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The map label for this gene is azoR [H]

Identifier: 126447153

GI number: 126447153

Start: 1847621

End: 1848334

Strand: Reverse

Name: azoR [H]

Synonym: BMA10247_A1916

Alternate gene names: 126447153

Gene position: 1848334-1847621 (Counterclockwise)

Preceding gene: 126445719

Following gene: 126446754

Centisome position: 78.56

GC content: 66.39

Gene sequence:

>714_bases
TTGTTTCGCTATTGCAACAATCCGTCACCCGGCCACCGCTTTTTCGCGGCGGCGGCGATCCGCACAATGGCGTCACTCGC
GCTGCACTCGGCGCAAACTTACTCCACGGAGCCCGACATGACGACCATTCTCCAGATCAATTCCGCCGCCCGCTCGCAAG
GCGCGCAGTCCACGCTGCTCGCCGACGAACTGACGGCCAAGCTGCAACAGGGCAACCCCGGCGCGACCGTCAAGGTCCGC
AACCTGCTCGCCGACGCGCTGCCGCACCTCGATGACGCGGTGCTGGGCGCGTTCTTCACGCCGGCCGACCAGCGCAGCGC
CGAGCAGAATGCGATCGTCGCGAAAAGCGATGAGCTGGTCGACGAGCTGCGCTCGGCCGATGTGATCGTGATCGGCGCGC
CGATGTACAACTTCGGCGTGTCGTCGCAGTTGAAGGCGTACTTCGACTGGATCGCCCGCGCGGGCGTCACGTTCCGCTAC
ACGTCGGAAGGCCCGGAAGGGCTGATCAAGGGCAAGAAGGCGTACGTGGTGTCGGCGCGCGGCGGCAAGCACGTCGGCAT
GCCGACCGACAGCCAGACGCCGTTCCTGAAGACGTTCCTCGGTTTCATCGGTCTGACCGACGTGACGTTCGTCTACGCGG
AAGGCCTCGCGCTCGGCCCGGATGCGGCGACGGAAGCGCTCGCGAGCGCACGCGAGGCGATCGCCGCGGTTTGA

Upstream 100 bases:

>100_bases
CGCTCAGCCGAAGCCCGCGGCCGCGCGCGCCCGTCGCGACGCGTGGCTCGGGCTGCCGGCGGCGCGCGGCGGTCGATAAT
CGCGCCGAACCGGGCGCGGA

Downstream 100 bases:

>100_bases
GCGTGATCGCTCGATAGCCGGAAAACCCTGAAAGAAAACGCCGCGGACCGGGAGGTGCGCGGCGTTTTTGCTTGCATCGT
CGGTGAACCGGTTGTCGAGC

Product: FMN-dependent NADH-azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MFRYCNNPSPGHRFFAAAAIRTMASLALHSAQTYSTEPDMTTILQINSAARSQGAQSTLLADELTAKLQQGNPGATVKVR
NLLADALPHLDDAVLGAFFTPADQRSAEQNAIVAKSDELVDELRSADVIVIGAPMYNFGVSSQLKAYFDWIARAGVTFRY
TSEGPEGLIKGKKAYVVSARGGKHVGMPTDSQTPFLKTFLGFIGLTDVTFVYAEGLALGPDAATEALASAREAIAAV

Sequences:

>Translated_237_residues
MFRYCNNPSPGHRFFAAAAIRTMASLALHSAQTYSTEPDMTTILQINSAARSQGAQSTLLADELTAKLQQGNPGATVKVR
NLLADALPHLDDAVLGAFFTPADQRSAEQNAIVAKSDELVDELRSADVIVIGAPMYNFGVSSQLKAYFDWIARAGVTFRY
TSEGPEGLIKGKKAYVVSARGGKHVGMPTDSQTPFLKTFLGFIGLTDVTFVYAEGLALGPDAATEALASAREAIAAV
>Mature_237_residues
MFRYCNNPSPGHRFFAAAAIRTMASLALHSAQTYSTEPDMTTILQINSAARSQGAQSTLLADELTAKLQQGNPGATVKVR
NLLADALPHLDDAVLGAFFTPADQRSAEQNAIVAKSDELVDELRSADVIVIGAPMYNFGVSSQLKAYFDWIARAGVTFRY
TSEGPEGLIKGKKAYVVSARGGKHVGMPTDSQTPFLKTFLGFIGLTDVTFVYAEGLALGPDAATEALASAREAIAAV

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=199, Percent_Identity=48.7437185929648, Blast_Score=191, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 25176; Mature: 25176

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRYCNNPSPGHRFFAAAAIRTMASLALHSAQTYSTEPDMTTILQINSAARSQGAQSTLL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCHHHHH
ADELTAKLQQGNPGATVKVRNLLADALPHLDDAVLGAFFTPADQRSAEQNAIVAKSDELV
HHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCEEEECCHHHH
DELRSADVIVIGAPMYNFGVSSQLKAYFDWIARAGVTFRYTSEGPEGLIKGKKAYVVSAR
HHHHCCCEEEECCCHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCEEEEEECC
GGKHVGMPTDSQTPFLKTFLGFIGLTDVTFVYAEGLALGPDAATEALASAREAIAAV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFRYCNNPSPGHRFFAAAAIRTMASLALHSAQTYSTEPDMTTILQINSAARSQGAQSTLL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCHHHHH
ADELTAKLQQGNPGATVKVRNLLADALPHLDDAVLGAFFTPADQRSAEQNAIVAKSDELV
HHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCEEEECCHHHH
DELRSADVIVIGAPMYNFGVSSQLKAYFDWIARAGVTFRYTSEGPEGLIKGKKAYVVSAR
HHHHCCCEEEECCCHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCEEEEEECC
GGKHVGMPTDSQTPFLKTFLGFIGLTDVTFVYAEGLALGPDAATEALASAREAIAAV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA