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The map label for this gene is fdoH [H]

Identifier: 126447842

GI number: 126447842

Start: 521683

End: 522597

Strand: Direct

Name: fdoH [H]

Synonym: BMA10247_A0572

Alternate gene names: 126447842

Gene position: 521683-522597 (Clockwise)

Preceding gene: 126446879

Following gene: 126447040

Centisome position: 22.17

GC content: 66.12

Gene sequence:

>915_bases
ATGGCATTGCAATCGCTCGATATCAAGCGCGTCTCGGCCACCACGACGCCTCCTCCCACCGCGCGCGAGCCGGTGACGGG
CAGCGTCGCGAAGCTGATCGACGTATCGAAATGCATCGGCTGCAAGGCATGTCAGACCGCCTGCATGGAGTGGAACGACC
TGCGCGACGAAGTCGGCACGAACGTCGGCGTCTACGACAATCCGGCCGATCTGACCGAGCACTCGTGGACGGTGATGCGT
TTCGCCGAATACGAGAACCCGGCGGGCGACCTCGAATGGCTGATCCGCAAGGACGGCTGCATGCACTGCGAGGACCCGGG
CTGCCTGAAGGCGTGCCCTTCGCCGGGCGCGATCGTGCAGTACAACAACGGGATCGTCGATTTCCACGAGGAGCACTGCA
TCGGCTGCGGCTACTGCGTGACCGGCTGCCCGTTCAACATCCCGCGGATCTCGAAGCAGGACAACCGCGCGTACAAGTGC
ACGCTTTGCTCGGACCGCGTCGCGGTCGGCCAGGAGCCCGCGTGCGTGAAGACCTGCCCGACGGGCGCGATCGTGTTCGG
CACGAAGGAGGACATGAAGCAGCACGCGGCCGAGCGTGTCGCCGATCTGAAGGGGCGCGGCTTCGAGAACGCGGGGCTGT
ACGATCCGCAGGGCGTCGGCGGCACGCATGTGATGTACGTGCTGCATCACGCGGACAAGCCGTCGCTGTATCACGGCCTG
CCCGACAATCCGTCGATCAGCCCGATGGTGAAGCTGTGGAAGGGGCTCGCGAAGCCGCTCGCGGTTGCGGGCATCGCGCT
GTCCGCGCTCGTCGGCTTCTTCCACTACACGCGCGTCGGCCCGAACGACGTGACGGACGACGAGGAGGCGGCCGCCCGTG
ACGAAGCGCGCCGCATCCAGGAGGACGCGAAATGA

Upstream 100 bases:

>100_bases
CTCGCGAACACCCTGACTCCGTCCGTCGGCGACGGCAACTCGCAGACACCGGAATTCAAATCGTTCCTGGTGAAAGTGGA
AAAGGCGTAAGGGGAAAGAG

Downstream 100 bases:

>100_bases
AACATGACGACCCGAACCTGATCGTCCGCTACACGGCGAACGAGCGCTCGAACCACTGGATCACCGTGATCTCGTTCGTG
CTGCTCGCGCTGTCCGGGCT

Product: formate dehydrogenase, beta subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEVGTNVGVYDNPADLTEHSWTVMR
FAEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEEHCIGCGYCVTGCPFNIPRISKQDNRAYKC
TLCSDRVAVGQEPACVKTCPTGAIVFGTKEDMKQHAAERVADLKGRGFENAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
PDNPSISPMVKLWKGLAKPLAVAGIALSALVGFFHYTRVGPNDVTDDEEAAARDEARRIQEDAK

Sequences:

>Translated_304_residues
MALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEVGTNVGVYDNPADLTEHSWTVMR
FAEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEEHCIGCGYCVTGCPFNIPRISKQDNRAYKC
TLCSDRVAVGQEPACVKTCPTGAIVFGTKEDMKQHAAERVADLKGRGFENAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
PDNPSISPMVKLWKGLAKPLAVAGIALSALVGFFHYTRVGPNDVTDDEEAAARDEARRIQEDAK
>Mature_303_residues
ALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEVGTNVGVYDNPADLTEHSWTVMRF
AEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEEHCIGCGYCVTGCPFNIPRISKQDNRAYKCT
LCSDRVAVGQEPACVKTCPTGAIVFGTKEDMKQHAAERVADLKGRGFENAGLYDPQGVGGTHVMYVLHHADKPSLYHGLP
DNPSISPMVKLWKGLAKPLAVAGIALSALVGFFHYTRVGPNDVTDDEEAAARDEARRIQEDAK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=302, Percent_Identity=68.2119205298013, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1787749, Length=291, Percent_Identity=68.7285223367698, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1789370, Length=305, Percent_Identity=29.1803278688525, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787122, Length=179, Percent_Identity=35.195530726257, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787872, Length=172, Percent_Identity=36.046511627907, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI2367345, Length=159, Percent_Identity=32.0754716981132, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI226510944, Length=163, Percent_Identity=30.6748466257669, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788811, Length=179, Percent_Identity=28.4916201117318, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787740, Length=169, Percent_Identity=28.9940828402367, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082179, Length=173, Percent_Identity=27.7456647398844, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787963, Length=149, Percent_Identity=26.8456375838926, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=27.710843373494, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI2367245, Length=174, Percent_Identity=29.3103448275862, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787478, Length=169, Percent_Identity=27.2189349112426, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33026; Mature: 32895

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEVGT
CCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHCC
NVGVYDNPADLTEHSWTVMRFAEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCCCCEEEEHHCCCCCCCHHHHHHCCCCCCCCCCCHHCCCCCCCCEEE
YNNGIVDFHEEHCIGCGYCVTGCPFNIPRISKQDNRAYKCTLCSDRVAVGQEPACVKTCP
ECCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCEEEECC
TGAIVFGTKEDMKQHAAERVADLKGRGFENAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCC
PDNPSISPMVKLWKGLAKPLAVAGIALSALVGFFHYTRVGPNDVTDDEEAAARDEARRIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EDAK
HHCC
>Mature Secondary Structure 
ALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEVGT
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHCC
NVGVYDNPADLTEHSWTVMRFAEYENPAGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCCCCEEEEHHCCCCCCCHHHHHHCCCCCCCCCCCHHCCCCCCCCEEE
YNNGIVDFHEEHCIGCGYCVTGCPFNIPRISKQDNRAYKCTLCSDRVAVGQEPACVKTCP
ECCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCEEEECC
TGAIVFGTKEDMKQHAAERVADLKGRGFENAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCC
PDNPSISPMVKLWKGLAKPLAVAGIALSALVGFFHYTRVGPNDVTDDEEAAARDEARRIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EDAK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]