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The map label for this gene is iciA [H]

Identifier: 126447011

GI number: 126447011

Start: 30396

End: 31289

Strand: Direct

Name: iciA [H]

Synonym: BMA10247_A0036

Alternate gene names: 126447011

Gene position: 30396-31289 (Clockwise)

Preceding gene: 126446970

Following gene: 126445650

Centisome position: 1.29

GC content: 69.46

Gene sequence:

>894_bases
ATGCTCGACTATGCACTGCTCGATGCGCTTGCCGAAGTGATCCGCCACGGCTCGTTCGAGCGGGCGGCGAAAGCGCTCAA
CGTGTCGCCTTCGGCGGTGTCGCAGCGCGTGAAGCTCTTGGAGGAGCGCGTGGGCAGCGTGCTCGTGAAGCGCGGGCAGC
CGTGCGTCGCGACGACCTCGGGCGCGCTGCTGTGCCGCCACACCGAGCGCGTGCGGATGCTGGAGGCCGAGCTGTCCGGG
CACATGCCCGCGTTCGCGGGCGCGCAAGCGGCGGCGTGGCCGACGCTGCGCGTGGCCGTCAACGACGACAGCGTCGCGAC
CTGGTTCGTCGACGCGGTTGGCCCGTTCTGCGCGCAAAGCGGCACGCTGCTCGATCTCGTGATCGACGATCAGGACTACA
CCGCGGAGCGGATTCGCGACGGCAGCGTGCAGGGCGCGGTGACGGCGCTCGCCGAGCCGATTCAGGGCTGCCGCTCGACG
CGCCTTGGCCGGATGCGTTATCACGCGGTGTGCTCGCCCGCGTTCCACGCGCGCTATTTCGGCAACGGCGTCAATCGCGA
CGCGCTGCGGCGCGCGCCATGCGTGATGTTCAATCCGAAGGACAGCCTGCAGGCGCGCTTCATCCGGCGCGCGACGCGCG
TCGATCTCGATCCGCCGCAGCACTGGATTCCGCACGTCGCCGGTTATCTGCGCGCGTGCGAGGCGGGGCTCGGCTGGGGC
ATGTGTCCGGAGCGGATGGTCGAGCGCCAGATCGCCGAGGGCGGGCTCATCGATTTGTCGGACGGAAGGAGCATCGACAT
CGAGCTCTACTGGCAGAGCTGGCGGCTGTCGATCGGCTGGCTGGACGAGTTCAGCGCGGCGCTCAAGGCGAGCGCCGCGC
AGTATCTCGACTGA

Upstream 100 bases:

>100_bases
GCGGCTTCCGGCGGTGGACTAACGGGGCAAGAGGCGCTAGTCTGCGATGATCGCTTGAGTAAGAAAAGCTAAATGATCTA
ATGCCGATTAAGGAAAACTG

Downstream 100 bases:

>100_bases
CCGGTTGAGGGGGGCGAGGCGAAAGGGGCGGGCGCCGCGCGCCCGGTTACAGCCGGTGCAGCCCGTCGAAATCGACGATG
CGGACCATCCGGCCCTGCAT

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDYALLDALAEVIRHGSFERAAKALNVSPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVRMLEAELSG
HMPAFAGAQAAAWPTLRVAVNDDSVATWFVDAVGPFCAQSGTLLDLVIDDQDYTAERIRDGSVQGAVTALAEPIQGCRST
RLGRMRYHAVCSPAFHARYFGNGVNRDALRRAPCVMFNPKDSLQARFIRRATRVDLDPPQHWIPHVAGYLRACEAGLGWG
MCPERMVERQIAEGGLIDLSDGRSIDIELYWQSWRLSIGWLDEFSAALKASAAQYLD

Sequences:

>Translated_297_residues
MLDYALLDALAEVIRHGSFERAAKALNVSPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVRMLEAELSG
HMPAFAGAQAAAWPTLRVAVNDDSVATWFVDAVGPFCAQSGTLLDLVIDDQDYTAERIRDGSVQGAVTALAEPIQGCRST
RLGRMRYHAVCSPAFHARYFGNGVNRDALRRAPCVMFNPKDSLQARFIRRATRVDLDPPQHWIPHVAGYLRACEAGLGWG
MCPERMVERQIAEGGLIDLSDGRSIDIELYWQSWRLSIGWLDEFSAALKASAAQYLD
>Mature_297_residues
MLDYALLDALAEVIRHGSFERAAKALNVSPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVRMLEAELSG
HMPAFAGAQAAAWPTLRVAVNDDSVATWFVDAVGPFCAQSGTLLDLVIDDQDYTAERIRDGSVQGAVTALAEPIQGCRST
RLGRMRYHAVCSPAFHARYFGNGVNRDALRRAPCVMFNPKDSLQARFIRRATRVDLDPPQHWIPHVAGYLRACEAGLGWG
MCPERMVERQIAEGGLIDLSDGRSIDIELYWQSWRLSIGWLDEFSAALKASAAQYLD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=276, Percent_Identity=36.9565217391304, Blast_Score=158, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32684; Mature: 32684

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYALLDALAEVIRHGSFERAAKALNVSPSAVSQRVKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVRMLEAELSGHMPAFAGAQAAAWPTLRVAVNDDSVATWFVDAVGPFCAQS
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
GTLLDLVIDDQDYTAERIRDGSVQGAVTALAEPIQGCRSTRLGRMRYHAVCSPAFHARYF
CCEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHH
GNGVNRDALRRAPCVMFNPKDSLQARFIRRATRVDLDPPQHWIPHVAGYLRACEAGLGWG
CCCCCHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
MCPERMVERQIAEGGLIDLSDGRSIDIELYWQSWRLSIGWLDEFSAALKASAAQYLD
CCHHHHHHHHHHCCCCEECCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDYALLDALAEVIRHGSFERAAKALNVSPSAVSQRVKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVRMLEAELSGHMPAFAGAQAAAWPTLRVAVNDDSVATWFVDAVGPFCAQS
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
GTLLDLVIDDQDYTAERIRDGSVQGAVTALAEPIQGCRSTRLGRMRYHAVCSPAFHARYF
CCEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHH
GNGVNRDALRRAPCVMFNPKDSLQARFIRRATRVDLDPPQHWIPHVAGYLRACEAGLGWG
CCCCCHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
MCPERMVERQIAEGGLIDLSDGRSIDIELYWQSWRLSIGWLDEFSAALKASAAQYLD
CCHHHHHHHHHHCCCCEECCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA