHH
ENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKSDASVFIYGETG
HHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFTGAIDRPGLFEQAN
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHCCC
KGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRIIATTNESPKVILNEHK
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEECHHH
MRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNKKVVGISDEAIKILKNYD
HHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHCCC
WQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKENKESNNYSLNDLEDKTLSKI
CCCCHHHHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIKKYNIKIL
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]