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The map label for this gene is alkK [H]

Identifier: 126447266

GI number: 126447266

Start: 2256863

End: 2258668

Strand: Reverse

Name: alkK [H]

Synonym: BMA10247_A2328

Alternate gene names: 126447266

Gene position: 2258668-2256863 (Counterclockwise)

Preceding gene: 126446635

Following gene: 126447061

Centisome position: 96.0

GC content: 69.77

Gene sequence:

>1806_bases
GTGCACGCGACCGATCAGCATTCCGCCTTGAACGCCGCCCCGTCGGCCTACGTCTATCCGCTGCTCGTCGGGCAGTTGCT
GACGCACGCGCGCGGTGCCGACGCTGGCCGACAGATCGTCTATCGCGGCGACGTGCGCCATTCGTATGCGCAGTTTCGCG
AGCGCATCGGTCGGCTTGCCGGCGCGCTCGCGGCGCTCGGCGTCGGCGCGGACGCGACCGTCGCGGTGATGGACTGGGAC
AGCCATCGCTATCTGGAAAGTTACTTCGCTATCCCGATGATGGGCGCGACGCTGTTCACGGTGAACGTGCGACTCTCGCC
GCGGCAGATCGCCCATACGCTGCGCGACGCCCGCGCGTCGGTCGTTGTCGCGCATGCGGATTTCCTGCCGCTGCTCGAAC
AGGTGCGCGATGCGCTCGGCGATAGCACGTGCGTGATCGTCGCGTGCGACGGTGGCGCGATGCCGGCGACGTCGCTGCCG
CTCGCGGGCGAATACGAGCGGCTCGTCGCCGCCGCCGACCCGGATTACCCGTTCGCCGATTTCGACGAGAACGCGCGCGC
GGTGCTGTTCTACACGACGGGCACGACCGGCGATCCGAAGGGCGTCTGCTATAGCCACCGGCAGATCGTGCTGCACGCGC
TCGCGACCGCCGCTGCGCTCGGCGCGGCGCGCGATGGCCAGCGGCTGCATCGCGACGACGTCTACATGCCGATCACGCCG
ATGTTTCATGTGATGGCGTGGGGCATGCCGTACATCGCGGTGATGCTCGGCCTGAAGATCGTGCTGCCGGGGCGCTACCG
CGCGCACGCGCTGCTCGACCTGCGGCAAGCCGAGCGCGTCACGTTCTCGCACTGCGTGCCGGCGGTGCTGCAGATGCTGC
TCGACGAGGCGCGGCTGCGCGCGTGCGATCTGTCTGGCTGGAAGATGATCGTCGGCGGCTCGGCGCTGCCGGCTTCGCTG
TGCCGGGCCGCGCGCGCGCGGCGCATCGACGTGTTCGCCGGCTACGGGATGTCGGAAACCGGGCCGGTGGTCGCGCTCGC
GCAGTTGCGAGGCACGGAGGGGGGCGTCGATGTCCATGCCGATGAGGATGCCGGTATCGATGCTGATGTCGGCGCCGGTA
TCGATGTCGATACCGATGTCGACGCGGATATCAATGCCAATGCCGGCGCCAACGCGTCCGATTCATCCGTCACGACCGCC
GCCGCGCACGCGGACGACGAAACCGCCAAGCGCTGCGCCGCCGGCTGGCCGCCGCCGCTCGTCGAGCTGCGCGTCGTCGA
CGCAGCGATGCGCGATGTGCCGCGCGACGGCCGCACCCCGGGCGAAATCGTATTGCGCGGGCCGGCGCTCGCGCTCGGCT
ATCGTGGCAATCCGCAGGCGAGCGCCGCCTTGTGGGCGGGCGGCTATCTGCATACACAGGACGTCGCGGTGATGCACGGC
GACGGTGCGATCCGGATCGTCGACCGCATCAAGGACGTGATCAAGACGGGCGGCGAATGGGTGTCGTCGCTCGAGCTGGA
AGGGCTGCTGAACGATATCGCGGGCATTCGGGAGAGCGCGGTGGTCGGCGTGCCGGACGCGCGCTGGGGCGAGCGCCCGG
CCGCTGTCGTCGTGTGCGAGCGCGACGCGCCGCTCGCCGCGCAGGACGTGCGCGCGCGGCTGCTCGAGCACGTCGCCGCG
AACCGCATCAGCCGCTATGCGGTGCCGGAGTGCGAGCGCATCGTGTTCGTCGACGCGCTGCCGAAGACAAGCGTCGGCAA
GATCGACAAGAAATCGCTGCGGCGCCTCGTCGAGCGGATCGACTGA

Upstream 100 bases:

>100_bases
CCCCCTCGCGGTTGGGGGGCAGACGCCCGGCGCGGCTGCGTATCCTCACTCACGCGATGCGATTTCCCGCATCGACGCAC
TCAACGAGAGGAGCCGCCCC

Downstream 100 bases:

>100_bases
CGCGGTCGCAACACGGCCACCGTCAGCCTCGATGCTGCCGGGCCATTTCCCCGACATGGGAGACATGATGGGAGACATGA
TGAACGCGAAATCGATCGCG

Product: medium-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MHATDQHSALNAAPSAYVYPLLVGQLLTHARGADAGRQIVYRGDVRHSYAQFRERIGRLAGALAALGVGADATVAVMDWD
SHRYLESYFAIPMMGATLFTVNVRLSPRQIAHTLRDARASVVVAHADFLPLLEQVRDALGDSTCVIVACDGGAMPATSLP
LAGEYERLVAAADPDYPFADFDENARAVLFYTTGTTGDPKGVCYSHRQIVLHALATAAALGAARDGQRLHRDDVYMPITP
MFHVMAWGMPYIAVMLGLKIVLPGRYRAHALLDLRQAERVTFSHCVPAVLQMLLDEARLRACDLSGWKMIVGGSALPASL
CRAARARRIDVFAGYGMSETGPVVALAQLRGTEGGVDVHADEDAGIDADVGAGIDVDTDVDADINANAGANASDSSVTTA
AAHADDETAKRCAAGWPPPLVELRVVDAAMRDVPRDGRTPGEIVLRGPALALGYRGNPQASAALWAGGYLHTQDVAVMHG
DGAIRIVDRIKDVIKTGGEWVSSLELEGLLNDIAGIRESAVVGVPDARWGERPAAVVVCERDAPLAAQDVRARLLEHVAA
NRISRYAVPECERIVFVDALPKTSVGKIDKKSLRRLVERID

Sequences:

>Translated_601_residues
MHATDQHSALNAAPSAYVYPLLVGQLLTHARGADAGRQIVYRGDVRHSYAQFRERIGRLAGALAALGVGADATVAVMDWD
SHRYLESYFAIPMMGATLFTVNVRLSPRQIAHTLRDARASVVVAHADFLPLLEQVRDALGDSTCVIVACDGGAMPATSLP
LAGEYERLVAAADPDYPFADFDENARAVLFYTTGTTGDPKGVCYSHRQIVLHALATAAALGAARDGQRLHRDDVYMPITP
MFHVMAWGMPYIAVMLGLKIVLPGRYRAHALLDLRQAERVTFSHCVPAVLQMLLDEARLRACDLSGWKMIVGGSALPASL
CRAARARRIDVFAGYGMSETGPVVALAQLRGTEGGVDVHADEDAGIDADVGAGIDVDTDVDADINANAGANASDSSVTTA
AAHADDETAKRCAAGWPPPLVELRVVDAAMRDVPRDGRTPGEIVLRGPALALGYRGNPQASAALWAGGYLHTQDVAVMHG
DGAIRIVDRIKDVIKTGGEWVSSLELEGLLNDIAGIRESAVVGVPDARWGERPAAVVVCERDAPLAAQDVRARLLEHVAA
NRISRYAVPECERIVFVDALPKTSVGKIDKKSLRRLVERID
>Mature_601_residues
MHATDQHSALNAAPSAYVYPLLVGQLLTHARGADAGRQIVYRGDVRHSYAQFRERIGRLAGALAALGVGADATVAVMDWD
SHRYLESYFAIPMMGATLFTVNVRLSPRQIAHTLRDARASVVVAHADFLPLLEQVRDALGDSTCVIVACDGGAMPATSLP
LAGEYERLVAAADPDYPFADFDENARAVLFYTTGTTGDPKGVCYSHRQIVLHALATAAALGAARDGQRLHRDDVYMPITP
MFHVMAWGMPYIAVMLGLKIVLPGRYRAHALLDLRQAERVTFSHCVPAVLQMLLDEARLRACDLSGWKMIVGGSALPASL
CRAARARRIDVFAGYGMSETGPVVALAQLRGTEGGVDVHADEDAGIDADVGAGIDVDTDVDADINANAGANASDSSVTTA
AAHADDETAKRCAAGWPPPLVELRVVDAAMRDVPRDGRTPGEIVLRGPALALGYRGNPQASAALWAGGYLHTQDVAVMHG
DGAIRIVDRIKDVIKTGGEWVSSLELEGLLNDIAGIRESAVVGVPDARWGERPAAVVVCERDAPLAAQDVRARLLEHVAA
NRISRYAVPECERIVFVDALPKTSVGKIDKKSLRRLVERID

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=580, Percent_Identity=25.5172413793103, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI187761343, Length=580, Percent_Identity=25.5172413793103, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI156151445, Length=213, Percent_Identity=32.8638497652582, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI42544132, Length=186, Percent_Identity=29.0322580645161, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI115511026, Length=209, Percent_Identity=28.7081339712919, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI28416953, Length=130, Percent_Identity=34.6153846153846, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788107, Length=574, Percent_Identity=25.9581881533101, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI145693145, Length=183, Percent_Identity=37.1584699453552, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI221142682, Length=256, Percent_Identity=26.171875, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786810, Length=194, Percent_Identity=34.020618556701, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=173, Percent_Identity=38.728323699422, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=609, Percent_Identity=22.824302134647, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=642, Percent_Identity=22.8971962616822, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=642, Percent_Identity=22.8971962616822, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=592, Percent_Identity=22.2972972972973, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=205, Percent_Identity=32.1951219512195, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=177, Percent_Identity=31.638418079096, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=179, Percent_Identity=28.4916201117318, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=565, Percent_Identity=20.8849557522124, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=156, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=155, Percent_Identity=29.6774193548387, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560140, Length=160, Percent_Identity=30.625, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32563687, Length=155, Percent_Identity=30.3225806451613, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=153, Percent_Identity=31.3725490196078, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319264, Length=136, Percent_Identity=33.8235294117647, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI21355181, Length=566, Percent_Identity=24.3816254416961, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581924, Length=412, Percent_Identity=25.7281553398058, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859661, Length=172, Percent_Identity=36.046511627907, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356947, Length=193, Percent_Identity=28.4974093264249, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI20130357, Length=147, Percent_Identity=27.891156462585, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653035, Length=188, Percent_Identity=27.6595744680851, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 64270; Mature: 64270

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHATDQHSALNAAPSAYVYPLLVGQLLTHARGADAGRQIVYRGDVRHSYAQFRERIGRLA
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
GALAALGVGADATVAVMDWDSHRYLESYFAIPMMGATLFTVNVRLSPRQIAHTLRDARAS
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHCCCE
VVVAHADFLPLLEQVRDALGDSTCVIVACDGGAMPATSLPLAGEYERLVAAADPDYPFAD
EEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHEEECCCCCCCCC
FDENARAVLFYTTGTTGDPKGVCYSHRQIVLHALATAAALGAARDGQRLHRDDVYMPITP
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHH
MFHVMAWGMPYIAVMLGLKIVLPGRYRAHALLDLRQAERVTFSHCVPAVLQMLLDEARLR
HHHHHHCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACDLSGWKMIVGGSALPASLCRAARARRIDVFAGYGMSETGPVVALAQLRGTEGGVDVHA
EECCCCCEEEECCCCCHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEC
DEDAGIDADVGAGIDVDTDVDADINANAGANASDSSVTTAAAHADDETAKRCAAGWPPPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCC
VELRVVDAAMRDVPRDGRTPGEIVLRGPALALGYRGNPQASAALWAGGYLHTQDVAVMHG
EEHHHHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCCCCCEEEEECCEEEECCEEEEEC
DGAIRIVDRIKDVIKTGGEWVSSLELEGLLNDIAGIRESAVVGVPDARWGERPAAVVVCE
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEC
RDAPLAAQDVRARLLEHVAANRISRYAVPECERIVFVDALPKTSVGKIDKKSLRRLVERI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHC
D
C
>Mature Secondary Structure
MHATDQHSALNAAPSAYVYPLLVGQLLTHARGADAGRQIVYRGDVRHSYAQFRERIGRLA
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
GALAALGVGADATVAVMDWDSHRYLESYFAIPMMGATLFTVNVRLSPRQIAHTLRDARAS
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHCCCE
VVVAHADFLPLLEQVRDALGDSTCVIVACDGGAMPATSLPLAGEYERLVAAADPDYPFAD
EEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHEEECCCCCCCCC
FDENARAVLFYTTGTTGDPKGVCYSHRQIVLHALATAAALGAARDGQRLHRDDVYMPITP
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHH
MFHVMAWGMPYIAVMLGLKIVLPGRYRAHALLDLRQAERVTFSHCVPAVLQMLLDEARLR
HHHHHHCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACDLSGWKMIVGGSALPASLCRAARARRIDVFAGYGMSETGPVVALAQLRGTEGGVDVHA
EECCCCCEEEECCCCCHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEC
DEDAGIDADVGAGIDVDTDVDADINANAGANASDSSVTTAAAHADDETAKRCAAGWPPPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCC
VELRVVDAAMRDVPRDGRTPGEIVLRGPALALGYRGNPQASAALWAGGYLHTQDVAVMHG
EEHHHHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCCCCCEEEEECCEEEECCEEEEEC
DGAIRIVDRIKDVIKTGGEWVSSLELEGLLNDIAGIRESAVVGVPDARWGERPAAVVVCE
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEC
RDAPLAAQDVRARLLEHVAANRISRYAVPECERIVFVDALPKTSVGKIDKKSLRRLVERI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]