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The map label for this gene is iaaA [H]

Identifier: 126446834

GI number: 126446834

Start: 1329115

End: 1330161

Strand: Reverse

Name: iaaA [H]

Synonym: BMA10247_A1362

Alternate gene names: 126446834

Gene position: 1330161-1329115 (Counterclockwise)

Preceding gene: 126446471

Following gene: 126447782

Centisome position: 56.54

GC content: 73.54

Gene sequence:

>1047_bases
ATGACTTCACCCGCAATCGTTGCGATTCACGGCGGCGCAGGCACGATCCTGCGCGAGGCGATGACGGCCGACGCCGAAGC
GCAGTACCGTGCGGAGCTCGCCGCGATCCTCGCCGCCGCGCAGAAGGTGCTCGCCGAAGGCGGCAGCGCGCTTGACGCGG
TCAGCGTCGCCGTGCGCATGCTCGAGGATTGCCCGCTTTTCAACGCGGGGCGCGGCGCCGTCTACACGGCGGACGGCACG
CACGAGCTCGACGCCGCGATCATGGACGGCGCGACGCTCGCGGCGGGCGCGATCTGCTGCGCGACGCGCGTGCGCAACCC
GGTGCTCGCCGCGCGCCGCGTGCTCGAAGCGAGCGAGCATGTGATGTTCGCGGGCGCGGGCGCCGACGCGTTCGCGGCTG
CGCAGGGGCTCGAGCTCGCGCCGCCGGGCTACTTCGACACCGAGCCGCGCCGCGCGCAATGGCTGAAGGCGCGACGCGCG
GCGGGCACGATGCTCGATCACGACGCGGCGGCGTTCGTGTTCGGCCGGGGGGGCGGCGATGGCGGCGGTAATGGCGGCGG
TAATGGCGGCGGCCCCGGCGCGCGCGCCGCGTGCGCGCCCGAGCCGCTCGATCCGGACCGCAAGCACGGCACGGTCGGCG
CGGTCGCGCGCGATCTGCACGGCCACCTCGCGGCGGCCACGTCGACGGGCGGGATCACGAACAAGCAGCCGGGGCGCGTC
GGCGATACGCCGATCATCGGCGCGGGCTGCTATGCGAACGACGCGACGTGCGCGGTATCGGCGACAGGCACGGGCGAGAT
GTTCATCCGCCTCGCGACCGCGTACGACGTGTCCGCGCAGATCGAATACCGCGGCGCGTCGCTTGCCGGCGCCGCGTACG
ACGTCGTGATGAACAAGCTGCCGAGCATCGCCGGCCGCGGCGGCATCATCGCGGTCGACGCGCGCGGCAACCTCGCGATG
CCGTTCAACACCGAAGGGATGTACCGCGGCTACGCGCGCGTCGGCGAGGCGCCCGTGACCGCGATCTATCGCGAGGACGC
GGCGTGA

Upstream 100 bases:

>100_bases
CGGCCGCCGAAGTGACCGAATTCATGCTGCCGTGGCTCGAGGCGCGGGGCGCATGCGCCGCGCCGCCGCGCCCGCCGCGC
CGTTCCAGGAAAACCGACCG

Downstream 100 bases:

>100_bases
CGCCGCGAACGAGCGCAGACAGCACCGACAGGAGAAACCGATGAGCGCGAGCCGAGCCGCTCCGAGCCTGCCCGACGCGC
GCGTGCTCGCGGTCGACGGG

Product: asparaginase family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTSPAIVAIHGGAGTILREAMTADAEAQYRAELAAILAAAQKVLAEGGSALDAVSVAVRMLEDCPLFNAGRGAVYTADGT
HELDAAIMDGATLAAGAICCATRVRNPVLAARRVLEASEHVMFAGAGADAFAAAQGLELAPPGYFDTEPRRAQWLKARRA
AGTMLDHDAAAFVFGRGGGDGGGNGGGNGGGPGARAACAPEPLDPDRKHGTVGAVARDLHGHLAAATSTGGITNKQPGRV
GDTPIIGAGCYANDATCAVSATGTGEMFIRLATAYDVSAQIEYRGASLAGAAYDVVMNKLPSIAGRGGIIAVDARGNLAM
PFNTEGMYRGYARVGEAPVTAIYREDAA

Sequences:

>Translated_348_residues
MTSPAIVAIHGGAGTILREAMTADAEAQYRAELAAILAAAQKVLAEGGSALDAVSVAVRMLEDCPLFNAGRGAVYTADGT
HELDAAIMDGATLAAGAICCATRVRNPVLAARRVLEASEHVMFAGAGADAFAAAQGLELAPPGYFDTEPRRAQWLKARRA
AGTMLDHDAAAFVFGRGGGDGGGNGGGNGGGPGARAACAPEPLDPDRKHGTVGAVARDLHGHLAAATSTGGITNKQPGRV
GDTPIIGAGCYANDATCAVSATGTGEMFIRLATAYDVSAQIEYRGASLAGAAYDVVMNKLPSIAGRGGIIAVDARGNLAM
PFNTEGMYRGYARVGEAPVTAIYREDAA
>Mature_347_residues
TSPAIVAIHGGAGTILREAMTADAEAQYRAELAAILAAAQKVLAEGGSALDAVSVAVRMLEDCPLFNAGRGAVYTADGTH
ELDAAIMDGATLAAGAICCATRVRNPVLAARRVLEASEHVMFAGAGADAFAAAQGLELAPPGYFDTEPRRAQWLKARRAA
GTMLDHDAAAFVFGRGGGDGGGNGGGNGGGPGARAACAPEPLDPDRKHGTVGAVARDLHGHLAAATSTGGITNKQPGRVG
DTPIIGAGCYANDATCAVSATGTGEMFIRLATAYDVSAQIEYRGASLAGAAYDVVMNKLPSIAGRGGIIAVDARGNLAMP
FNTEGMYRGYARVGEAPVTAIYREDAA

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=335, Percent_Identity=34.6268656716418, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI145275200, Length=335, Percent_Identity=34.6268656716418, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI285002251, Length=264, Percent_Identity=34.8484848484849, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787050, Length=341, Percent_Identity=53.9589442815249, Blast_Score=348, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17569239, Length=266, Percent_Identity=24.4360902255639, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI18921183, Length=341, Percent_Identity=35.4838709677419, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21357893, Length=290, Percent_Identity=31.3793103448276, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI221330102, Length=258, Percent_Identity=29.4573643410853, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652135, Length=258, Percent_Identity=29.4573643410853, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 35115; Mature: 34984

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPAIVAIHGGAGTILREAMTADAEAQYRAELAAILAAAQKVLAEGGSALDAVSVAVRM
CCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEDCPLFNAGRGAVYTADGTHELDAAIMDGATLAAGAICCATRVRNPVLAARRVLEASEH
HHHCCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VMFAGAGADAFAAAQGLELAPPGYFDTEPRRAQWLKARRAAGTMLDHDAAAFVFGRGGGD
EEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
GGGNGGGNGGGPGARAACAPEPLDPDRKHGTVGAVARDLHGHLAAATSTGGITNKQPGRV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
GDTPIIGAGCYANDATCAVSATGTGEMFIRLATAYDVSAQIEYRGASLAGAAYDVVMNKL
CCCCEEECCCCCCCCEEEEEECCCCEEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHH
PSIAGRGGIIAVDARGNLAMPFNTEGMYRGYARVGEAPVTAIYREDAA
HHHCCCCCEEEEECCCCEECCCCCCCHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure 
TSPAIVAIHGGAGTILREAMTADAEAQYRAELAAILAAAQKVLAEGGSALDAVSVAVRM
CCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEDCPLFNAGRGAVYTADGTHELDAAIMDGATLAAGAICCATRVRNPVLAARRVLEASEH
HHHCCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VMFAGAGADAFAAAQGLELAPPGYFDTEPRRAQWLKARRAAGTMLDHDAAAFVFGRGGGD
EEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
GGGNGGGNGGGPGARAACAPEPLDPDRKHGTVGAVARDLHGHLAAATSTGGITNKQPGRV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
GDTPIIGAGCYANDATCAVSATGTGEMFIRLATAYDVSAQIEYRGASLAGAAYDVVMNKL
CCCCEEECCCCCCCCEEEEEECCCCEEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHH
PSIAGRGGIIAVDARGNLAMPFNTEGMYRGYARVGEAPVTAIYREDAA
HHHCCCCCEEEEECCCCEECCCCCCCHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]