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The map label for this gene is hpaG [H]

Identifier: 126446097

GI number: 126446097

Start: 1466240

End: 1467004

Strand: Reverse

Name: hpaG [H]

Synonym: BMA10247_A1512

Alternate gene names: 126446097

Gene position: 1467004-1466240 (Counterclockwise)

Preceding gene: 126447956

Following gene: 126446976

Centisome position: 62.35

GC content: 68.24

Gene sequence:

>765_bases
ATGAGGACCGCACGCGTAGTGTTCAACGGCGCACTGCATGCGGCCGAGCCGGCGGGCGACGCGCTCGTGCGGCTCGATAC
CGGGCGCGTGCTCGCCGAGACGGAAGTCTCGTGGCTGCCGCCCGTCGTGCCGCGCACGGCGTTCGCGCTCGGCCTGAACT
ACGCCGATCACGCGAAGGAGCTCGCGTTCAGCGCGCCGGCCGAGCCGCTCATCTTCCTGAAAGGTCCGAACACGTTCATC
GGCCACCGCGCGCAAACGGTGCGACCCGCCGACGCGACGCACATGCACTATGAGTGCGAGCTCGCGGTCGTGATCGGCCG
CGAGGCGCGGCGCGTCACGCGCGCGCGGGCGCTCGACCACGTGCTCGGCTATACGATCGGCAACGATTACGCGATCCGCG
ATTATCTGGAGAATTTTTACCGACCGAACCTGCGCGTGAAGAACCGCGACACGTGCACGCCGCTCGGCCCGTGGCTCGTG
AGCCGCGACGAGGTCGGCGACGCGTCGGATCTCGCGCTGCGCACGACGGTCAACGGCGTCGAGACGCAGCGCGGCAACAC
GCGCGACATGATCTTCGACGTCGCGTCGCTGATCGAATACATCAGCGGATTCATGACGCTCTCGCCGGGCGACCTGATCC
TGACCGGCACGCCCGAGGGGCTCGCCGACACGCAGCCGGGCGACGAGGTCGTGACCGAGATCGAGGGAATCGGCCGGCTC
GTCAACACGATCGTCGGCGAAGCGGATTACTATCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
TCGGCCCGAACGCGCCGCGCGCGGCGGCGGGCAGCACGATCGAGATCGACGCGCCGGGCATCGGCACGCTGCGTCATACA
TTGGTGGCGGAGGACGCGCA

Downstream 100 bases:

>100_bases
CGCCGCCGCACAAGGAGAGACGCATGGGCATCAAGCACTGGATCGGCGGCCGCGAGGTCGACAGCCCGGAAACGTTCACG
ACGTTCAATCCGGCGACGGG

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, C-terminal subunit

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRTARVVFNGALHAAEPAGDALVRLDTGRVLAETEVSWLPPVVPRTAFALGLNYADHAKELAFSAPAEPLIFLKGPNTFI
GHRAQTVRPADATHMHYECELAVVIGREARRVTRARALDHVLGYTIGNDYAIRDYLENFYRPNLRVKNRDTCTPLGPWLV
SRDEVGDASDLALRTTVNGVETQRGNTRDMIFDVASLIEYISGFMTLSPGDLILTGTPEGLADTQPGDEVVTEIEGIGRL
VNTIVGEADYYRAG

Sequences:

>Translated_254_residues
MRTARVVFNGALHAAEPAGDALVRLDTGRVLAETEVSWLPPVVPRTAFALGLNYADHAKELAFSAPAEPLIFLKGPNTFI
GHRAQTVRPADATHMHYECELAVVIGREARRVTRARALDHVLGYTIGNDYAIRDYLENFYRPNLRVKNRDTCTPLGPWLV
SRDEVGDASDLALRTTVNGVETQRGNTRDMIFDVASLIEYISGFMTLSPGDLILTGTPEGLADTQPGDEVVTEIEGIGRL
VNTIVGEADYYRAG
>Mature_254_residues
MRTARVVFNGALHAAEPAGDALVRLDTGRVLAETEVSWLPPVVPRTAFALGLNYADHAKELAFSAPAEPLIFLKGPNTFI
GHRAQTVRPADATHMHYECELAVVIGREARRVTRARALDHVLGYTIGNDYAIRDYLENFYRPNLRVKNRDTCTPLGPWLV
SRDEVGDASDLALRTTVNGVETQRGNTRDMIFDVASLIEYISGFMTLSPGDLILTGTPEGLADTQPGDEVVTEIEGIGRL
VNTIVGEADYYRAG

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=225, Percent_Identity=39.1111111111111, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI40786394, Length=225, Percent_Identity=39.1111111111111, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI215422413, Length=199, Percent_Identity=33.1658291457286, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI66348062, Length=196, Percent_Identity=33.6734693877551, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI13654274, Length=196, Percent_Identity=33.6734693877551, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1787428, Length=191, Percent_Identity=35.0785340314136, Blast_Score=127, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17557057, Length=185, Percent_Identity=36.7567567567568, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324161, Length=225, Percent_Identity=29.3333333333333, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28572127, Length=226, Percent_Identity=40.2654867256637, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24663695, Length=221, Percent_Identity=36.1990950226244, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28571789, Length=206, Percent_Identity=33.9805825242718, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 27794; Mature: 27794

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTARVVFNGALHAAEPAGDALVRLDTGRVLAETEVSWLPPVVPRTAFALGLNYADHAKE
CCCEEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCHHHHHHHCCCHHHHHHH
LAFSAPAEPLIFLKGPNTFIGHRAQTVRPADATHMHYECELAVVIGREARRVTRARALDH
HHHCCCCCCEEEEECCCCHHCCCCCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHH
VLGYTIGNDYAIRDYLENFYRPNLRVKNRDTCTPLGPWLVSRDEVGDASDLALRTTVNGV
HHHHHCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCH
ETQRGNTRDMIFDVASLIEYISGFMTLSPGDLILTGTPEGLADTQPGDEVVTEIEGIGRL
HCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
VNTIVGEADYYRAG
HHHHHCCCCCCCCC
>Mature Secondary Structure
MRTARVVFNGALHAAEPAGDALVRLDTGRVLAETEVSWLPPVVPRTAFALGLNYADHAKE
CCCEEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCHHHHHHHCCCHHHHHHH
LAFSAPAEPLIFLKGPNTFIGHRAQTVRPADATHMHYECELAVVIGREARRVTRARALDH
HHHCCCCCCEEEEECCCCHHCCCCCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHH
VLGYTIGNDYAIRDYLENFYRPNLRVKNRDTCTPLGPWLVSRDEVGDASDLALRTTVNGV
HHHHHCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCH
ETQRGNTRDMIFDVASLIEYISGFMTLSPGDLILTGTPEGLADTQPGDEVVTEIEGIGRL
HCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
VNTIVGEADYYRAG
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA