Click here to switch to the map view.

The map label for this gene is mdlY [H]

Identifier: 262192883

GI number: 262192883

Start: 359739

End: 361103

Strand: Reverse

Name: mdlY [H]

Synonym: BMA10247_A0409

Alternate gene names: 262192883

Gene position: 361103-359739 (Counterclockwise)

Preceding gene: 126445835

Following gene: 126446483

Centisome position: 15.35

GC content: 73.48

Gene sequence:

>1365_bases
GTGCGAGCCATCGCGGATGCCGTGAACGGGAGGCGGGTGCGCGCGCTCGAGCAGGCCGAGGCGGCGCTCGAACGTCTCGC
GCTCGTCGAGCCGCTCGCGGCGATGACGCACGTCGCGCCGGAGATCGCGCGCCGGGCCGCCGCGAACGTCGACGCGCAGA
TCGCGAGCGGCCGCCGGCTGCCGCTCGCGGGCGTGCCGTTCGTCATCAAGGACAACCTCTTCACCGCCGACATGCCGACG
CGCGCGGCCAGCCCCGCGCTGCTCGGCTTCGACGCGACGCGCAACGCGACGGCCGTCCAGCAGTTGCTCGACGCGGGCGC
CGTGCCGCTCGGCAAGGCGAACATGCACGAGCTCGCGTTCGGCATCACGAGCGCGAACGGCTACTTCGGCGCGGTGCGTA
ATCCGCACGACCCGGCGCGCATCGCGGGCGGCTCGAGCGGCGGCACGGCGTGCGCGGTCGCGGCGGGCATCGCGTTCGGC
CTCGGCACCGACACGGGCGGCTCGGTGCGCATCCCGGCCGCGTTTTGCGGCGTCGCCGGATTGCGGCCGACGCCGCGCCG
CTACTCGACGGAAGGCGTACTCGCGCTATCGCCGACGCGCGATACGGTCGGCCCGATCGCGCACGGCATCGACGATTTGC
TGCTGCTCGACGCCGTGCTGACGGGCGACGCCGGCGAGCCCGCACCGGTCGCGCTCGCCGGCTTGCGCGTCGGCGTGCCG
GATGCGCCCTACTTCGACGGGCTCGACCCGGCCGTCGCGGAACTGACCGAGCGCGCACTCGCCGCGCTGACGGACGCGGG
CGCGCGACTGATCCCGCTCGCCGCCCACGCGCTGCACGAAGCGGACGAAGCCTGCGGCTTCCCGATCGCCGGGTTCGAGG
CGCTCGCGTACTGGCGCCGCTTCAGCGCCGAACGGCTCGATCTGCCGTTCGCGGAATTCGTCGCCCGGCTCGGCAGCGAC
GATGTGCGCCACCTCTTCCGCATGCTGATCGACGCACCGCCGTCGAGCGATGCGTATGCGGCCGCGATCGACGTGCTGCA
AAGCACCGTGCGCTGGTACGACCAGGCGTTCCATCGGACGGGCGTCGATTGCATCGCGCTGCCGACCGTTCAATGCGACG
CGCCGCTCGTCACCGACAGCGGGCACGATCTTCCTTCCGACGCCGCGTACGCGCTGTTCGCGCGCGTGATCCGCCAGACC
GGGCCGGCGACGCTCGGCGGCGTGCCCTCGGTATCGATTCCGGCGGGATTGTCCAAGCACACCGGCGTGCCCGTGGGCCT
GATGCTCGAAGGGCCGGCAGGCAACGACCGGCGCCTGCTCGCGATCGCGCGCGAGGTGGAGCGGCTGCTCGCGCGGGCGC
CGTGA

Upstream 100 bases:

>100_bases
GATCGAGGCGACAACGCACGCCGCGCGTCGCGGCGCGCCACACACATCAGGAGACAGGTCAATGACGTCACATCGGGATA
CCTACGTCCGGTCCGCCAGC

Downstream 100 bases:

>100_bases
AGCGCGCGTGCTTGCCTGGCGCTCGTGTGACGCTCATCCGAGGTTCATTTGACGCTCGTGTGACGCGCCTGTGGTGCTCG
CCCCGCGCGGGTCTCGCGCA

Product: indole acetimide hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MRAIADAVNGRRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRLPLAGVPFVIKDNLFTADMPT
RAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSGGTACAVAAGIAFG
LGTDTGGSVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEPAPVALAGLRVGVP
DAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRRFSAERLDLPFAEFVARLGSD
DVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSDAAYALFARVIRQT
GPATLGGVPSVSIPAGLSKHTGVPVGLMLEGPAGNDRRLLAIAREVERLLARAP

Sequences:

>Translated_454_residues
MRAIADAVNGRRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRLPLAGVPFVIKDNLFTADMPT
RAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSGGTACAVAAGIAFG
LGTDTGGSVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEPAPVALAGLRVGVP
DAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRRFSAERLDLPFAEFVARLGSD
DVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSDAAYALFARVIRQT
GPATLGGVPSVSIPAGLSKHTGVPVGLMLEGPAGNDRRLLAIAREVERLLARAP
>Mature_454_residues
MRAIADAVNGRRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRLPLAGVPFVIKDNLFTADMPT
RAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSGGTACAVAAGIAFG
LGTDTGGSVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEPAPVALAGLRVGVP
DAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRRFSAERLDLPFAEFVARLGSD
DVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSDAAYALFARVIRQT
GPATLGGVPSVSIPAGLSKHTGVPVGLMLEGPAGNDRRLLAIAREVERLLARAP

Specific function: Hydrolyzes both the R- and the S-enantiomers of mandelamide, and phenylacetamide. Has lower activity on 3- phenylpropionaide and lactamide. Does not hydrolyze benzamide. Hydrolyzes esters and amides with little steric bulk. Preferentially hydrolyzes aroma

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=28.8270377733598, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI195972892, Length=233, Percent_Identity=30.9012875536481, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=472, Percent_Identity=27.5423728813559, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=235, Percent_Identity=31.063829787234, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=488, Percent_Identity=27.4590163934426, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=32.620320855615, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=175, Percent_Identity=32.5714285714286, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=157, Percent_Identity=37.5796178343949, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=157, Percent_Identity=37.5796178343949, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=171, Percent_Identity=30.4093567251462, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=439, Percent_Identity=27.5626423690205, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319685, Length=154, Percent_Identity=37.012987012987, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648113, Length=477, Percent_Identity=31.6561844863732, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=28.6298568507157, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648435, Length=480, Percent_Identity=26.0416666666667, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648437, Length=480, Percent_Identity=26.0416666666667, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45550774, Length=480, Percent_Identity=26.0416666666667, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652985, Length=499, Percent_Identity=25.8517034068136, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922090, Length=499, Percent_Identity=25.8517034068136, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652981, Length=499, Percent_Identity=25.8517034068136, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652983, Length=499, Percent_Identity=25.8517034068136, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24644968, Length=436, Percent_Identity=24.0825688073394, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=414, Percent_Identity=25.8454106280193, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648439, Length=414, Percent_Identity=25.8454106280193, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=493, Percent_Identity=25.5578093306288, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.86 [H]

Molecular weight: Translated: 47085; Mature: 47085

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAIADAVNGRRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRL
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCC
PLAGVPFVIKDNLFTADMPTRAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAF
CCCCCCEEEECCEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHE
GITSANGYFGAVRNPHDPARIAGGSSGGTACAVAAGIAFGLGTDTGGSVRIPAAFCGVAG
EEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCHHHHCCCC
LRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEPAPVALAGLRVGVP
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
DAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FSAERLDLPFAEFVARLGSDDVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRT
HCHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GVDCIALPTVQCDAPLVTDSGHDLPSDAAYALFARVIRQTGPATLGGVPSVSIPAGLSKH
CCCEEEECCEECCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TGVPVGLMLEGPAGNDRRLLAIAREVERLLARAP
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAIADAVNGRRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRL
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCC
PLAGVPFVIKDNLFTADMPTRAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAF
CCCCCCEEEECCEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHE
GITSANGYFGAVRNPHDPARIAGGSSGGTACAVAAGIAFGLGTDTGGSVRIPAAFCGVAG
EEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCHHHHCCCC
LRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEPAPVALAGLRVGVP
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
DAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FSAERLDLPFAEFVARLGSDDVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRT
HCHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GVDCIALPTVQCDAPLVTDSGHDLPSDAAYALFARVIRQTGPATLGGVPSVSIPAGLSKH
CCCEEEECCEECCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TGVPVGLMLEGPAGNDRRLLAIAREVERLLARAP
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12670968 [H]