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The map label for this gene is benM [H]

Identifier: 126446604

GI number: 126446604

Start: 183896

End: 184816

Strand: Direct

Name: benM [H]

Synonym: BMA10247_A0216

Alternate gene names: 126446604

Gene position: 183896-184816 (Clockwise)

Preceding gene: 126445959

Following gene: 126447880

Centisome position: 7.82

GC content: 66.12

Gene sequence:

>921_bases
ATGAAGATGGAACTGCGGCATCTACGCTACTTCGTCGCGGTCGCGGAGGAGCAGAACTTCACGCGCGCGGCCGAGCGCCT
GCACATCGCGCAGCCGCCGTTGAGCCGCCAGATCCAGCAGCTCGAGGAGGCGCTCGGCGTGACGCTGTTCGAGCGCCATG
CGCGGCCGCTGAAGATGACCGACGCGGGGCGCTTCTTCTATTCGCACGCGGTGCAATTGCTCGCGCAGACAGCCGAGCTC
GAAGCGATGACGAAGCGCGTCGGCAAGATCGAGCGCGCGCTGTCGGTGGGCTTTGTCGGCTCGACGCTGTACGGGATGCT
GCCGCGCATCATCCGGCGCTTTCGCGCCGAGCACGGCGAAGTCGAGCTGAGCCTGCACGAGATGTCGACGATGGATCAGA
TCCAGGCGCTCAAGGACGGGCGCATCGACGTCGGCTTCGGGCGCATTCGTCATGAGGATGCGCGCGTGCGGCGCGTCGTG
CTGCGCGAGGAGCGGATGATCGTCGCGCTGCCGGACGGGCATCCGCTCGAGGCGGCCAAGCCGGTGCTGTCGCTGCACGA
CCTCGTCAACGAGACGCTGATCATCTTTCCGAAGTCGCCGCGGCCGAGCTACGCGGACCAGGTGCTCGCCGCGTTTCACG
ACCGCGCGCTGAAGCCGCGCCGGATCTACGAGACGCGCGAGCTGCAGATCGCGCTCGGGCTCGTCGCGGCGGGCGAGGGC
GTGTCCGTCGTGCCGCACAGCGTGTACGGGCTCAAGCGCGACGACGTCGGCTACAAGGAGCTCGACGATCCGAACCTCGT
GTCGCCGATCATCATGAGCACGCGGATGCTCGACGAATCGGGGGACATTCGCGCGATGCTCGAGTTGATTTATCGTTTGT
ACGACGAATTCAAGATGACGTATCTGCCGCCGCACGGATGA

Upstream 100 bases:

>100_bases
CAGGTATAGTGCTCGACGACAGCGCGGTCAGATTGAGGATAGTTGCCGGCAAAACGGTGCGTCCAACACTGATTGAGTAT
CGGATCGATATCCTCGAGGT

Downstream 100 bases:

>100_bases
GCGGGAGGCGGGAAGCGCGATGTCGCGGCGCGTGGCGGGGAGCGCCGGCGTGCCGATGGCGCACGTTTCGCGCGCACGGG
GTGGGCGCGCGAGCGACGCC

Product: ben operon transcriptional regulator BenM

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKMELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVTLFERHARPLKMTDAGRFFYSHAVQLLAQTAEL
EAMTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVV
LREERMIVALPDGHPLEAAKPVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEG
VSVVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESGDIRAMLELIYRLYDEFKMTYLPPHG

Sequences:

>Translated_306_residues
MKMELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVTLFERHARPLKMTDAGRFFYSHAVQLLAQTAEL
EAMTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVV
LREERMIVALPDGHPLEAAKPVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEG
VSVVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESGDIRAMLELIYRLYDEFKMTYLPPHG
>Mature_306_residues
MKMELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVTLFERHARPLKMTDAGRFFYSHAVQLLAQTAEL
EAMTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGEVELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVV
LREERMIVALPDGHPLEAAKPVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEG
VSVVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESGDIRAMLELIYRLYDEFKMTYLPPHG

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=274, Percent_Identity=33.9416058394161, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788887, Length=264, Percent_Identity=32.5757575757576, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI2367136, Length=266, Percent_Identity=30.8270676691729, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=29.5081967213115, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693105, Length=277, Percent_Identity=28.5198555956679, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787601, Length=251, Percent_Identity=27.8884462151394, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI157672245, Length=244, Percent_Identity=28.6885245901639, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081904, Length=266, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=29.8804780876494, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=28.9156626506024, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789204, Length=243, Percent_Identity=27.9835390946502, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790208, Length=146, Percent_Identity=35.6164383561644, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788481, Length=146, Percent_Identity=30.8219178082192, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082132, Length=245, Percent_Identity=27.7551020408163, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=26.4, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=28.6290322580645, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786401, Length=180, Percent_Identity=32.7777777777778, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790783, Length=113, Percent_Identity=31.858407079646, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=147, Percent_Identity=26.530612244898, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=139, Percent_Identity=33.8129496402878, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789173, Length=280, Percent_Identity=25.7142857142857, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34913; Mature: 34913

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVTLFERHARPLKMT
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCEEC
DAGRFFYSHAVQLLAQTAELEAMTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVVLREERMIVALPDGHPLEAAK
EEEEHHHHHHHHHHHHHHCCCEEECCCHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHH
PVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEG
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEECCCC
VSVVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESGDIRAMLELIYRLYDEFKMT
CEECCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEE
YLPPHG
ECCCCC
>Mature Secondary Structure
MKMELRHLRYFVAVAEEQNFTRAAERLHIAQPPLSRQIQQLEEALGVTLFERHARPLKMT
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCEEC
DAGRFFYSHAVQLLAQTAELEAMTKRVGKIERALSVGFVGSTLYGMLPRIIRRFRAEHGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VELSLHEMSTMDQIQALKDGRIDVGFGRIRHEDARVRRVVLREERMIVALPDGHPLEAAK
EEEEHHHHHHHHHHHHHHCCCEEECCCHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHH
PVLSLHDLVNETLIIFPKSPRPSYADQVLAAFHDRALKPRRIYETRELQIALGLVAAGEG
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEECCCC
VSVVPHSVYGLKRDDVGYKELDDPNLVSPIIMSTRMLDESGDIRAMLELIYRLYDEFKMT
CEECCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEE
YLPPHG
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]