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The map label for this gene is manZ [H]

Identifier: 126697861

GI number: 126697861

Start: 349331

End: 350194

Strand: Direct

Name: manZ [H]

Synonym: CD0289

Alternate gene names: 126697861

Gene position: 349331-350194 (Clockwise)

Preceding gene: 126697860

Following gene: 126697862

Centisome position: 8.14

GC content: 32.75

Gene sequence:

>864_bases
ATGCAAGAAATAAAAAATAAAGAAGTTAAAAATGGACAAGGTCCAACTGAAGTTAATAAGACAAGAGTACTTACAAAGAA
AGATGTAACAAAAACATATCTAAGATGGTGGTGGACAGCAGAATTATCCAACTCTTTTGAAAGAATGCAAGCATTAGCAG
TTTGTGCAAGTTTCACTCCGGCTTTAGAAAAGTTATATAAGAGAAAAGAAGATTTAGTAGATGCGTTAAAGAGACACTTA
CAATTTTTTAATACTCAAGCTATATGGGGAGGATTAATTCACGGTACAGTTTTAGCTATGGAAGAAGAGAAAGCTACTGA
AGGAAAAATCCCAGGAGAAGTTATTTCAGGTGTTAAGAATGGTCTTATGGGACCTTTGGCTGGAATTGGAGATACTTTAG
ATTTTGGTACATTTCAAACAATATTCTTAGCTTTAGGTGCTTCATTTGGAGCAGAAGGAAGTGTTATAGGTGCATTTTTC
CCAATAATGTTTAGTATATTACTATTTTGCGAAGGTTATTACTTATTTCATTTAGGATATTCTTTAGGTAGAGATTCAAT
TAAGAAAATTCTATCAGGTGGTATTGTCAATAAGATAATTGATGGTGCTTCAATTCTAGGAATGTTTATGATGGGAGCAT
TATCTGCAACTACAGTTAAGCTTTCAACTCCGCTATCATTTGATATTGGTGGTAAAGCAATTGTTGTTCAAGATACATTA
AATATGATTGCTCCAGGTTTATTACCACTTGGTGTTGTATTCTTCGTATATTGGGGAATGAAATACAAGAAGTGGACAAT
AACTAAGTTATTAGTAATTTTGGTTGTATTAGCATTGGTAGGGTCATTCATTGGAATATTCTAA

Upstream 100 bases:

>100_bases
AATATTTTGAAATATCAATTACTGCAGCTGCAATATTTGGTGTTTGTATAGCTCTTTTAATTACCTTTATGAAAGAAGAC
AAAAGGGTAGGTGAAGTATA

Downstream 100 bases:

>100_bases
TTCACAGTGATTAAAATATAGAATTTAATTTTAGGAGGAGAAAGAAATGTCAGAAAAAATGTATACAGAAAAGGAATATT
TAGAAGGAATTAGAGGAGCA

Product: PTS system transporter subunit IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSNSFERMQALAVCASFTPALEKLYKRKEDLVDALKRHL
QFFNTQAIWGGLIHGTVLAMEEEKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATTVKLSTPLSFDIGGKAIVVQDTL
NMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVVLALVGSFIGIF

Sequences:

>Translated_287_residues
MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSNSFERMQALAVCASFTPALEKLYKRKEDLVDALKRHL
QFFNTQAIWGGLIHGTVLAMEEEKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATTVKLSTPLSFDIGGKAIVVQDTL
NMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVVLALVGSFIGIF
>Mature_287_residues
MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSNSFERMQALAVCASFTPALEKLYKRKEDLVDALKRHL
QFFNTQAIWGGLIHGTVLAMEEEKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATTVKLSTPLSFDIGGKAIVVQDTL
NMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVVLALVGSFIGIF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=272, Percent_Identity=39.7058823529412, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1789529, Length=268, Percent_Identity=31.7164179104478, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 31439; Mature: 31439

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSNSFERMQALAVCASFTP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEEEKATEGKIPGEVISGVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCCCHHHHHHHHH
GLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFFPIMFSILLFCEGYYLFHLGY
CCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
SLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATTVKLSTPLSFDIGGKAIVVQDTL
HHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEECCCCCEEEEEHHH
NMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVVLALVGSFIGIF
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSNSFERMQALAVCASFTP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEEEKATEGKIPGEVISGVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCCCHHHHHHHHH
GLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFFPIMFSILLFCEGYYLFHLGY
CCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
SLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATTVKLSTPLSFDIGGKAIVVQDTL
HHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEECCCCCEEEEEHHH
NMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVVLALVGSFIGIF
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]