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The map label for this gene is buk [H]

Identifier: 126697685

GI number: 126697685

Start: 157178

End: 158257

Strand: Direct

Name: buk [H]

Synonym: CD0113

Alternate gene names: 126697685

Gene position: 157178-158257 (Clockwise)

Preceding gene: 126697684

Following gene: 126697686

Centisome position: 3.66

GC content: 33.06

Gene sequence:

>1080_bases
ATGAGCAAAATATTTAAAATCTTAACAATAAATCCTGGTTCGACATCAACTAAAATAGCTGTATTTGATAATGAGGATTT
AGTATTTGAAAAAACTTTAAGACATTCTTCAGAAGAAATAGGAAAATATGAGAAGGTGTCTGACCAATTTGAATTTCGTA
AACAAGTAATAGAAGAAGCTCTAAAAGAAGGTGGAGTAAAAACATCTGAATTAGATGCTGTAGTAGGTAGAGGAGGACTT
CTTAAACCTATAAAAGGTGGTACTTATTCAGTAAGTGCTGCTATGATTGAAGATTTAAAAGTGGGAGTTTTAGGAGAACA
CGCTTCAAACCTAGGTGGAATAATAGCAAAACAAATAGGTGAAGAAGTAAATGTTCCTTCATACATAGTAGACCCTGTTG
TTGTAGATGAATTAGAAGATGTTGCTAGAATTTCTGGTATGCCTGAAATAAGTAGAGCAAGTGTAGTACATGCTTTAAAT
CAAAAGGCAATAGCAAGAAGATATGCTAGAGAAATAAACAAGAAATATGAAGATATAAATCTTATAGTTGCACATATGGG
TGGAGGAGTTTCTGTTGGAGCTCATAAAAATGGTAAAATAGTAGATGTTGCAAACGCATTAGATGGAGAAGGACCTTTCT
CTCCAGAAAGAAGTGGTGGACTACCAGTAGGTGCATTAGTAAAAATGTGCTTTAGTGGAAAATATACTCAAGATGAAATT
AAAAAGAAAATAAAAGGTAATGGCGGATTAGTTGCATACTTAAACACTAATGATGCTAGAGAAGTTGAAGAAAGAATTGA
AGCTGGTGATGAAAAAGCTAAATTAGTATATGAAGCTATGGCATATCAAATCTCTAAAGAAATAGGAGCTAGTGCTGCAG
TTCTTAAGGGAGATGTAAAAGCAATATTATTAACTGGTGGAATCGCATATTCAAAAATGTTTACAGAAATGATTGCAGAT
AGAGTTAAATTTATAGCAGATGTAAAAGTTTATCCAGGTGAAGATGAAATGATTGCATTAGCTCAAGGTGGACTTAGAGT
TTTAACTGGTGAAGAAGAGGCTCAAGTTTATGATAACTAA

Upstream 100 bases:

>100_bases
ATTAACTTCTAGAGCAGACAGTGAAGAAACTAAACTAAACTCAATAGCTTTAGGTGTTTTAATGGCAGCAAAGGCATAAT
TTTAAGGGGGAAATAGGCAA

Downstream 100 bases:

>100_bases
TGATAAAAGAATAAGAATCATAACTGGTCACTATGGAAGTGGTAAAAGTGAATTTGCGATGAACTATGTTGTTAAGTTAA
GAGATATGGTTTCTGGCAAA

Product: butyrate kinase

Products: NA

Alternate protein names: BK [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYEKVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGL
LKPIKGGTYSVSAAMIEDLKVGVLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERSGGLPVGALVKMCFSGKYTQDEI
KKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLVYEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIAD
RVKFIADVKVYPGEDEMIALAQGGLRVLTGEEEAQVYDN

Sequences:

>Translated_359_residues
MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYEKVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGL
LKPIKGGTYSVSAAMIEDLKVGVLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERSGGLPVGALVKMCFSGKYTQDEI
KKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLVYEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIAD
RVKFIADVKVYPGEDEMIALAQGGLRVLTGEEEAQVYDN
>Mature_358_residues
SKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYEKVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLL
KPIKGGTYSVSAAMIEDLKVGVLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALNQ
KAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERSGGLPVGALVKMCFSGKYTQDEIK
KKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLVYEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADR
VKFIADVKVYPGEDEMIALAQGGLRVLTGEEEAQVYDN

Specific function: Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate [H]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=154, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693184, Length=154, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38696; Mature: 38565

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYEKVSDQFEFRKQVIEEA
CCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVGVLGEHASNLGGIIAKQIG
HHHCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHCEECCHHHHHHHHHHHHHC
EEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALNQKAIARRYAREINKKYEDIN
CCCCCCCHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERSGGLPVGALVKMCFSGKYTQDEI
EEEEECCCCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
KKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLVYEAMAYQISKEIGASAAVLKGDVK
HHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCE
AILLTGGIAYSKMFTEMIADRVKFIADVKVYPGEDEMIALAQGGLRVLTGEEEAQVYDN
EEEEECCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCEEEEECCCHHHCCCC
>Mature Secondary Structure 
SKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYEKVSDQFEFRKQVIEEA
CCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVGVLGEHASNLGGIIAKQIG
HHHCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHCEECCHHHHHHHHHHHHHC
EEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALNQKAIARRYAREINKKYEDIN
CCCCCCCHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERSGGLPVGALVKMCFSGKYTQDEI
EEEEECCCCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
KKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLVYEAMAYQISKEIGASAAVLKGDVK
HHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCE
AILLTGGIAYSKMFTEMIADRVKFIADVKVYPGEDEMIALAQGGLRVLTGEEEAQVYDN
EEEEECCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCEEEEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]