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The map label for this gene is puuR [H]

Identifier: 126446100

GI number: 126446100

Start: 339734

End: 340273

Strand: Direct

Name: puuR [H]

Synonym: BMA10247_A0384

Alternate gene names: 126446100

Gene position: 339734-340273 (Clockwise)

Preceding gene: 126446470

Following gene: 126447525

Centisome position: 14.44

GC content: 64.44

Gene sequence:

>540_bases
GTGGCGCTCGGGAGCAAGATTCGGGCGTTGCGGCAGCGGCTGAAGCGCACGCTCGACGAAACAGCGACTGCCGCGGGTAT
TTCCAAGCCGTTTTTGTCGCAGGTCGAACGCGGGCTCGCATCGCCGTCCATCACGTCGCTGGCCGGTATCGCGCACGCGC
TGGGCGTGACGGTGCAGTACTTCGTCGAGACGCCGAGCGAAGAGCGTTCGGTCTGCCGCGGCGACCAGTTGCGCTTTTTC
AGCTTCGCCGATTCGGCGAACCTGTTCGCGCGGCTGACGAACGTGCCGGAGGGGCGTCAGCTCGAGGCGATCCTCGTGCG
GATGCCGCCGGGGCAGAAGCGCTCTGAAGTGACGACGCATGCAGGCGAGGAATTCCTGTATGTGATCGAGGGTGAAGTGT
CGTTGACGCTGGAAGGCAAGTCGTTCGTGCTGCATGCCGGCGACAGCGCGCATTACCAGTCGACGGTCCCGCACAGCTGG
GTCAACACCGCGAAGATCGAGTCGGTGGTGGTCTGGGTGGGAACGCCGAGGTTGTTCTAG

Upstream 100 bases:

>100_bases
CTATCAAACTTCGTTATTTTCCATCAAACATTTTTTGACGATAAGATTTCGGCCTGCTATGGTTTCTTCCACTGAAAACA
CGCGGCTTGCCAATGCTGGC

Downstream 100 bases:

>100_bases
GGCGGGGCATTCCTTCGTGTGTTCGATGGGGTGCCTGTCGAAAGGAACGTAAGGATGACTAACATGAAATACAGGCACCA
GGGGCCCCCCGTTTCTCCGC

Product: DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQYFVETPSEERSVCRGDQLRFF
SFADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHAGDSAHYQSTVPHSW
VNTAKIESVVVWVGTPRLF

Sequences:

>Translated_179_residues
MALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQYFVETPSEERSVCRGDQLRFF
SFADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHAGDSAHYQSTVPHSW
VNTAKIESVVVWVGTPRLF
>Mature_178_residues
ALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQYFVETPSEERSVCRGDQLRFFS
FADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKSFVLHAGDSAHYQSTVPHSWV
NTAKIESVVVWVGTPRLF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=182, Percent_Identity=27.4725274725275, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 19599; Mature: 19467

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEE
FVETPSEERSVCRGDQLRFFSFADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTH
EEECCCHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHC
AGEEFLYVIEGEVSLTLEGKSFVLHAGDSAHYQSTVPHSWVNTAKIESVVVWVGTPRLF
CCCEEEEEEECEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHEEEEEEEEECCCCCC
>Mature Secondary Structure 
ALGSKIRALRQRLKRTLDETATAAGISKPFLSQVERGLASPSITSLAGIAHALGVTVQY
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEE
FVETPSEERSVCRGDQLRFFSFADSANLFARLTNVPEGRQLEAILVRMPPGQKRSEVTTH
EEECCCHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHC
AGEEFLYVIEGEVSLTLEGKSFVLHAGDSAHYQSTVPHSWVNTAKIESVVVWVGTPRLF
CCCEEEEEEECEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]