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The map label for this gene is acoL [H]

Identifier: 126697605

GI number: 126697605

Start: 61659

End: 63389

Strand: Direct

Name: acoL [H]

Synonym: CD0039

Alternate gene names: 126697605

Gene position: 61659-63389 (Clockwise)

Preceding gene: 126697604

Following gene: 126697606

Centisome position: 1.44

GC content: 32.06

Gene sequence:

>1731_bases
ATGTCAGTAGAAGTAATAATGCCAAAAGCAGGAGTAGCCATGGAAGAAGGTACTATAGTATCTTGGCTAAAGCAAGAAGG
CGAAGAAGTAAAGATTGGAGAACCTATACTAGAAATAACAACAGATAAAGTTAATATGGAGATAGAGTCAGAAGGAGAAG
GTACACTTGCTGTAATCATACATAAAGAGGAAGGTGAAGTATTACCAGTATTTACAGTAATAGGTGTAATAGCAGAAAAA
GGGGAAAATCAAGAGGAAGTAAAAGCTAAATATTTATCTGGAAATGTTTCAAAAGAAGATACAGTTAAAGAGAATCAAAA
TATAGAAGTTAAAGAAGAAAAAATAAACAAAAAAGAGTGTAATCATGATTATGATGTAGTGGTAATAGGAGGAGGACCTG
GGGGATATTTATCAGCATTAAAAGCAGCTCTTTTAGGTGGAAGAGTTGCTTTAGTTGAAGAAAATATACTAGGAGGTACT
TGCTTAAATAGAGGTTGTATCCCTACAAAGACTTATATAAAAACAGCAGAAATATTAGAAGAAATTGACCAATTATCAAA
GAGAGGTGTAAAAGTAACTGTAGATAAAGAACAGGACATTAAGAAGGCCATAAAATATAAAAATAGAGTAGTTAAAAAAC
TTACAGCTGGTGTTGGCGGATTATTAAAAAGTAGAGATGTAGATGTGTTTAATCTTAAAGCTAGTGTTAAAGAAGAGCAT
AAAGTAATTTTATCAGATGGAAAAGTTTTAGATACAGAAAATATAATAATTGCTACAGGTTCCAAAGTAAGGATATTACC
TATAAAAGGAATAGAATCAAATTTAATAATAACTAGTACAGAAGCATTAGATTTGGAAACAGTGCCAGAAGAATTAGTAA
TAATTGGTGGTGGAGTGATTGGTTGTGAATTTGCAGAGATATTCAATTCAAGAGGTTCTAAAGTAACTATAGTAGAAATG
GAAGATAGAGTAATCCCAAGAATGGATAAAGAGTTAAGCGAATCTTTAAAATATTCTCTTAGTAAAAAAGGTATAAATGT
ATTAACTAAAAAGAAAGTTTCTGAATTTAAAGAAGAAGGAAATAACATATTAGTGTGCATTGAAGGTGAAGAGCCAATAA
AAGCAGATTTATGTCTATATGCTATAGGAAGAGAAGCTAATCTTTCTGGCATAGAGGACTTAGATATTAAAATAGATAAA
GGTTCAATAGTTGTAAATAGTAAAATGGAAACAAGTATACCAAGCATATATGCAGTAGGTGATGTTACTGGAGGTGTAAT
GTTAGCTCATGCTGCTTTTAAAATGGGAGAGGTAGCAGCTTCAAATGCTCTAGGTGTGAATAAAGAAGTTGACTTAGGTG
CATTACCTAGTTGTGTTTATACAATTCCAGAAGTAGCATCAGTTGGTATTACAGAAGAGGATGCTAGAAAAAAATATAAT
GTAAAAGTTGGTAAGTTTAATTTTGCTGGAAATGGAAGAGCTTTAGCTTCTGGTCAAGAACAAGGATATGTGAAGGTAGT
AGCAGATGCTAAATATGGAGAAATTTTAGGTATACATATGTTTGGATGTGGTGTAGCAGAACTTATAAATCATGCAGCAT
CATTTAAAGCATTAGAGATACCTACTGATGAAGCAAGTGAGTTGATATTTGGTCATCCATGTACTTCAGAAGCTTTAATG
GAAGCTTTGGCAGATGTAAATGGAGAATGTTTACACTTACCTAAAAAATAA

Upstream 100 bases:

>100_bases
ATTTAAAGCTTTTATATATTAAGAATTATTTTTAACAGTAACTATATGTGAATATGATTTGCTTAATTAAAATATAAGAA
AATAAATAGGAGGAGAGCCT

Downstream 100 bases:

>100_bases
ATCTAAACATAAATTATTAATTTAAGTCTATTATTAATATTAATTTATATCATAAAATTTTAAAACACTTACTTAGCCAT
ACCCCTTATTATGCAAATAA

Product: E3 component of acetoin dehydrogenase enzyme system (dihydrolipoyl dehydrogenase)

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of acetoin cleaving system [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKVNMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEK
GENQEEVKAKYLSGNVSKEDTVKENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGVGGLLKSRDVDVFNLKASVKEEH
KVILSDGKVLDTENIIIATGSKVRILPIKGIESNLIITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEM
EDRVIPRMDKELSESLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIEDLDIKIDK
GSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEVDLGALPSCVYTIPEVASVGITEEDARKKYN
VKVGKFNFAGNGRALASGQEQGYVKVVADAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALM
EALADVNGECLHLPKK

Sequences:

>Translated_576_residues
MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKVNMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEK
GENQEEVKAKYLSGNVSKEDTVKENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGVGGLLKSRDVDVFNLKASVKEEH
KVILSDGKVLDTENIIIATGSKVRILPIKGIESNLIITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEM
EDRVIPRMDKELSESLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIEDLDIKIDK
GSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEVDLGALPSCVYTIPEVASVGITEEDARKKYN
VKVGKFNFAGNGRALASGQEQGYVKVVADAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALM
EALADVNGECLHLPKK
>Mature_575_residues
SVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKVNMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKG
ENQEEVKAKYLSGNVSKEDTVKENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGTC
LNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGVGGLLKSRDVDVFNLKASVKEEHK
VILSDGKVLDTENIIIATGSKVRILPIKGIESNLIITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEME
DRVIPRMDKELSESLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIEDLDIKIDKG
SIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEVDLGALPSCVYTIPEVASVGITEEDARKKYNV
KVGKFNFAGNGRALASGQEQGYVKVVADAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALME
ALADVNGECLHLPKK

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=37.6906318082789, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=30.4347826086957, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI22035672, Length=448, Percent_Identity=30.1339285714286, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI291045266, Length=481, Percent_Identity=25.987525987526, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI33519430, Length=476, Percent_Identity=25, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI33519428, Length=476, Percent_Identity=25, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI33519426, Length=476, Percent_Identity=25, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI148277065, Length=476, Percent_Identity=25, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI148277071, Length=476, Percent_Identity=25, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI291045268, Length=364, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI65787454, Length=238, Percent_Identity=25.6302521008403, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI21389617, Length=238, Percent_Identity=25.6302521008403, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI226437568, Length=238, Percent_Identity=25.6302521008403, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786307, Length=465, Percent_Identity=35.6989247311828, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI87082354, Length=423, Percent_Identity=31.4420803782506, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=30.3964757709251, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=28.3062645011601, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789065, Length=233, Percent_Identity=25.7510729613734, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788892, Length=193, Percent_Identity=27.4611398963731, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=36.3238512035011, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=27.3469387755102, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=379, Percent_Identity=29.287598944591, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=379, Percent_Identity=29.287598944591, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=416, Percent_Identity=24.7596153846154, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=35.3684210526316, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=31.0924369747899, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=480, Percent_Identity=27.7083333333333, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=37.9912663755458, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24640551, Length=480, Percent_Identity=28.3333333333333, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=27.0833333333333, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=27.2340425531915, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.0960334029228, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 62055; Mature: 61924

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKVNMEIESEGEGTLAVII
CCEEEEECCCCCEECCCHHHHHHHHCCCEEECCCCEEEEEECEEEEEEECCCCCEEEEEE
HKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTVKENQNIEVKEEKINKKEC
ECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHCCHHHC
NHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGTCLNRGCIPTKTYIKTAEILE
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCHHCCCCCCCCHHHHHHHHHHH
EIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGVGGLLKSRDVDVFNLKASVKEEH
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
KVILSDGKVLDTENIIIATGSKVRILPIKGIESNLIITSTEALDLETVPEELVIIGGGVI
EEEEECCCEEECCCEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEECCEE
GCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSESLKYSLSKKGINVLTKKKVSEFKEEG
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
NNILVCIEGEEPIKADLCLYAIGREANLSGIEDLDIKIDKGSIVVNSKMETSIPSIYAVG
CEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCEEEEC
DVTGGVMLAHAAFKMGEVAASNALGVNKEVDLGALPSCVYTIPEVASVGITEEDARKKYN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCHHHHHCCCCCHHHHHEEE
VKVGKFNFAGNGRALASGQEQGYVKVVADAKYGEILGIHMFGCGVAELINHAASFKALEI
EEEEEEEECCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHCCEEEEC
PTDEASELIFGHPCTSEALMEALADVNGECLHLPKK
CCCCCCCEEECCCCCHHHHHHHHHCCCCCEEECCCC
>Mature Secondary Structure 
SVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKVNMEIESEGEGTLAVII
CEEEEECCCCCEECCCHHHHHHHHCCCEEECCCCEEEEEECEEEEEEECCCCCEEEEEE
HKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTVKENQNIEVKEEKINKKEC
ECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHCCHHHC
NHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGTCLNRGCIPTKTYIKTAEILE
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCHHCCCCCCCCHHHHHHHHHHH
EIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGVGGLLKSRDVDVFNLKASVKEEH
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
KVILSDGKVLDTENIIIATGSKVRILPIKGIESNLIITSTEALDLETVPEELVIIGGGVI
EEEEECCCEEECCCEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEECCEE
GCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSESLKYSLSKKGINVLTKKKVSEFKEEG
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
NNILVCIEGEEPIKADLCLYAIGREANLSGIEDLDIKIDKGSIVVNSKMETSIPSIYAVG
CEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCEEEEC
DVTGGVMLAHAAFKMGEVAASNALGVNKEVDLGALPSCVYTIPEVASVGITEEDARKKYN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCHHHHHCCCCCHHHHHEEE
VKVGKFNFAGNGRALASGQEQGYVKVVADAKYGEILGIHMFGCGVAELINHAASFKALEI
EEEEEEEECCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHCCEEEEC
PTDEASELIFGHPCTSEALMEALADVNGECLHLPKK
CCCCCCCEEECCCCCHHHHHHHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]