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The map label for this gene is leuC [H]

Identifier: 126447007

GI number: 126447007

Start: 466804

End: 468213

Strand: Direct

Name: leuC [H]

Synonym: BMA10247_A0520

Alternate gene names: 126447007

Gene position: 466804-468213 (Clockwise)

Preceding gene: 126446474

Following gene: 126447682

Centisome position: 19.84

GC content: 67.45

Gene sequence:

>1410_bases
ATGGCACAGACGCTCTACGACAAATTGTGGAATTCGCACGTCGTCCACACCGAAGAGGACGGCACCGCACTGCTCTATAT
CGATCGCCAACTGCTGCATGAAGTCACGAGCCCGCAGGCGTTCGAAGGGCTGAAGCTCGCGCAGCGCCCGGTGTGGCGGA
TCAGCGCGAACCTCGCGGTGTCCGACCACAACGTGCCGACCACCGACCGCAGCCACGGCATCGCCGATCCGGTGTCGAAG
CTGCAGGTCGACACGCTCGACGCGAACTGCGACGCATATGGCATCACGCAATTCAAGATGAACGACGTGCGCCAGGGCAT
CGTCCATATCATCGGCCCGGAGCAGGGCGCGACGCTGCCCGGCATGACGATCGTCTGCGGCGATTCGCACACGTCGACGC
ACGGCGCGTTCGGCGCGCTCGCGCACGGCATCGGCACGTCGGAAGTCGAGCACGTGCTCGCGACGCAGACGCTCCTTCAG
AAGAAGAGCAAGAACATGCTCGTGAAGGTCGAGGGCCAACTGCCGCGCGGCTGCACCGCGAAGGACATCGTGCTCGCGAT
CATCGGCCAGATCGGCACCGCGGGCGGCACCGGCTACGCGATCGAATTCGGCGGCTCGACGATCCGCGCGCTCACGATGG
AAGGCCGGATGACGGTCTGCAACATGGCGATCGAGGCGGGCGCGCGCGCCGGCATGGTTGCCGTCGACGACACGACGGTC
GAATACCTGAAGGGCCGCCCGTTTGTGCCGACGGGCGCCGAATGGGATCAGGCGGTCGAATACTGGAAGACGTTCAGGTC
CGACGAGGGCGCGCAGTTCGACCGCGTCGTCGAGCTGGACGCGGCGCAGATCGTGCCGCAGGTCACGTGGGGCACGTCGC
CCGAGATGGTCACGTCGATCGACGGCCGCGTGCCCGATCCCGAGCGCGAGAAGGACCCGGTCAAGCGCGACGCGATGGAG
CGCGCGCTCGCATACATGGCGCTCGCGCCGAACACGCCGATCGAGGCGATCAAGGTCGACAAGATCTTCATCGGCTCGTG
CACGAACGCGCGGATCGAGGACATCCGCGCGGCCGCGTACGTCGTGAAGAAGCTCAATCGCCGCGTCGCGCCGAACGTGC
GGCTCGCGATGGTCGTGCCGGGCTCGGGCCTCGTGAAGGCGCAGGCCGAGCGCGAGGGGCTCGACAAGGTGTTCACCGAG
GCGGGCTTCGAATGGCGCGAGCCGGGCTGCTCGATGTGTCTCGCGATGAACGCCGACCGGCTCGAGCCGGGCGAGCGCTG
CGCGTCGACGTCGAACCGCAATTTCGAAGGGCGCCAGGGCCAGGGCGGCCGCACGCATCTCGTCAGCCCGGCGATGGCCG
CGGCGGCGGCGATCGAAGGCCACTTCGTCGATATTCGCCGGCTGGGGTGA

Upstream 100 bases:

>100_bases
TATGGGTTTTTTCGTTTCCCTGGTACAAAAGGGCGGGCTTCGCGTGGCATAATCGTCTGCACCCGAACGCATGTTCGCAG
TCAAACGACCCCGCATACCC

Downstream 100 bases:

>100_bases
GCGTGAACGCATCGGTTTTCCGGCGCATCGCCGCCGCGTTCGCGCTGGCGGGCTGCGTGCTCGGCTTGGCCGGCTGCAAC
ACGGTCGCCGGCGTGGGCGA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKLAQRPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDAYGITQFKMNDVRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQ
KKSKNMLVKVEGQLPRGCTAKDIVLAIIGQIGTAGGTGYAIEFGGSTIRALTMEGRMTVCNMAIEAGARAGMVAVDDTTV
EYLKGRPFVPTGAEWDQAVEYWKTFRSDEGAQFDRVVELDAAQIVPQVTWGTSPEMVTSIDGRVPDPEREKDPVKRDAME
RALAYMALAPNTPIEAIKVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVAPNVRLAMVVPGSGLVKAQAEREGLDKVFTE
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAIEGHFVDIRRLG

Sequences:

>Translated_469_residues
MAQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKLAQRPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDAYGITQFKMNDVRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQ
KKSKNMLVKVEGQLPRGCTAKDIVLAIIGQIGTAGGTGYAIEFGGSTIRALTMEGRMTVCNMAIEAGARAGMVAVDDTTV
EYLKGRPFVPTGAEWDQAVEYWKTFRSDEGAQFDRVVELDAAQIVPQVTWGTSPEMVTSIDGRVPDPEREKDPVKRDAME
RALAYMALAPNTPIEAIKVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVAPNVRLAMVVPGSGLVKAQAEREGLDKVFTE
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAIEGHFVDIRRLG
>Mature_468_residues
AQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKLAQRPVWRISANLAVSDHNVPTTDRSHGIADPVSKL
QVDTLDANCDAYGITQFKMNDVRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQK
KSKNMLVKVEGQLPRGCTAKDIVLAIIGQIGTAGGTGYAIEFGGSTIRALTMEGRMTVCNMAIEAGARAGMVAVDDTTVE
YLKGRPFVPTGAEWDQAVEYWKTFRSDEGAQFDRVVELDAAQIVPQVTWGTSPEMVTSIDGRVPDPEREKDPVKRDAMER
ALAYMALAPNTPIEAIKVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVAPNVRLAMVVPGSGLVKAQAEREGLDKVFTEA
GFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAIEGHFVDIRRLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=362, Percent_Identity=26.7955801104972, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8659555, Length=410, Percent_Identity=26.8292682926829, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI41352693, Length=377, Percent_Identity=25.7294429708223, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=63.2478632478632, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1787531, Length=373, Percent_Identity=26.2734584450402, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=377, Percent_Identity=28.1167108753316, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=377, Percent_Identity=28.1167108753316, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=302, Percent_Identity=27.1523178807947, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=407, Percent_Identity=26.5356265356265, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=63.9830508474576, Blast_Score=621, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6323335, Length=363, Percent_Identity=31.129476584022, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320440, Length=350, Percent_Identity=29.4285714285714, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=387, Percent_Identity=28.9405684754522, Blast_Score=136, Evalue=8e-33,
Organism=Drosophila melanogaster, GI281365315, Length=400, Percent_Identity=26.75, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=400, Percent_Identity=26.75, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=400, Percent_Identity=26.75, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=26.6247379454927, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17137564, Length=391, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645686, Length=377, Percent_Identity=26.2599469496021, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50775; Mature: 50644

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKLAQRPVWRISANLAV
CCHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHHCHHHHCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDAYGITQFKMNDVRQGIVHIIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEEEEHHHHHCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGQLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
KDIVLAIIGQIGTAGGTGYAIEFGGSTIRALTMEGRMTVCNMAIEAGARAGMVAVDDTTV
HHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCEEEEHHHHHCCCCCCEEEECCCHH
EYLKGRPFVPTGAEWDQAVEYWKTFRSDEGAQFDRVVELDAAQIVPQVTWGTSPEMVTSI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEECCHHHHCEEEECCCCCCCEEEC
DGRVPDPEREKDPVKRDAMERALAYMALAPNTPIEAIKVDKIFIGSCTNARIEDIRAAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH
VVKKLNRRVAPNVRLAMVVPGSGLVKAQAEREGLDKVFTEAGFEWREPGCSMCLAMNADR
HHHHHHHHCCCCEEEEEEECCCCCEECHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAIEGHFVDIRRLG
CCCHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
AQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKLAQRPVWRISANLAV
CHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHHCHHHHCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDAYGITQFKMNDVRQGIVHIIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEEEEHHHHHCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGQLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
KDIVLAIIGQIGTAGGTGYAIEFGGSTIRALTMEGRMTVCNMAIEAGARAGMVAVDDTTV
HHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCEEEEHHHHHCCCCCCEEEECCCHH
EYLKGRPFVPTGAEWDQAVEYWKTFRSDEGAQFDRVVELDAAQIVPQVTWGTSPEMVTSI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEECCHHHHCEEEECCCCCCCEEEC
DGRVPDPEREKDPVKRDAMERALAYMALAPNTPIEAIKVDKIFIGSCTNARIEDIRAAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH
VVKKLNRRVAPNVRLAMVVPGSGLVKAQAEREGLDKVFTEAGFEWREPGCSMCLAMNADR
HHHHHHHHCCCCEEEEEEECCCCCEECHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAIEGHFVDIRRLG
CCCHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA