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The map label for this gene is pfk [H]

Identifier: 126446308

GI number: 126446308

Start: 114905

End: 116254

Strand: Direct

Name: pfk [H]

Synonym: BMA10247_A0142

Alternate gene names: 126446308

Gene position: 114905-116254 (Clockwise)

Preceding gene: 126447910

Following gene: 126445615

Centisome position: 4.88

GC content: 72.3

Gene sequence:

>1350_bases
GTGCGCGGCGCTTGCGCGTCGCGCGCGATGTTCGATACTGGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGC
AAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAGCGGCAAG
CGGCAAGCGGCAAGCGGCAAGCGGCAAGCAGCAAGCAGCAAGCAGCAAGCAGCAAGCAGCAAGCAGCAAGCAGCAAGCAG
CAAGCAGCAAGCAGCAGTCGACCTGCGCCGGTGCTGATGGTTCGACGCCGAATCGAACGCAAGCATTGCGGCGCGTGCCC
GCCGCGCAGCACCCGCGAGCCGCGCACCCGAGCCAGCCGCTGCGTTGTCTTTCCGTCGCGAATTCCCACGTTGAAACCGA
ACGTCACGATGCCCGAAATCGTCACCCTCACGCCGAATCCCGCCATCGACGTCGCGACGTGCGTCGAGCGAGTCACCGAC
ACCCGCAAGCTGCGCTGCGGCCCCGCACGACGCGATCCGGGCGGCGGCGGAATCAATGTCGCCCGCGTGCTGACGCGCCT
CGGCGCCGATTGTTCGGCCGTCTACCTGGCGGGCGGCGGCACCGGCCTCGCGCTGCGCGGCCTGCTCGCCGACGAAGGCG
TGCGGGCGCATGGCATCGACATCGCCGGCGAGACGCGCGAAAACTTTTCGGTGCTCGAAACGTCGACGGGCCGCGAATTC
CGCTTCGTGTTGCCCGGGCCGGCGCTCGCGGCGCACGAATGGCCGCGCTGCGTCGAAGCGCTCGGGCGGCTCGCCGACGC
GTCGCGCTATCTGGTGATGAGCGGCAGCCTGCCGCCCGGCATGCCGGACGATTGCTACGCCCGGCTCGCGCGGCGCGCGA
GCGCGCGCGGCGTGCGCACGGTCGTCGATACGTCGGGACCGGCGCTCGCCGCCGCGCTCGATGCGGGCGTCTATCTGGTG
AAGCCGAGCCTCGGCGAGTTGCGCGCGCTGACCGGCCTGCCGCTCGAAGACGACGGCGCGCGCCTTGCCGCCGCGCGCGC
GATCGTCGCCGGCGGGCGCGCGCAGATCGTCGCGCTGACGCTCGGCGACGCGGGCGCGCTCGTCGTGTCGCGCGACGATG
CGGTGCGTCTGCCGGGCGTGAAGGTGGCGGTGCGCAGCGCGATCGGCGCGGGCGACAGCTTCGTCGCGGGGCTCGTCGCC
GCGCTCAATCGCGGCGCGAACGTCGCCGACGCGGCGCGCCATGCGCTCGCCGCCGCGTCGGCGTCGCTGCTGAGCACGGG
CACCGCGCTGGGCACGAAGGAAGACATCGCGCGGATCTATCGCGAACTGCCGGGGACGATCGACGCGTGA

Upstream 100 bases:

>100_bases
CGCTGATCGAGCGGCGCGCGGCTTGCGGCGCCGCATGGTCGGTGAAGGGCGTGCGCGAAGTGATCGATCATCTGAGCGTC
GCATAGGCGCGCGAGGCAAT

Downstream 100 bases:

>100_bases
CACGTGACGCGCCGGGCGCGCGCGCCGCCGGCCGCCATTCTCCCGCGCGCGACCTTTTGCAGTTGCGACCGCTTCGCGCT
CGCCCATCCAGCGCGCCTGC

Product: 6-phosphofructokinase

Products: ADP; D-Fructose 1,6-bisphosphate; H+ [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MRGACASRAMFDTGGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASSKQQAASSKQQAASSKQ
QAASSSRPAPVLMVRRRIERKHCGACPPRSTREPRTRASRCVVFPSRIPTLKPNVTMPEIVTLTPNPAIDVATCVERVTD
TRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRAHGIDIAGETRENFSVLETSTGREF
RFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARGVRTVVDTSGPALAAALDAGVYLV
KPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVRLPGVKVAVRSAIGAGDSFVAGLVA
ALNRGANVADAARHALAAASASLLSTGTALGTKEDIARIYRELPGTIDA

Sequences:

>Translated_449_residues
MRGACASRAMFDTGGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASSKQQAASSKQQAASSKQ
QAASSSRPAPVLMVRRRIERKHCGACPPRSTREPRTRASRCVVFPSRIPTLKPNVTMPEIVTLTPNPAIDVATCVERVTD
TRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRAHGIDIAGETRENFSVLETSTGREF
RFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARGVRTVVDTSGPALAAALDAGVYLV
KPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVRLPGVKVAVRSAIGAGDSFVAGLVA
ALNRGANVADAARHALAAASASLLSTGTALGTKEDIARIYRELPGTIDA
>Mature_449_residues
MRGACASRAMFDTGGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASSKQQAASSKQQAASSKQ
QAASSSRPAPVLMVRRRIERKHCGACPPRSTREPRTRASRCVVFPSRIPTLKPNVTMPEIVTLTPNPAIDVATCVERVTD
TRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRAHGIDIAGETRENFSVLETSTGREF
RFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARGVRTVVDTSGPALAAALDAGVYLV
KPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVRLPGVKVAVRSAIGAGDSFVAGLVA
ALNRGANVADAARHALAAASASLLSTGTALGTKEDIARIYRELPGTIDA

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=308, Percent_Identity=38.961038961039, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788493, Length=273, Percent_Identity=32.2344322344322, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11 [C]

Molecular weight: Translated: 46298; Mature: 46298

Theoretical pI: Translated: 11.38; Mature: 11.38

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGACASRAMFDTGGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQ
CCCCHHCCHHHCCCCCCCCCCCHHHHHCCHHHHHCCHHHHHCCHHHHHCCHHHHHCCHHH
AASSKQQAASSKQQAASSKQQAASSSRPAPVLMVRRRIERKHCGACPPRSTREPRTRASR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCE
CVVFPSRIPTLKPNVTMPEIVTLTPNPAIDVATCVERVTDTRKLRCGPARRDPGGGGINV
EEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
ARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRAHGIDIAGETRENFSVLETSTGREF
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEE
RFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARGVRT
EEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEE
VVDTSGPALAAALDAGVYLVKPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALT
EECCCCCHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
LGDAGALVVSRDDAVRLPGVKVAVRSAIGAGDSFVAGLVAALNRGANVADAARHALAAAS
ECCCCEEEEECCCCEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ASLLSTGTALGTKEDIARIYRELPGTIDA
HHHHHCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRGACASRAMFDTGGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQAASGKRQ
CCCCHHCCHHHCCCCCCCCCCCHHHHHCCHHHHHCCHHHHHCCHHHHHCCHHHHHCCHHH
AASSKQQAASSKQQAASSKQQAASSSRPAPVLMVRRRIERKHCGACPPRSTREPRTRASR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCE
CVVFPSRIPTLKPNVTMPEIVTLTPNPAIDVATCVERVTDTRKLRCGPARRDPGGGGINV
EEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
ARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRAHGIDIAGETRENFSVLETSTGREF
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEE
RFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARGVRT
EEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEE
VVDTSGPALAAALDAGVYLVKPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALT
EECCCCCHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
LGDAGALVVSRDDAVRLPGVKVAVRSAIGAGDSFVAGLVAALNRGANVADAARHALAAAS
ECCCCEEEEECCCCEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ASLLSTGTALGTKEDIARIYRELPGTIDA
HHHHHCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-Fructose 6-phosphate [C]

Specific reaction: ATP + D-Fructose 6-phosphate --> ADP + D-Fructose 1,6-bisphosphate + H+ [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]