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The map label for this gene is dmlR [H]

Identifier: 126446886

GI number: 126446886

Start: 12175

End: 13092

Strand: Direct

Name: dmlR [H]

Synonym: BMA10247_A0015

Alternate gene names: 126446886

Gene position: 12175-13092 (Clockwise)

Preceding gene: 126447205

Following gene: 126446127

Centisome position: 0.52

GC content: 67.21

Gene sequence:

>918_bases
GTGAACAATTCGCCGAACCTCGACGATCTGCGCGTGTTCAGCGTCGTCGTGCGGCTCGCGAGCTTCAGCGCGGCCGCCGA
ACAGCTCGCGGTGTCGCCCGCGTACGTCAGCAAGCGCGTGGCGCTGCTCGAAAAGCAACTCGGCACGCGGCTCCTGCATC
GCTCGACGCGCCGCGTCGCGGTGACGGAGGCCGGCGAGCGGGTTTATGCGTGGACTGAAAAGATCCTCGACGATGTCGAT
CATCTCGTCGAAGACGTATCGACGACCCGCAGCGTGCCGCGCGGCACGCTGCGGATTTCGAGCAGCTTCGGCTTCGGCCG
GCACGTGCTCGCGCCCGCGCTGCTCGGCTTCAACGAACGCTATCCGCAACTGAACGTGCGGCTCGATCTGTTCGACCGGC
TCGTCGACGTCGCGGGCGAAGGCTTCGATCTCGACATCCGGATCGGCGACGAGATCGCCGATCATCTGATCGCGAAGCGG
CTCGCGACGAACTACCGGGTGCTGTGCGCATCGCCAGGCTATCTCGCGCGATACGGCACGCCGCGCCAGCTCGCGGATCT
CGGCGCGCATCAGTGCCTGGCGATCAAGGAGCGCGATCATCCGTTCGGCGTATGGCGGCTCACGGTGCGCGGCGAGACGT
CGACGGTGAAGGTGGGCGGCGCGCTGTCGACGAACCACGGCGAGGTTGCCGTGCAGTGGGCGCTCGCCGGACGTGGAATC
GTGCTGCGCTCGATCTGGGAAGCGGGGCCGCTGCTCGCGAGCGGCGAGCTCAGGCGCGTGTTGCCCGAGGCGAGCCAGCC
GGCGAACGTGTGGGCCGTTTATCCCGCACGGCTTGCGGCTTCGGCGAAGGTACGCGTGTGTGTCGATTTCCTCGTGGACG
CGTTCGCGCATCTGAATGAACGCGCGAACGGCGGATAA

Upstream 100 bases:

>100_bases
AAGCGATCGCGCGGAGCTTGCGCGATCGCCGATTCATGCATTCTATTTATTCCTGGCGGGATTAAAATCGGCCATTCGGT
TAATCGACTTTCCACGCATC

Downstream 100 bases:

>100_bases
TCGTCTGGTGAACGGTGAACGGTGAACGGTGAACGGCGCGGGTCGACACGTAACGGGTAGTAGCGAGTCGACGATGATGA
CTGATCGATGGTTGATCCTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNNSPNLDDLRVFSVVVRLASFSAAAEQLAVSPAYVSKRVALLEKQLGTRLLHRSTRRVAVTEAGERVYAWTEKILDDVD
HLVEDVSTTRSVPRGTLRISSSFGFGRHVLAPALLGFNERYPQLNVRLDLFDRLVDVAGEGFDLDIRIGDEIADHLIAKR
LATNYRVLCASPGYLARYGTPRQLADLGAHQCLAIKERDHPFGVWRLTVRGETSTVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLLASGELRRVLPEASQPANVWAVYPARLAASAKVRVCVDFLVDAFAHLNERANGG

Sequences:

>Translated_305_residues
MNNSPNLDDLRVFSVVVRLASFSAAAEQLAVSPAYVSKRVALLEKQLGTRLLHRSTRRVAVTEAGERVYAWTEKILDDVD
HLVEDVSTTRSVPRGTLRISSSFGFGRHVLAPALLGFNERYPQLNVRLDLFDRLVDVAGEGFDLDIRIGDEIADHLIAKR
LATNYRVLCASPGYLARYGTPRQLADLGAHQCLAIKERDHPFGVWRLTVRGETSTVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLLASGELRRVLPEASQPANVWAVYPARLAASAKVRVCVDFLVDAFAHLNERANGG
>Mature_305_residues
MNNSPNLDDLRVFSVVVRLASFSAAAEQLAVSPAYVSKRVALLEKQLGTRLLHRSTRRVAVTEAGERVYAWTEKILDDVD
HLVEDVSTTRSVPRGTLRISSSFGFGRHVLAPALLGFNERYPQLNVRLDLFDRLVDVAGEGFDLDIRIGDEIADHLIAKR
LATNYRVLCASPGYLARYGTPRQLADLGAHQCLAIKERDHPFGVWRLTVRGETSTVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLLASGELRRVLPEASQPANVWAVYPARLAASAKVRVCVDFLVDAFAHLNERANGG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=60.1351351351351, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=49.1408934707904, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=34.9480968858132, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=33.1081081081081, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=30.3448275862069, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=34.7222222222222, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=29.020979020979, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=27.6923076923077, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789173, Length=239, Percent_Identity=28.4518828451883, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786448, Length=251, Percent_Identity=27.4900398406374, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788748, Length=133, Percent_Identity=33.0827067669173, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33411; Mature: 33411

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSPNLDDLRVFSVVVRLASFSAAAEQLAVSPAYVSKRVALLEKQLGTRLLHRSTRRVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VTEAGERVYAWTEKILDDVDHLVEDVSTTRSVPRGTLRISSSFGFGRHVLAPALLGFNER
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
YPQLNVRLDLFDRLVDVAGEGFDLDIRIGDEIADHLIAKRLATNYRVLCASPGYLARYGT
CCEEEEEHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEEECCCCHHHCCC
PRQLADLGAHQCLAIKERDHPFGVWRLTVRGETSTVKVGGALSTNHGEVAVQWALAGRGI
CHHHHHCCCHHEEEEECCCCCCEEEEEEEECCCCEEEECCEEECCCCCEEEEEEECCCCH
VLRSIWEAGPLLASGELRRVLPEASQPANVWAVYPARLAASAKVRVCVDFLVDAFAHLNE
HHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHH
RANGG
CCCCC
>Mature Secondary Structure
MNNSPNLDDLRVFSVVVRLASFSAAAEQLAVSPAYVSKRVALLEKQLGTRLLHRSTRRVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VTEAGERVYAWTEKILDDVDHLVEDVSTTRSVPRGTLRISSSFGFGRHVLAPALLGFNER
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
YPQLNVRLDLFDRLVDVAGEGFDLDIRIGDEIADHLIAKRLATNYRVLCASPGYLARYGT
CCEEEEEHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEEECCCCHHHCCC
PRQLADLGAHQCLAIKERDHPFGVWRLTVRGETSTVKVGGALSTNHGEVAVQWALAGRGI
CHHHHHCCCHHEEEEECCCCCCEEEEEEEECCCCEEEECCEEECCCCCEEEEEEECCCCH
VLRSIWEAGPLLASGELRRVLPEASQPANVWAVYPARLAASAKVRVCVDFLVDAFAHLNE
HHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHH
RANGG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]