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The map label for this gene is cwlD [H]

Identifier: 126697677

GI number: 126697677

Start: 123166

End: 123870

Strand: Direct

Name: cwlD [H]

Synonym: CD0106

Alternate gene names: 126697677

Gene position: 123166-123870 (Clockwise)

Preceding gene: 126697676

Following gene: 126697679

Centisome position: 2.87

GC content: 28.37

Gene sequence:

>705_bases
GTGAGAAAGTACATAAAACATATAATTTTTAGTTTTGCAATGATATGTCTAGTAGTAGTATCCATATTTGAAATTAAAAA
TATTTCTGAAGATGTTATCAAGTATATGCCAGTAACAAACAAAACTATAATTTTAGATGCTGGTCATGGAGGCATTGATC
CAGGTGCATTAAATAAGGATAAGAGTACATCTGAAAAGGATATTAACCTAGCAATAACACTTAAGCTTAGAGAGCTTATA
GAATCAAGTGGTGGCCTTGTAATATTAACTCGAGAAGATGACAGTAGCCTTTATAAAGAAGAAAATAATAAAACTACAAG
ACAAAAATATAATGAGAATTTGAAAAATAGAAAAGAAATAATATCTAATTCTAATGCCAATATGTTTGTCTCTATACATC
TTAATGCATTTGAACAATCAAAATACTATGGAGCTCAAACTTTTTATCCTAAAGACAAACAAGACAGTAAAGAATTATCG
AAGTGTATTCAAGAAGAACTTAAAAGAGTAGTAGATAAGACAAACAACAGAGAAGTCAAACCAAGGGATGATATATATCT
TTTGAAAGACAATAATATTCCATCAGTACTGATAGAATGTGGTTTTTTATCAAATGAAAAAGAGTGTAAACTCTTAACTG
ATGAAACATATCAAGAAAAAATAGCATGGGCAATCTACATAGGAATACAAAAATATTTAAGTTGA

Upstream 100 bases:

>100_bases
AATAAATAAAGATGTATCAATATTAAAAAAATTTAAATGTTTGTACTAATATATGGAATATTATAGTGTTTTTATAAGTA
TACATATACAGGGTGATGTT

Downstream 100 bases:

>100_bases
AATAACTATAAAAATAGGTCTAAATATGTAAATAGCTTGTATTAAAGCAGTTAAAATGCATTAATATAGGCTATTTTTAT
TTTGACAAAAAAATATTTAA

Product: germination-specific N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELI
ESSGGLVILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
KCIQEELKRVVDKTNNREVKPRDDIYLLKDNNIPSVLIECGFLSNEKECKLLTDETYQEKIAWAIYIGIQKYLS

Sequences:

>Translated_234_residues
MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELI
ESSGGLVILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
KCIQEELKRVVDKTNNREVKPRDDIYLLKDNNIPSVLIECGFLSNEKECKLLTDETYQEKIAWAIYIGIQKYLS
>Mature_234_residues
MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELI
ESSGGLVILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
KCIQEELKRVVDKTNNREVKPRDDIYLLKDNNIPSVLIECGFLSNEKECKLLTDETYQEKIAWAIYIGIQKYLS

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=236, Percent_Identity=26.6949152542373, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790611, Length=239, Percent_Identity=26.3598326359833, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26960; Mature: 26960

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTIILDAGHGGIDPGALNKD
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
KSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEENNKTTRQKYNENLKNRKEI
CCCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHH
ISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVK
HCCCCCEEEEEEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PRDDIYLLKDNNIPSVLIECGFLSNEKECKLLTDETYQEKIAWAIYIGIQKYLS
CCCCEEEEECCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHEEHHHHCC
>Mature Secondary Structure
MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTIILDAGHGGIDPGALNKD
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
KSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEENNKTTRQKYNENLKNRKEI
CCCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHH
ISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVK
HCCCCCEEEEEEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PRDDIYLLKDNNIPSVLIECGFLSNEKECKLLTDETYQEKIAWAIYIGIQKYLS
CCCCEEEEECCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]