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The map label for this gene is gcvA [H]

Identifier: 126446150

GI number: 126446150

Start: 365007

End: 365930

Strand: Reverse

Name: gcvA [H]

Synonym: BMA10247_A0416

Alternate gene names: 126446150

Gene position: 365930-365007 (Counterclockwise)

Preceding gene: 126446933

Following gene: 126446541

Centisome position: 15.55

GC content: 74.89

Gene sequence:

>924_bases
ATGAACCCCTCCGATCTGCGCGACCTGCGCCTCCCGTCCCTCGACGGCTTGCTCGCCTTCGTCGCCGCCGCGCGTCACGG
CACGTTCGAGCGCGCGGCCGAGGAGCTGTGCGTGACGGGAAGCGCGGTCGCGAAGCGCGTCGCCGCGCTCGAGCAGTTGC
TCGGCGTGCAGTTGCTCGTGCGCAGCGGCAAGGCGCTCACGCTGACCGCGGCCGGCAACGAGTATCTGCACCAGGTGCAC
GCGCCGCTGCATCTGCTCGCCGCGGTGCCGCTGCATCGGCGCAACGCGGGCAAGACGGAGCGGCTGCGGATCTGCGCGCC
GCCGACGTTCGCGCGGCAGATCCTGGTTGCGCGCCTCGACGCGTTCACGCTCGCGCATCCCGGCGTGGAGCTCGAGGTCA
TGCTCGCGAGCCCCTTTCCCGACGCGCCGCCGACGGGCGCGGATCTGTGGATTCGCGGCGGCGGCCCGATCGTCTCCGGC
GCGACGGTGCTGATGGTCGATGCGCTGACGCCGCTCGCCGCGCCGTCGCTCGTCGAGCGCTTCGCGCCGTTGCGCAGGCC
CGCGGACCTCGCGGCGCTGCCGCTGTTGTGCACGCCGCTCGAGCCGTGGACGCCGTGGTTCCGCGTCGCCGGCCTCGACT
GGCCGGAGCCTGCGAACGGGCCGAAGCTGCTGGATCTCGGGCTGCTGCTCGAGGCGGCGGTCAGCGGCCAGGGCGTCGCG
CTCGGCAGCCCCGCGCTCGCGCGCCCCTGGCTCGCGGCGGGCGCGCTCGTGCCGCTGTTTCCGCGCATCGTGCGGCCGGC
GGCGCCGTATTACCTGATGCCGTACGCGCCGCGCGCCGCCGCCGGCGCGTTCGCCGCGTGGCTCGCCGACGTCTGCGAGC
GCGCGGTGCGCGACGGCGCGGAATTTCTTTCGCGCCACGGCTGA

Upstream 100 bases:

>100_bases
CGGCATCCGCTTGCATCGTGATCGCGCAATCGCGCGATCTCGCCGCTCGTCGCCGCCACGCGCCCGCCGCGGCAACAAAT
CGGTCGATTAGAATCGCCGG

Downstream 100 bases:

>100_bases
AGATATTTCCGGGCGGGCAGCCGGGCGCGGCGGTAGCATCGTCGCCACCCTCTCGGAGCCGCCCATGTCCGTCCCGATCA
CCTGCATCGAAGATCTTCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNPSDLRDLRLPSLDGLLAFVAAARHGTFERAAEELCVTGSAVAKRVAALEQLLGVQLLVRSGKALTLTAAGNEYLHQVH
APLHLLAAVPLHRRNAGKTERLRICAPPTFARQILVARLDAFTLAHPGVELEVMLASPFPDAPPTGADLWIRGGGPIVSG
ATVLMVDALTPLAAPSLVERFAPLRRPADLAALPLLCTPLEPWTPWFRVAGLDWPEPANGPKLLDLGLLLEAAVSGQGVA
LGSPALARPWLAAGALVPLFPRIVRPAAPYYLMPYAPRAAAGAFAAWLADVCERAVRDGAEFLSRHG

Sequences:

>Translated_307_residues
MNPSDLRDLRLPSLDGLLAFVAAARHGTFERAAEELCVTGSAVAKRVAALEQLLGVQLLVRSGKALTLTAAGNEYLHQVH
APLHLLAAVPLHRRNAGKTERLRICAPPTFARQILVARLDAFTLAHPGVELEVMLASPFPDAPPTGADLWIRGGGPIVSG
ATVLMVDALTPLAAPSLVERFAPLRRPADLAALPLLCTPLEPWTPWFRVAGLDWPEPANGPKLLDLGLLLEAAVSGQGVA
LGSPALARPWLAAGALVPLFPRIVRPAAPYYLMPYAPRAAAGAFAAWLADVCERAVRDGAEFLSRHG
>Mature_307_residues
MNPSDLRDLRLPSLDGLLAFVAAARHGTFERAAEELCVTGSAVAKRVAALEQLLGVQLLVRSGKALTLTAAGNEYLHQVH
APLHLLAAVPLHRRNAGKTERLRICAPPTFARQILVARLDAFTLAHPGVELEVMLASPFPDAPPTGADLWIRGGGPIVSG
ATVLMVDALTPLAAPSLVERFAPLRRPADLAALPLLCTPLEPWTPWFRVAGLDWPEPANGPKLLDLGLLLEAAVSGQGVA
LGSPALARPWLAAGALVPLFPRIVRPAAPYYLMPYAPRAAAGAFAAWLADVCERAVRDGAEFLSRHG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=32.6732673267327, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=32.6388888888889, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788706, Length=304, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081978, Length=306, Percent_Identity=28.4313725490196, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693193, Length=260, Percent_Identity=28.4615384615385, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=27.906976744186, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789639, Length=196, Percent_Identity=27.5510204081633, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=23.6933797909408, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32538; Mature: 32538

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSDLRDLRLPSLDGLLAFVAAARHGTFERAAEELCVTGSAVAKRVAALEQLLGVQLLV
CCCCCCHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
RSGKALTLTAAGNEYLHQVHAPLHLLAAVPLHRRNAGKTERLRICAPPTFARQILVARLD
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
AFTLAHPGVELEVMLASPFPDAPPTGADLWIRGGGPIVSGATVLMVDALTPLAAPSLVER
HHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHCCHHHHHH
FAPLRRPADLAALPLLCTPLEPWTPWFRVAGLDWPEPANGPKLLDLGLLLEAAVSGQGVA
HHHCCCCCHHHHHHHHCCCCCCCCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
LGSPALARPWLAAGALVPLFPRIVRPAAPYYLMPYAPRAAAGAFAAWLADVCERAVRDGA
ECCCHHHCHHHHHCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EFLSRHG
HHHHCCC
>Mature Secondary Structure
MNPSDLRDLRLPSLDGLLAFVAAARHGTFERAAEELCVTGSAVAKRVAALEQLLGVQLLV
CCCCCCHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
RSGKALTLTAAGNEYLHQVHAPLHLLAAVPLHRRNAGKTERLRICAPPTFARQILVARLD
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
AFTLAHPGVELEVMLASPFPDAPPTGADLWIRGGGPIVSGATVLMVDALTPLAAPSLVER
HHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHCCHHHHHH
FAPLRRPADLAALPLLCTPLEPWTPWFRVAGLDWPEPANGPKLLDLGLLLEAAVSGQGVA
HHHCCCCCHHHHHHHHCCCCCCCCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
LGSPALARPWLAAGALVPLFPRIVRPAAPYYLMPYAPRAAAGAFAAWLADVCERAVRDGA
ECCCHHHCHHHHHCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EFLSRHG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]