Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 126446548

GI number: 126446548

Start: 2338208

End: 2339143

Strand: Reverse

Name: pleD [H]

Synonym: BMA10247_A2399

Alternate gene names: 126446548

Gene position: 2339143-2338208 (Counterclockwise)

Preceding gene: 126446418

Following gene: 126447279

Centisome position: 99.42

GC content: 66.67

Gene sequence:

>936_bases
ATGAACCTGCTTTCCTCGCTGAGCGATCTCGTCATCGAACGGGTCAGCTTCGGTATTTTCGTGCTCGACCGCGAGATGAG
GGTACTCATGTGGAACCGGTTCATGCAGGACCATAGCGGCGTCGCGGCGGCCGACGTGATCGGCCGCAACCTGTTCGACA
GCTTTCCCGAGCTGCCGCGCGCGTGGCTGTCGCGCAAGATCGAGAGCGTGTTCCAGCTCGGCAGCTTCGCGTTCAGCTCG
TGGGAGCAGCGCCCGTATCTGTTCCGCTTCGATCACGATCGGCCGATCACGGGCGGCGTCGACTTCATGCAGCAGGACTG
CACGTTCGTGCCGATCGCGCGCGCGCGCGAAGTGGAGGCGGTCTGCGTGACCGTCTCCGATGTCACGCACGTGAGCGTGA
TGCAGCGCGAGCGCGAGGAGGCCGTCGCGAAGCTGCAGGAATACGCGAACCGCGACGGCCTGACGGGCATCGCGAACCGG
CGCTTCTTCGAGGCGCGGCTGCGCGACGAATTCTCGCGCTGGCAGCGCTACGGCGGCAATCTGTCGGTGCTGCTGTTCGA
TCTCGACCATTTCAAGACGATCAACGATCGCTTCGGCCACGTCGTCGGGGACAACGTGCTGCGCGTGATGGCGCAGCGTG
TCGCGGACGTCGTGCGCTCGCAGGACACGTTCGGCCGCTTCGGCGGCGAGGAATTCGCGCTGCTGCTGCCGTGCACGTCG
CTCGACGAGGCGATGCTCGTTGCCGAGAAGATCCGGCAGACGATCGGCAGCACCCCGATCGAAGCGGAGGGCGTGCGGGT
GCCGGTGACGGCGAGCGTCGGCGGCGCGGCCGCGCACGCCGGCGCGCTCACGCACGAGACGCTCGTCAACGAGGCCGACG
CGGCGCTTTATCGCGCGAAACGGCAGGGACGGAACTGCTCGGTTGCGTTCGGTTGA

Upstream 100 bases:

>100_bases
GCGTCGCGGACGACGGCGCGCGCACGCACTTCGTGATGCTGCTCGCCGAAGATTCGATTCGCCACATGAGCGACGCGCTC
GATTTGTTGCTGTCCAGCTT

Downstream 100 bases:

>100_bases
GGCAGTGCGGCCGGCAAGCCGGCCGCGCCGCGGCGGACCAACGGCGTCAGCCTCGCGCGATCGCGGCGAGCGTATCGGCT
ACCGCGCCCGCGTGCGCGAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNLLSSLSDLVIERVSFGIFVLDREMRVLMWNRFMQDHSGVAAADVIGRNLFDSFPELPRAWLSRKIESVFQLGSFAFSS
WEQRPYLFRFDHDRPITGGVDFMQQDCTFVPIARAREVEAVCVTVSDVTHVSVMQREREEAVAKLQEYANRDGLTGIANR
RFFEARLRDEFSRWQRYGGNLSVLLFDLDHFKTINDRFGHVVGDNVLRVMAQRVADVVRSQDTFGRFGGEEFALLLPCTS
LDEAMLVAEKIRQTIGSTPIEAEGVRVPVTASVGGAAAHAGALTHETLVNEADAALYRAKRQGRNCSVAFG

Sequences:

>Translated_311_residues
MNLLSSLSDLVIERVSFGIFVLDREMRVLMWNRFMQDHSGVAAADVIGRNLFDSFPELPRAWLSRKIESVFQLGSFAFSS
WEQRPYLFRFDHDRPITGGVDFMQQDCTFVPIARAREVEAVCVTVSDVTHVSVMQREREEAVAKLQEYANRDGLTGIANR
RFFEARLRDEFSRWQRYGGNLSVLLFDLDHFKTINDRFGHVVGDNVLRVMAQRVADVVRSQDTFGRFGGEEFALLLPCTS
LDEAMLVAEKIRQTIGSTPIEAEGVRVPVTASVGGAAAHAGALTHETLVNEADAALYRAKRQGRNCSVAFG
>Mature_311_residues
MNLLSSLSDLVIERVSFGIFVLDREMRVLMWNRFMQDHSGVAAADVIGRNLFDSFPELPRAWLSRKIESVFQLGSFAFSS
WEQRPYLFRFDHDRPITGGVDFMQQDCTFVPIARAREVEAVCVTVSDVTHVSVMQREREEAVAKLQEYANRDGLTGIANR
RFFEARLRDEFSRWQRYGGNLSVLLFDLDHFKTINDRFGHVVGDNVLRVMAQRVADVVRSQDTFGRFGGEEFALLLPCTS
LDEAMLVAEKIRQTIGSTPIEAEGVRVPVTASVGGAAAHAGALTHETLVNEADAALYRAKRQGRNCSVAFG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=179, Percent_Identity=39.6648044692737, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=35.1851851851852, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87081881, Length=185, Percent_Identity=37.8378378378378, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=40.6060606060606, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787262, Length=196, Percent_Identity=33.1632653061224, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=36.4197530864198, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787541, Length=306, Percent_Identity=25.8169934640523, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=32.5966850828729, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=196, Percent_Identity=29.5918367346939, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=166, Percent_Identity=30.1204819277108, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=173, Percent_Identity=33.5260115606936, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=35.7575757575758, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=112, Percent_Identity=27.6785714285714, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34943; Mature: 34943

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLSSLSDLVIERVSFGIFVLDREMRVLMWNRFMQDHSGVAAADVIGRNLFDSFPELPR
CCHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AWLSRKIESVFQLGSFAFSSWEQRPYLFRFDHDRPITGGVDFMQQDCTFVPIARAREVEA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCEEECHHCCCCEE
VCVTVSDVTHVSVMQREREEAVAKLQEYANRDGLTGIANRRFFEARLRDEFSRWQRYGGN
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LSVLLFDLDHFKTINDRFGHVVGDNVLRVMAQRVADVVRSQDTFGRFGGEEFALLLPCTS
EEEEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCC
LDEAMLVAEKIRQTIGSTPIEAEGVRVPVTASVGGAAAHAGALTHETLVNEADAALYRAK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQGRNCSVAFG
HCCCCCEEECC
>Mature Secondary Structure
MNLLSSLSDLVIERVSFGIFVLDREMRVLMWNRFMQDHSGVAAADVIGRNLFDSFPELPR
CCHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AWLSRKIESVFQLGSFAFSSWEQRPYLFRFDHDRPITGGVDFMQQDCTFVPIARAREVEA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCEEECHHCCCCEE
VCVTVSDVTHVSVMQREREEAVAKLQEYANRDGLTGIANRRFFEARLRDEFSRWQRYGGN
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LSVLLFDLDHFKTINDRFGHVVGDNVLRVMAQRVADVVRSQDTFGRFGGEEFALLLPCTS
EEEEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCC
LDEAMLVAEKIRQTIGSTPIEAEGVRVPVTASVGGAAAHAGALTHETLVNEADAALYRAK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQGRNCSVAFG
HCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA