Click here to switch to the map view.

The map label for this gene is potD-A [H]

Identifier: 126447731

GI number: 126447731

Start: 431182

End: 432306

Strand: Direct

Name: potD-A [H]

Synonym: BMA10247_A0482

Alternate gene names: 126447731

Gene position: 431182-432306 (Clockwise)

Preceding gene: 126447057

Following gene: 126447505

Centisome position: 18.33

GC content: 64.71

Gene sequence:

>1125_bases
ATGAACGCGAAGCCGCGCGTGGCGCGCCGCGCTTCGTATAACCACCACGCTTCATCATCCGTCACGAGAGGAAACATCAT
GCACCACACGTTCAACACGCCGCGCCGCGCGGTTGCGCTGCTGTCGCTCGCCGCGTTCTGCGCGACGGCCGGCGCGGCCG
AGCTGACCGTCGTCAATTTCGGCGGCGCGAACGGCGACGCGCAGAAGGCCGCGTTCAACCAGCCGTTCGAGAAGGCGACC
GGCAACAAGGTGACGGCCGTCGAATACAACGGCGAGCAGGCGAAAGTGAAGGCGATGGTCGAGGCGAAGCACGTGAACTG
GGACGTCGTCGAAGTCGAGTCGGGCGACCTGAACCGCGGCTGCGACGAAGGCCTGTACGAGAAACTCGACTGGGCGAAGA
TCGCGAAGAAGTCCGATCTGATTGCCGAATCGCCGCAAACCTGCGGCGTCGGTTTCTTCGTGTGGTCGACCGCGCTTGCA
TACGACGCGGACAAGCTCAAGCGCGCGCCCGCCGGCTGGGCCGATTTCTGGGACGTGAAGAAATTCCCGGGCAAGCGCGG
GATGCACAAGGGCGCGCGCTACAACCTCGAGTTCGCGCTGATGGCGGACGGCGTCGCGCCGAAGGACGTCTACAAGGTGC
TCGCGACGAAGGCGGGCCAGGATCGCGCATTCAAGAAGCTTGACGAGCTGAAGCCGAACATCCAGTGGTGGGAAGCGGGC
GCGCAGCCGCCGCAGTTCCTCGTCGCGGGCGACGTCGTGATGTCGACCGCGTACAACGGCCGCATCAGCGCCGCGCAGAA
GGAAGGCAAGAACCTGAAGGTGGTGTGGAGCGGCAGCATCTACGATCTGGACTACTGGGCGATTCCGAAGGGCACGCCGA
ACAAGGCGCTCGCCGAGAAGTACATCGCCTACACGCTGTCGTCGAAGCCGCAGCAGGATTACGCGCAACACATCGCGTAC
GGCCCCGCGAATGTCGCCGCGATCAAGGCGCTCGACGCGAAGACGCTCGCGAACCTGCCGAACTCGCCGGCCAACGGCAA
GAACGCGGTGCTGCAGAACATCACGTTCTGGACCGATCACGGCGACGAGCTCGAGCAGCGCTTTGCGGCATGGGCGTCGA
AGTGA

Upstream 100 bases:

>100_bases
GCTTCGTCAAGGTGCCGCTCGGCACCGGCGCGCTCGATGCGTTCGCGCCCGGCGTGCCGATCTCGCTCGAGTTTCAGCCC
GAGCATCTGCGGGTCTTCGC

Downstream 100 bases:

>100_bases
CGGCCGCGTGAGCCGGTGAAGCAAACGGCCGCGCGCGCCGTGGCGGCGTGCGCGACGTTCCTCCGAACGGCGTTTCGGCC
GGAGACAACCGTTGAATACG

Product: spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MNAKPRVARRASYNHHASSSVTRGNIMHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKAT
GNKVTAVEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALA
YDADKLKRAPAGWADFWDVKKFPGKRGMHKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAG
AQPPQFLVAGDVVMSTAYNGRISAAQKEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAY
GPANVAAIKALDAKTLANLPNSPANGKNAVLQNITFWTDHGDELEQRFAAWASK

Sequences:

>Translated_374_residues
MNAKPRVARRASYNHHASSSVTRGNIMHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKAT
GNKVTAVEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALA
YDADKLKRAPAGWADFWDVKKFPGKRGMHKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAG
AQPPQFLVAGDVVMSTAYNGRISAAQKEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAY
GPANVAAIKALDAKTLANLPNSPANGKNAVLQNITFWTDHGDELEQRFAAWASK
>Mature_374_residues
MNAKPRVARRASYNHHASSSVTRGNIMHHTFNTPRRAVALLSLAAFCATAGAAELTVVNFGGANGDAQKAAFNQPFEKAT
GNKVTAVEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRGCDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALA
YDADKLKRAPAGWADFWDVKKFPGKRGMHKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAG
AQPPQFLVAGDVVMSTAYNGRISAAQKEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEKYIAYTLSSKPQQDYAQHIAY
GPANVAAIKALDAKTLANLPNSPANGKNAVLQNITFWTDHGDELEQRFAAWASK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=274, Percent_Identity=25.5474452554745, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40854; Mature: 40854

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKPRVARRASYNHHASSSVTRGNIMHHTFNTPRRAVALLSLAAFCATAGAAELTVVNF
CCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
GGANGDAQKAAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRG
CCCCCCHHHHHHCCCHHHCCCCEEEEEEECCCHHEEEEEEEHCCCCEEEEEECCCCCCCC
CDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALAYDADKLKRAPAGWADFWDVK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHH
KFPGKRGMHKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAG
HCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECC
AQPPQFLVAGDVVMSTAYNGRISAAQKEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEK
CCCCCEEEECHHHHHHCCCCCEEHHHHCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHH
YIAYTLSSKPQQDYAQHIAYGPANVAAIKALDAKTLANLPNSPANGKNAVLQNITFWTDH
HHHHEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEECCC
GDELEQRFAAWASK
CHHHHHHHHHHCCC
>Mature Secondary Structure
MNAKPRVARRASYNHHASSSVTRGNIMHHTFNTPRRAVALLSLAAFCATAGAAELTVVNF
CCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
GGANGDAQKAAFNQPFEKATGNKVTAVEYNGEQAKVKAMVEAKHVNWDVVEVESGDLNRG
CCCCCCHHHHHHCCCHHHCCCCEEEEEEECCCHHEEEEEEEHCCCCEEEEEECCCCCCCC
CDEGLYEKLDWAKIAKKSDLIAESPQTCGVGFFVWSTALAYDADKLKRAPAGWADFWDVK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHH
KFPGKRGMHKGARYNLEFALMADGVAPKDVYKVLATKAGQDRAFKKLDELKPNIQWWEAG
HCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECC
AQPPQFLVAGDVVMSTAYNGRISAAQKEGKNLKVVWSGSIYDLDYWAIPKGTPNKALAEK
CCCCCEEEECHHHHHHCCCCCEEHHHHCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHH
YIAYTLSSKPQQDYAQHIAYGPANVAAIKALDAKTLANLPNSPANGKNAVLQNITFWTDH
HHHHEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEECCC
GDELEQRFAAWASK
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]