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The map label for this gene is bkdA2 [H]

Identifier: 126446124

GI number: 126446124

Start: 2230478

End: 2231521

Strand: Reverse

Name: bkdA2 [H]

Synonym: BMA10247_A2301

Alternate gene names: 126446124

Gene position: 2231521-2230478 (Counterclockwise)

Preceding gene: 126447708

Following gene: 126446955

Centisome position: 94.85

GC content: 68.2

Gene sequence:

>1044_bases
ATGACGACAGCAAGCAAAGAAGGTCCGGCGGCCTCGCCGATGACCATGATCCAGGCTTTGCGCTCGGCGATGGACGTGAT
GCTCGAGCGCGACGGCAACGTGGTGGTGTTCGGGCAGGACGTCGGCTATTTCGGCGGCGTGTTCCGCTGCACCGAAGGAC
TGCAGAACAAGTACGGCAAGTCGCGCGTGTTCGATGCGCCGATCAACGAAGGCGGCATCGTGGGCGCGGCGGTCGGGATG
GGCGCGTACGGGCTGCGCCCCGTCTGCGAGATCCAGTTCGCCGACTACTTCTATCCGGCGTCCGACCAGATCGTGTCGGA
GGCCGCGCGCCTGCGCTACCGGTCGGCGGCCGAGTTCATCGCGCCGCTGACGATCCGCATGCCGTGCGGCGGCGGCATCT
ACGGCGGCCAGACGCACAGCCAGAGCCCCGAGGCGATGTTCACGCAGGTGTGCGGGCTGCGCACGGTGATGCCGTCGAAC
CCGTACGACGCGAAGGGGCTGCTGATCTCGGCGATCGAGAACGACGATCCGGTGATCTTCCTCGAGCCGAAGCGGCTCTA
CAACGGCCCGTTCGACGGCCACCACGAACGGCCGGTGACGCCGTGGAGCAAGCATCCGGCGAGCCTCGTGCCCGACGGCT
ACTACACGGTACCGCTCGATTCGGCGGCGATCGTGCGCGCCGGCGGCGACGTGACGGTGCTCACGTACGGCACGACGGTG
CACGTATCGCTCGCGGCCGCCGAGGAAACCGGCATCGACGCCGAGGTGATCGATCTGCGCAGCCTCTGGCCGCTCGATCT
CGACACGATCGTCGAATCGGTGCGCAGGACGGGGCGCTGCGTCGTCGTGCACGAGGCGACGCGCACTTGCGGCTTCGGCG
CGGAACTCATCGCGCTCGTGCAGGAGCACTGCTTCCATTGGCTCGAGGCGCCCGTCGAGCGCGTGACCGGCTGGGACACG
CCGTATCCGCACGCGCAGGAATGGGCGTATTTCCCGGGCCCGAACCGGGTCGGCGACGCGCTGCGGCGCGCGATGGAGAA
TTGA

Upstream 100 bases:

>100_bases
ACGAGCACGTGCCGAACGTCGCGAGCATTTTCGAGGACGTCTACAAGGAGATGCCCGCGCACCTGCGCCGGCAGCGCCAA
CAGCTCGGGGTTTGACAGCG

Downstream 100 bases:

>100_bases
CATGGGCGTGCACGTTATCAAGATGCCGGACATCGGCGAAGGGATCGCGGAGGTCGAGCTCGGGCTGTGGCACGTGAAGG
TCGGCGATCGCGTGAAGGAA

Product: 2-oxoisovalerate dehydrogenase, E1 component, beta subunit

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTTASKEGPAASPMTMIQALRSAMDVMLERDGNVVVFGQDVGYFGGVFRCTEGLQNKYGKSRVFDAPINEGGIVGAAVGM
GAYGLRPVCEIQFADYFYPASDQIVSEAARLRYRSAAEFIAPLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSN
PYDAKGLLISAIENDDPVIFLEPKRLYNGPFDGHHERPVTPWSKHPASLVPDGYYTVPLDSAAIVRAGGDVTVLTYGTTV
HVSLAAAEETGIDAEVIDLRSLWPLDLDTIVESVRRTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVERVTGWDT
PYPHAQEWAYFPGPNRVGDALRRAMEN

Sequences:

>Translated_347_residues
MTTASKEGPAASPMTMIQALRSAMDVMLERDGNVVVFGQDVGYFGGVFRCTEGLQNKYGKSRVFDAPINEGGIVGAAVGM
GAYGLRPVCEIQFADYFYPASDQIVSEAARLRYRSAAEFIAPLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSN
PYDAKGLLISAIENDDPVIFLEPKRLYNGPFDGHHERPVTPWSKHPASLVPDGYYTVPLDSAAIVRAGGDVTVLTYGTTV
HVSLAAAEETGIDAEVIDLRSLWPLDLDTIVESVRRTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVERVTGWDT
PYPHAQEWAYFPGPNRVGDALRRAMEN
>Mature_346_residues
TTASKEGPAASPMTMIQALRSAMDVMLERDGNVVVFGQDVGYFGGVFRCTEGLQNKYGKSRVFDAPINEGGIVGAAVGMG
AYGLRPVCEIQFADYFYPASDQIVSEAARLRYRSAAEFIAPLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNP
YDAKGLLISAIENDDPVIFLEPKRLYNGPFDGHHERPVTPWSKHPASLVPDGYYTVPLDSAAIVRAGGDVTVLTYGTTVH
VSLAAAEETGIDAEVIDLRSLWPLDLDTIVESVRRTGRCVVVHEATRTCGFGAELIALVQEHCFHWLEAPVERVTGWDTP
YPHAQEWAYFPGPNRVGDALRRAMEN

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=338, Percent_Identity=47.0414201183432, Blast_Score=299, Evalue=2e-81,
Organism=Homo sapiens, GI34101272, Length=338, Percent_Identity=47.0414201183432, Blast_Score=299, Evalue=2e-81,
Organism=Homo sapiens, GI156564403, Length=323, Percent_Identity=36.5325077399381, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI291084858, Length=323, Percent_Identity=35.2941176470588, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17506935, Length=348, Percent_Identity=45.4022988505747, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17538422, Length=342, Percent_Identity=38.8888888888889, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=36.3914373088685, Blast_Score=212, Evalue=6e-56,
Organism=Drosophila melanogaster, GI160714832, Length=334, Percent_Identity=46.7065868263473, Blast_Score=291, Evalue=3e-79,
Organism=Drosophila melanogaster, GI160714828, Length=333, Percent_Identity=46.8468468468468, Blast_Score=291, Evalue=3e-79,
Organism=Drosophila melanogaster, GI21358145, Length=328, Percent_Identity=39.3292682926829, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24650940, Length=328, Percent_Identity=39.3292682926829, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24650943, Length=100, Percent_Identity=42, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24650945, Length=100, Percent_Identity=42, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI160714826, Length=65, Percent_Identity=47.6923076923077, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 37789; Mature: 37658

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTASKEGPAASPMTMIQALRSAMDVMLERDGNVVVFGQDVGYFGGVFRCTEGLQNKYGK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
SRVFDAPINEGGIVGAAVGMGAYGLRPVCEIQFADYFYPASDQIVSEAARLRYRSAAEFI
CCEEECCCCCCCEEEHHHCCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHH
APLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLISAIENDDPVIF
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEEECCCCCEEE
LEPKRLYNGPFDGHHERPVTPWSKHPASLVPDGYYTVPLDSAAIVRAGGDVTVLTYGTTV
ECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECCEE
HVSLAAAEETGIDAEVIDLRSLWPLDLDTIVESVRRTGRCVVVHEATRTCGFGAELIALV
EEEEEEHHCCCCCCEEEEHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHH
QEHCFHWLEAPVERVTGWDTPYPHAQEWAYFPGPNRVGDALRRAMEN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTASKEGPAASPMTMIQALRSAMDVMLERDGNVVVFGQDVGYFGGVFRCTEGLQNKYGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
SRVFDAPINEGGIVGAAVGMGAYGLRPVCEIQFADYFYPASDQIVSEAARLRYRSAAEFI
CCEEECCCCCCCEEEHHHCCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHH
APLTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLISAIENDDPVIF
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEEECCCCCEEE
LEPKRLYNGPFDGHHERPVTPWSKHPASLVPDGYYTVPLDSAAIVRAGGDVTVLTYGTTV
ECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECCEE
HVSLAAAEETGIDAEVIDLRSLWPLDLDTIVESVRRTGRCVVVHEATRTCGFGAELIALV
EEEEEEHHCCCCCCEEEEHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHH
QEHCFHWLEAPVERVTGWDTPYPHAQEWAYFPGPNRVGDALRRAMEN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3416875; 10426958 [H]